Isolation, identification and drug sensitivity test of pathogen causing withe eye disease of Mauremys mutica

ObjectivePathogen of withe eye disease in Mauremys mutica and its drug sensitivity characteristics were confirmed in order to provide reference for prevention and control of the disease. MethodSterile anatomy, streak cul-ture, isolation and selection were applied on M. muticas with withe eye disease. Cultural characteristics and physiological and biochemical characteristics of the isolated strains were identified, 16S rDNA sequence analysis and homology compar-ison were conducted. Pathogenicity was identified through animal pathogenic experiment and drug resistance was identified through drug sensitivity test. ResultA dominant strain was isolated from liver, lung and spleen of M. mutica with withe eye disease, which was strongly pathogenic to mice and M. mutica. The isolated strain was identified to be a gram-nega-tive bacillus with facultative anaerobic, acidogenicity, aerogenesis, fermentation and no exercise ability. The 16S rDNA sequences of the isolated strain ( GenBank accession number: KU855372 ) and that of Klebsiella pneumoniae had 99.1% homology. The isolated strain was hypersensitive to enrofloxacin, cephalothin, cefotaxime and ofloxacin, but showed drug resistance to streptomycin, neomycin, penicillin, kanamycin, tetracycline, clindamycin, doxycycline, amoxicillin and compound sulfamethoxazole. ConclusionK. pneumoniae is one of the main pathogenic bacteria causing withe eye disease of M. mutica. Quinolone and cephalosporin analogues such as enrofloxacin, ofloxacin cephalothin and cefotaxime can be used to prevent and control the disease.