Simultaneous determination of eight chemical components in Oxyspora paniculata(Don) DC. using HPLC method

ObjectiveThe present study was conducted to establish high performance liquid chromatography (HPLC) method for determination of gallic acid, dihydromyricetin, protocatechuic acid, rutin, myricetrin, oleanic acid, quercetin and kaempferol in Oxyspora paniculata(Don) DC. in order to better control the quality and provide scientific basis for its medicinal development. MethodThe eight kinds of chemical components in O. paniculata from four collection sites in Du ’ an county , Guangxi ( Nongcang hamlet , Bofan hamlet , Nongwai hamlet , Baiwai hamlet ) were measured by HPLC method with ZORBAX SB-C18 column, acetonitrile-0.2% H3PO4 mobile phase, 0.6 mL/min flow rate and 28 ℃ column temperature.Result260 nm was the maximum wave lengths for determined of mixed reference substances solution and eight chemical components in O. paniculata tested solution. Contents of the eight chemical components in O. paniculata col-lected from the four collection sites were as follows: gallic acid 1 . 53-1 . 75 mg/g , protocatechuic acid 1.11-1.28 mg/g, dihydromyricetin 0.99-1.44 mg/g, rutin 0.34-0.42 mg/g, myricetrin 1.58-1.83 mg/g, oleanic acid 0.63-0.77 mg/g, quercetin 1.03-1.11 mg/g and kaempferol 0.65-0.69 mg/g. Linear relations between mass concentration and peak area and chromatographic peak separation effects of the eight chemical components were sound(r>0.9990). Sample sizes of the eight chemical components were as follows: gallic acid 7.20-43.20 μg/mL, protocatechuic acid 3.36-20.16 μg/mL, dihy-dromyricetin 5.92-35.52 μg/mL, rutin 1.60-9.60 μg/mL, myricetrin 6.40-38.40 μg/mL, oleanic acid 3.68-22.08 μg/mL, quercetin 3.20-19.20μg/mL and kaempferol 3.52-21.12μg/mL. Sample recovery rates of the eight chemical compo-nents were as follows: gallic acid 101.72%, protocatechuic acid 96.49%, dihydromyricetin 97.04%, rutin 99.79%, myricetrin 99.80%, oleanic acid 99.44%, quercetin 101.52% and kaempferol 96.63%. ConclusionThe HPLC method for O. paniculata is simple, feasible, accurate, reliable, highly sensitive and reproducible, which can be used in quality control of. O. paniculata.