Establishment of in vitro propagation technology of Alpinia zerumbet Variegata

ObjectiveThe present study was conducted to establish high-efficiency and high-frequency in vitro re-generation system of Alpinia zerumbet Variegata,in order to provide a new method for annual large-scale production of A. zerumbet seedling. MethodUsing A. zerumbet seeds as materials,the experiment on aseptic budding,callus induction, callus differentiation and rooting was carried out. ResultResults showed that, after A. zerumbet seeds were disinfected with 75%ethanol for 60 s,then 0.1%mercury chloride for 10 min,finally 5%sodium hypochlorite for 3 min,contamination rate was only 5.00%,and germination rate was 75.83%. Suitable medium for inducing callus was MS+1.50 mg/L 6-BA+0.30 mg/L 2,4-D,suitable medium for callus differentiation was MS+2.00 mg/L TDZ+0.10 mg/L 2,4-D,differentiation coef-ficient was up to 10.03. Furthermore,optimum medium for rooting was 1/2MS+1.00 mg/L ABT1 rooting powder,rooting rate was 98.00%.ConclusionIn vitro regeneration system of A. zerumbet can be established by the way of callus culture.