Genetic diversity analysis of nine chewing cane varieties(lines) and construction of their DNA fingerprints

ObjectiveIn order to provide theoretical basis for variety identification and parental selection during su-garcane breeding process,the present study was conducted to analyze genetic diversity of nine chewing cane varieties(lines) and construct their DNA fingerprints. MethodCombining twenty-one SSR molecular markers with capillary electrophore-sis technique,genetic diversities of nine chewing cane varieties(lines) from four provinces were analyzed,and genetic simi-larity coefficient between tested materials was calculated using NTSYS-pc 2.11. Then nine chewing cane varieties (lines) were clustered for analysis using UPGMA,and their DNA fingerprints were constructed. ResultResults showed that,a to-tal of 204 bands were amplified from nine chewing cane varieties(lines) using twenty-one pairs of SSR primers,with poly-morphic rate of 75.99%. So every pair of SSR primers could amplify 4-31 bands,with an average of 9.71 bands. Seventeen pairs of primer contained 44 cultivar-specific loci of nine chewing cane varieties(lines). Only five pairs of primers had the strongest ability to discriminate nine chewing cane varieties ( lines ) , and each pair of primer was able to discriminate each chewing cane varieties(lines). However,only three pairs of primers(SMC36BUQ,SMC597CS and SMC851MS),which were characterized by high polymorphism and discriminability and contained cultivar-specific loci,were chosen to con-struct DNA fingerprints of nine chewing cane varieties ( lines ) . Furethermore , genetic similarity coefficients among nine chewing cane varieties(lines) varied from 0.62 to 0.90,with an average of 0.77. UPGMA clustering result showed that,nine chewing cane materials were clustered into three groups,this result was not related to origins of materials. ConclusionThere are little differences in genetic basis,close genetic relationship and poor genetic diversity among nine chewing cane varieties(lines),so genetic background of parents should be increased in breeding process of new varieties.