Establishment of in vitro rapid propagation system of main cultivars of Shangrao early pear

ObjectiveThe present study was conducted to establish in vitro rapid propagation system of main cultivars of Shangrao early pear namely Liuyuexue and Huangpixiao, in order to provide reference for rapid propagation of test-tube plantlet of Shangrao early pear. MethodTissue culture of Liuyuexue and Huangpixiao was conducted, suitable explants, disinfection method, culture medium and transplanting matrices were selected. ResultResults showed that ideal explant of Liuyuexue and Huangpixiao was new young stems with buds of 7.0-8.0 cm in length; ideal disinfection method was 75% alcohol for 1.0 min+0.1% mercuric chloride for 10-12 min; ideal axillary bud induction medium was MS+2.0 mg/L 6-BA+0.2 mg/L IBA; ideal adventitious bud proliferation culture medium was MS+1.0 mg/L KT+0.2 mg/L NAA with coef-ficient being 3.6-3.8; ideal adventitious buds rooting culture medium was 1/2MS+0.3 mg/L NAA+0.3 mg/L PP333+0.5 g/L AC(active carbon), ideal transplanting matrix of plantlets was peat∶perlite∶vermiculite=4∶1∶1 or humus soil∶perlite=5∶1. After transplanting, watering MS culture fluid containing 0.5 mg/L PP333 could significantly improve survival rate of plantlets(P<0.05),which was up to 90.0%. ConclusionThe established in vitro rapid propagation system of Liuyuexue and Huang-pixiao can provide reference for factory production of tissue culture seedling of Shangrao early pear.