Asexually rapid propagation system established of Oriental lily Love Story

ObjectivePropagation system established with sterile leaves of Oriental lily Love Story as material to provide technical reference for industrialized production of its seed-bulbs. MethodSterile scales and leaves from flower organs culture were used as material and MS was used as basic medium, adding different concentrations of 6-BA and 2,4-D to induce adventitious buds and bulb-lets; using different concentrations of sugar to induce bulb-lets expansion and rooting; perlite, peat and vermiculite were mixed as the transplanted matrix of bulb-lets. ResultMS+1.5 mg/L 6-BA was appropriate for inducing adventitious buds with sterile scales and induction rate was 75.0% ; the suitable sterile leaves differentiation bulb -lets medium was MS +2.0 mg/L 2,4-D and induction rate was 91.7% ; the MS medium added 70.0 g/L sugar was suitable to induce bulb-lets expansion and rooting; perlite ∶peat∶vermiculite=1∶1∶1 was the best transplanted matrix of plant-lets with bulb-lets and roots, transplanting survival rate got 100.0% in this matrix.ConclusionOriental lily Love Story could be established asexually rapid propagation system by sterile leaf.