Cloning and expression analysis of tyrosylprotein sulfotransferase gene CsTPST in Cucumis sativus L.

ObjectiveThe present study was conducted to clone tyrosylprotein Sulfotransferases gene in Cucumis sativus L.(CsTPST) and analyze regulatory role of plant peptides in development of C. sativus L., in order to lay a founda-tion for analysis of function of CsTPST and interaction between peptide and ethylene in development of C. sativus L.MethodHomology alignment was conducted based on TPST protein sequence in Arabidopsis thaliana. sequence, gene structure,phylogenetic relationship,and expression pattern of CsTPST were obtained through PCR amplification and bioin-formatics analysis, and the expression pattern was studies through qRT-PCR. ResultGene cDNA in CsTPST was 789 bp in length,and encoded a protein with 262 amino acids. Phylogenetic analysis indicated that CsTPST exhibited the highest similarity with TPST from melon,with 93% of amino acid sequence similarity. qRT-PCR analysis showed that CsTPST gene had high expression level in female flowers,roots and leaves,while it had relatively low expression level in male flowers and stems. More interestingly,CsTSPT gene expression was up-related in cucumber leaves under ACC(an ethylene synthesis promoter) treatment,while down-regulated under AVG (an ethylene synthesis inhibitor) treatment. CsTPST gene pro-moter could activate expression of GFP gene. ConclusionThe results revealed that CsTSPT is an ethylene-induced gene, which can provide reference for research on biological function and molecular mechanism of sex determination.