Genetic diversity of Elaeis guineensis Jacq. based on SSR markers

ObjectiveThe present study conducted genetic structure and genetic diversity analysis on eight Elaeis guineensis Jacq. varieties using SSR molecular marker, in order to provide references for breeding through analyzing genetic structure of E. guineensis Jacq. varieties with high levels of heterozygosity. MethodUsing SSR molecular marker and PCR silver-stain technique, the researchers analyzed allele frequencies (P),observed heterozygosities (Ho) and expected het-erozygosities (He) , calculated mean number of alleles per transposons (Na) , mean number of alleles per polymorphic transposons (Na/pl) and number of effective alleles (Ne). Fixation index (Fis) and F-statistic values (Fit and Fst) were also calculated. Clustering analysis on eight E. guineensis Jacq. varieties was conducted based on genetic distance. ResultTwenty-seven pairs of polymorphic SSR primers were developed,fifteen pairs of polymorphic primers with good results were selected for testing E. guineensis Jacq. large sample. Fifty-seven alleles(Na) were detected,and Na per transposons was 3.8 on average, which showed that the genetic variation of eight E. guineensis Jacq. varieties was obvious. Average effective al-leles (Ne) was 0.6248 and expected heterozygosity (He) 0.5902. A high-level population differentiation was found, F-statistic value(Fst) ranging from 0.1029 to 0.6010 and being 0.3664 on average. Polymorphism SSR was applied to analyze genetic distance of the eight E. guineensis Jacq. varieties. The results indicated that genetic distance between Variety 1 and Variety 3 was the longest(1.674), and genetic distance between Variety 3 and Variety 5 was the shortest(0.065). Con-clusionThe eight E. guineensis Jacq. varieties enjoy abundant polymorphism, small hybridization degree between species, far species polygenetic relationship and sound genetic diversity.