Optimization for extraction process of polysaccharide from Ostrea rivularis Gould by response surface methodology

ObjectiveThe present experiment was conducted to optimize the extraction process of polysaccharide from Ostrea rivularis Gould, in order to provide a theoretical basis for comparing and analyzing content and structure of oyster polysaccharide in different sea areas. MethodThe effects of liquid-solid ratio, extraction temperature, extraction time and extraction times on extraction rate of polysaccharides from O. rivularis were investigated by single-factor test. Mean-while, based on the results of single-factor test, liquid-solid ratio, extraction time and extraction temperature was chosen as independent variables, and the extraction rate of oyster polysaccharide was selected as response value. Then the regres-sion model was established by Box-Behnken experiment design method, so as to optimize extraction process of polysaccha-ride from O. rivularis. ResultThe mathematical model for quadratic polynomial of polysaccharide extraction rate (Y) with liquid-solid ratio(A), extraction time(B), extraction temperature(C) was established by response surface method, as fol-low:Y=9.65-0.18A-0.071B+0.080C-0.27AB-0.24AC-0.52BC-2.6A2-1.34B2-1.35C2, with high fitting degree. The opti-mum conditions of extraction process were as follows:90∶1 of liquid-solid ratio, 90℃of extraction temperature, 3 h of ex-traction time, extracting 1 time. Under the above optimum conditions, the extraction rate of polysaccharide from O. rivularis reached up to 9.72%, which was close to the predicted value(9.66%), with relative deviation of 0.62%. ConclusionThe response surface methodology is a workable method to optimize extraction conditions of polysaccharide from O. rivularis, which should be applied to increase effectively extraction rate of polysaccharides in actual production.