GUO Feng, QIN De-qiang, ZENG Fu-yi, MA Yun-kun, WU Guo-xing, GAO Xi, TANG Guo-wen. 2025: Effects of three pesticides on the movement speed and protective enzyme activities of Neoseiulus californicus. Journal of Southern Agriculture, 56(7): 2176-2186. DOI: 10.3969/j.issn.2095-1191.2025.07.013
Citation: GUO Feng, QIN De-qiang, ZENG Fu-yi, MA Yun-kun, WU Guo-xing, GAO Xi, TANG Guo-wen. 2025: Effects of three pesticides on the movement speed and protective enzyme activities of Neoseiulus californicus. Journal of Southern Agriculture, 56(7): 2176-2186. DOI: 10.3969/j.issn.2095-1191.2025.07.013

Effects of three pesticides on the movement speed and protective enzyme activities of Neoseiulus californicus

  • Objective This study was to investigate the effects of three pesticides (bisultap,tetrachlorantraniliprole,and oxyfluorfen) on the movement speed and protective enzymes activity of Neoseiulus californicus,so as to provide a theoretical basis for appropriate use of pesticide,and to support the promotion and field application of Neoseiulus californicus for biological control of pest mites in citrus orchards.Method The toxicity of three pesticides (bisultap,tetrachlorantraniliprole,and oxyfluorfen) on Neoseiulus californicus was detected using medicated film method,and their safety was also evaluated. The three pesticides at sublethal concentrations (LC10,LC30 and LC50) were used to treat the feeding group and the starvation group of Neoseiulus californicus for 24 hours,with a control group treated with clear water,to measure their movement speed. The three pesticides at LC10 and LC30 were used to treat Neoseiulus californicus for 24 hours,with the control group treated with clear water,to determine enzyme activities of catalase (CAT),peroxidase (POD) and superoxide dismutase (SOD) using the spectrophotometric method.Result The LC50 values after 24-hour treatment of bisultap,tetrachlorantraniliprole and oxyfluorfen on Neoseiulus californicus were 1.626,3.818 and 0.234 g/L,respectively. The maximum safety coefficients after 24-hour treatments of bisultap,tetrachlorantraniliprole and oxyfluorfen on Neoseiulus californicus were 5.20,171.81 and 5.85,respectively,indicating that the safety order of the three pesticides for Neoseiulus californicus was tetrachlorantraniliprole>oxyfluorfen>bisultap. After the 24-hour LC50 bisultap treatment,the movement speed of Neoseiulus californicus in the feeding group was significantly higher than that of the control group (P<0.05,the same below). After the 24-hour treatment of oxyfluorfen of LC50,the movement speed of Neoseiulus californicus in the feeding group was significantly lower than that of the control group. After the 24-hour LC30 tetrachlorantraniliprole treatment,the movement speed of Neoseiulus californicus in the feeding group was significantly lower than that in the control group and the LC50 treatment group. After the 24-hour LC10 tetrachlorantraniliprole treatment,the movement speed of Neoseiulus californicus in starvation group was significantly lower than that in the control group. No significant difference in total protein content was found in Neoseiulus californicus after the 24-hour treatments of LC10 and LC30 of the three pesticides (P>0.05,the same below). No significant difference in CAT activity of Neoseiulus californicus was found between the control group and the 24-hour LC10 and LC30 bisultap treatment groups. Under the 24-hour LC10 tetrachlorantraniliprole treatment,the CAT activity of of Neoseiulus californicus was significantly lower than that in the control group and the 24-hour LC30 tetrachlorantraniliprole treatment group. Under the 24-hour LC10 and LC30 treatments of bisultap and tetrachlorantraniliprole,the POD activity of Neoseiulus californicus was significantly lower than that in the control group. Under the 24-hour LC30 treatments of bisultap and tetrachlorantraniliprole,the SOD activity of Neoseiulus californicus was significantly higher than that of the control group. Under the 24-hour LC10 and LC30 oxyfluorfen treatments,the activities of three protective enzymes of Neoseiulus californicus was significantly lower than that of the control group.Conclusion The three pesticides (bisultap,tetrachlorantraniliprole,and oxyfluorfen) influence the athletic ability of Neoseiulus californicus. Tetrachlorantraniliprole inhibits the CAT of Neoseiulus californicus; bisultap and tetrachlorantraniliprole inhibit POD but activate SOD of Neoseiulus californicus. Oxyfluorfen significantly inhibits activities of the three protective enzymes of Neoseiulus californicus. Therefore,tetrachlorantraniliprole,which is rather safe for Neoseiulus californicus,is recommended to be applied for integrated management of cirtus orchards,so as to achieve the synergistic effect of biological control and chemical control.
  • loading

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return