Effects of methyl jasmonate treatment on biosynthesis of ferulic acid and expression of JAZ gene family in Angelica sinensis （Oliv.）Diels

【Objective】To eplore the effects of methyl jasmonate（MeJA）treatment on the biosynthesis of ferulic acid and the expression of jasmonate ZIM-domain（JAZ）gene family in Angelica sinensis（Oliv.）Diels，which could provide theoretical basis for further research on the biosynthesis regulation mechanism of ferulic acid in Angelica sinensis（Oliv.） Diels.【Method】Angelica sinensis（Oliv.）Diels seedlings were materials，and the Angelica sinensis（Oliv.）Diels seedlings were treated with 100 μmol/L MeJA，and samples were taken at time gradients（0，1.5，3.0，9.0 and 12.0 h）. High performance liquid chromatography（HPLC）was used to detect the content of ferulic acid in the roots，stems，and leaves of A.sinensis at different treatment times. JAZ gene family members were screened from the genome of Angelica sinensis （Oliv.）Diels and bioinformatics analysis on the encoded protein AsJAZs physicochemical properties，conserved domains， subcellular localization prediction were conducted. The relative expression levels of the AsJAZs gene after MeJA treatment were detected by real-time fluorescence quantitative PCR.【Result】The ferulic acid content in the roots and stems of Angelica sinensis（Oliv.）Diels showed an overall trend of first increasing and then decreasing under MeJA treatment， while the ferulic acid content in the leaves showed an increasing trend. A total of 11 JAZ gene family members（AsJAZ1-AsJAZ11）were screened from the genome of Angelica sinensis（Oliv.）Diels，encoding 136-586 amino acids，and most of which were alkaline proteins. Subcellular localization predicted that AsJAZ3 proteins were located on chloroplasts，while the rest proteins were located in nucleus. The 11 AsJAZs were divided into 4 subfamilies，containing a total of 5 conserved domains，including the typical tify domain and jas_motif domain，and the domain composition of AsJAZs protein in the same subfamily was highly similar. After treatment with exogenous MeJA，the relative expression levels of 11 AsJAZs in the roots，stems，and leaves of Angelica sinensis（Oliv.）Diels showed a trend of first increasing and then decreasing. Among them，the relative expression level of AsJAZ8 gene was positively correlated with ferulic acid content.【Conclusion】When exogenous MeJA is used to treat seedlings of Angelica sinensis（Oliv.）Diels，it can affect the biosynthesis of ferulic acid in the roots，stems，and leaves of Angelica sinensis（Oliv.）Diels for a certain period of time，and the AsJAZs genes mainly responds to MeJA in the stems and leaves. Among them，the AsJAZ8 may be a key transcriptional regulation gene that responds to MeJA signals and participates in regulating the biosynthesis of ferulic acid.