Volume 56 Issue 2
Feb.  2025
Turn off MathJax
Article Contents
Abstract
References
Related Articles
CHEN Yu-jin, YU Han, ZHU Hua-li, JIANG Tao, LIU Bo, LI Wei, ZHAO Yong-jun, LU Yi-zeng, JIE Xiao-man. 2025: Identification of TCP gene family in Xanthoceras sorbifolium and expression analysis under salt stress. Journal of Southern Agriculture, 56(2): 441-451. DOI: 10.3969/j.issn.2095-1191.2025.02.009
Citation: CHEN Yu-jin, YU Han, ZHU Hua-li, JIANG Tao, LIU Bo, LI Wei, ZHAO Yong-jun, LU Yi-zeng, JIE Xiao-man. 2025: Identification of TCP gene family in Xanthoceras sorbifolium and expression analysis under salt stress. Journal of Southern Agriculture, 56(2): 441-451. DOI: 10.3969/j.issn.2095-1191.2025.02.009
shu

Identification of TCP gene family in Xanthoceras sorbifolium and expression analysis under salt stress

  • 1 College of Landscape and Forestry, Qingdao Agricultural University/Shandong Key Laboratory for Germplasm Innovation of Saline-alkaline Tolerant Grasses and Trees, Qingdao, Shandong 266109, China;

  • 2 Jinan Lily Landscape Group Co., Ltd., Jinan, Shandong 250014, China;

  • 3 Key Laboratory of State Forestry and Grassland Administration on Conservation and Utilization of Warm Temperate Zone Forest and Grass Germplasm Resources/Shandong Center of Forest and Grass Germplasm Resources, Jinan, Shandong 250102, China

Funds: 

Shandong Key Research and Development Plan(Agricultural Elite Variety Project) Project(2020LZGC0904)

Innovation Team of Rare Tree Species Conservation and Utilization Project of Shandong Natural Resources Department(LZRZZ[2023]98)

More Information
  • Received Date: September 18, 2024
    Graphical Abstract
    • Abstract
  • 【Objective】 The TCP gene family of Xanthoceras sorbifolium was identified and its expression under salt stress was analyzed,its response mechanism to salt stress was studied,which provided theoretical basis for breeding of salt-tolerant germplasm of X.sorbifolium.【Method】The TCP gene family of X.sorbifolium was identified by bioinformatics method,and the protein physicochemical properties,phylogenetic evolution,conserved motifs,gene structure,chromo‐some localization and promoter cis-acting elements were analyzed.Real-time fluorescence quantitative PCR was used to detect the expression of TCP genes of X.sorbifolium（XsTCPs） in leaf and root under salt stress. 【Result】 A total of 24members of the TCP gene family（XsTCP1-XsTCP24） of X.sorbifolium were identified,encoding 185-576 amino acids residues,protein molecular weight being 20576.21-63366.92 kD,theoretical isoelectric point being 4.62-10.25,all of which were hydrophilic proteins and located in the nucleus.Based on the phylogenetic analysis results,the 24 XsTCPs proteins could be divided into 3 subfamilies,among which the PCF subfamily contained 14 members,the CIN subfamily contained 6 members,and the CYC/TB1 subfamily contained 4 members.Except that XsTCP23 and XsTCP24 genes were unable to locate on the chromosome,the remaining TCP gene family members were unevenly distributed on 11 chromo‐somes in the form of single genes or gene clusters.A total of 10 conserved motifs were found in 24 XsTCPs proteins,among which Motif 1 was common to all proteins.The TCP gene family members of X.sorbifolium contained light response elements,hormone response elements,stress response elements and plant growth and development elements.XsTCP4,XsTCP6,XsTCP7,XsTCP10,XsTCP12,XsTCP13,XsTCP21 and XsTCP22 genes contained drought-induced MYB binding elements.The results of real-time fluorescence quantitative PCR showed that the expression of the above genes changed greatly in the leaves and roots of X.sorbifolium under salt stress,suggesting that the above 8 XsTCPs genes were involved in the process of salt stress response. 【Conclusion】 XsTCPs proteins may be involved in biological pro‐cesses by regulating various target genes involved in growth,development,metabolism and stress response.It is specu‐lated that 8 XsTCPs genes containing drought-induced MYB binding elements may help improve the resistance to salt stress.
    • FullText(HTML)
    • References (38)
  • 樊兴路.2015.文冠果无性系早期选择研究及幼苗对盐碱胁迫的响应[D].太谷:山西农业大学.[Fan X L.2015.The clone early selection of Xanthoceras sorbifolia Bunge and the response of seedlings to salinity-alkalinity stress[D].Taigu:Shanxi Agricultural University.]
    关紫微,曹希雅,张先文,周小云.2022.水稻TCP家族的全基因组鉴定及表达分析[J].分子植物育种,20(10):3145-3156.[Guan Z W,Cao X Y,Zhang X W,Zhou X Y.2022.Genome-wide identification and expression analysis of TCP family in rice(Oryza sativa L.)[J]Molecular Plant Breeding,20(10):3145-3156.] doi: 10.13271/j.mpb.020.003145.
    侯元凯,刘松杨,黄琳,周海江.2009.我国生物柴油树种选择与评价[J].林业科学研究,22(1):7-13.Hou Y K,Liu S Y,Huang L,Zhou H J.2009.Selection and evaluation of bio-diesel tree species in China[J].Forest Research,22(1):7-13.] doi: 10.3321/j.issn:1001-1498.2009.01.002.
    雷静,班秀文,刘荣,王健,陈红燕,龙佩霖,杨成龙.2023.薏苡(Coix Lacryma-jobiL.)TCP家族全基因组鉴定及响应非生物胁迫的表达规律[J/OL].分子植物育种. https://kns.cnki.net/kcms/detail/46.1068.S.20230510.1438.004.html" target="_blank"> https://kns.cnki.net/kcms/detail/46.1068.S.20230510.1438.004.html.[Lei J,Ban X W,Liu R,Wang J,Chen H Y,Long P L,Yang C L.2023.Genome-wide identification and expression analysis of TCP gene family under abiotic stress in Coix Lacryma-jobi L.[J/OL].Molecular Plant Breeding. https://kns.cnki.net/kcms/detail/46.1068.S.20230510.143 8.004.html.]
    雷其冬.2021.拟南芥miR319-TCP4调控植物应答干旱胁迫的分子机制研究[D].昆明:昆明理工大学.[Lei Q D.2021.The molecular mechanism of miR319-TCP4 regulating plant drought response in Arabidopsis thaliana[D].Kunming:Kunming University of Scienceand Technology.]doi: 10.27200/d.cnki.gkmlu.2021.001625.
    李佳皓,谢敏秋,万传银,左瑞洁,鲁黎明,李立芹.2021.马铃薯转录因子StTCP13基因的原核表达及盐胁迫功能研究[J].华北农学报,36(2):33-39.[Li J H,Xie M Q,Wan C Y,Zuo R J,Lu L M,Li L Q.2021.Prokaryotic expression of transcription factor StTCP13 in potato and its function of salt stress[J].Acta Agriculturae Boreali-Sinica,36(2):33-39.] doi:10.7668/hbnxb.201915 07.
    李茂.2014.山西北中部文冠果生物质能源林建设规划研究[D].太谷:山西农业大学.[Li M.2014.Research on the construction plan of Xanthoceras sorbifolium biomass energy forest in north central Shanxi Province[D].Taigu:Shanxi Agricultural University.]
    刘欢龙.2020.毛竹TCP转录因子的鉴定及PeTCP10基因的功能分析[D].合肥:安徽农业大学.Liu H L.2020.Identification of TCP transcription factors in bamboo and functional analysis of PeTCP10 gene[D].Hefei:Anhui Agricultural University] doi: 10.26919/d.cnki.gannu.2020.000873.
    米跃骐,田彦挺,杨浩,邓永平,孙宇涵,李云.2023.刺槐TCP基因家族鉴定及表达分析[J/OL].分子植物育种. https://kns.cnki.net/kcms/detail/46.1068.S.20230505.123 8.008.html.[Mi Y Q,Tian Y T,Yang H,Deng Y P,Sun Y H,Li Y. 2023.Identification and expression analysis of TCP gene family in Robinia pseudoacacia[J/OL].Molecular Plant Breeding. https://kns.cnki.net/kcms/detail/46.1068.S.20230505.1238.008.html.]
    宋宇英,江敏,王世杰,张卫强,刘洪山,任亚超,张军.2023.蒙古栎TCP基因家族全基因组鉴定与分析[J/OL].分子植物育种. https://link.cnki.net/urlid/46.1068.s.20230814.1022.002" target="_blank"> https://link.cnki.net/urlid/46.1068.s.20230814.1022.002.[Song Y Y,Jiang M,Wang S J,Zhang W Q,Liu H S,Ren Y C,Zhang J.2023.Genome-wide identification and analysis of TCP gene family in Quercus mongolica[J/OL].Molecular Plant Breeding. https://link.cnki.net/urlid/46.1068.s.2023 0814.1022.002.]
    唐羽翔,高旭,崔亚宁,许会敏,于静娟.2022.植物TCP转录因子研究进展[J].科学通报,67(33):3964-3975.[Tang Y X,Gao X,Cui Y N,Xu H M,Yu J J.2022.Research advances in the plant TCP transcription factors[J].Chinese Science Bulletin,67(33):3964-3975.] doi: 10.1360/TB-2022-0480.
    王峰.2011.生物质能源林的首选树种--文冠果[J].甘肃林业,(2):37-38.[Wang F.2011.The preferred tree species for biomass energy forests-Xanthoceras sorbifolium[J].Forestry of Gansu,(2):37-38.]
    王景超,张君,齐云,于晓菲,程彬,于军,张娟.2022.玉米TCP家族基因的表达分析[J].玉米科学,30(1):63-68.[Wang J C,Zhang J,Qi Y,Yu X F,Cheng B,Yu J,Zhang J.2022.Expression analysis on TCP family genes of maize[J].Journal of Maize Sciences,30(1):63-68.] doi: 10.13597/j.cnki.maize.science.20220109.
    王世泽,李云,韩玉翠,余世洲,王爽,刘勇,林小虎.2024.烟草TCP家族成员鉴定及表达分析[J].生物工程学报,40(1):226-238.[Wang S Z,Li Y,Han Y C,Yu S Z,Wang S,Liu Y,Lin X H.2024.Identification and expression analysis of TCP family members in tobacco(Nicotiana tabacum L.)[J].Chinese Journal of Biotechnology,40(1):226-238.] doi: 10.13345/j.cjb.230345.
    张龙,徐世强,李静宇,胡学博,顾艳,王继华.2024.穿心莲TCP基因家族全基因组鉴定及非生物胁迫下的表达分析[J].中国中药杂志,49(2):379-388.[ZhangL,Xu S Q,Li J Y,Hu X B,Gu Y,Wang J H.2024.Genome-wide identification and expression analysis of TCP gene family of Andrographis paniculata under abiotic stress[J].China Journal of Chinese Materia Medica,49(2):379-388.] doi: 10.19540/j.cnki.cjcmm.20230906.102.
    曾家晶,赵怡娇,曾东琳,唐为玲,李尊文,Wang-Pruski Gefu,赖钟雄,郭容芳.2022.芥蓝TCP家族全基因组鉴定及表达分析[J].中国农业大学学报,27(8):76-92.ZengJJ,Zhao Y J,Zeng D L,Tang W L,Li Z W,Wang-Pruski G F,Lai Z X,Guo R F.2022.Genome identification and expression analysis of TCP family in Chinese kale[J].Journal of China Agricultural University,27(8):76-92.] doi: 10.11841/j.issn.1007-4333.2022.08.08.
    周瑾,李洁,李晓燕,肖丹怡,李梦瑶.2022.芹菜TCP基因家族全基因组鉴定、进化分析和表达研究[J].四川农业大学学报,40(2):145-155.[Zhou J,Li J,Li X Y,Xiao D Y,Li M Y.2022.Genome-wide identification,evolution andexpression analysis of TCP gene family in celery(Apium graveolens L.)[J].Journal of Sichuan Agricultural University,40(2):145-155.] doi: 10.16036/j.issn.1000-2650.202110039.
    Artimo P,Jonnalagedda M,Arnold K,Baratin D,Csardi G,de Castro E,Duvaud S,Flegel V,Fortier A,Gasteiger E,Grosdidier A,Hernandez C,Ioannidis V,Kuznetsov D,Liechti R,Moretti S,Mostaguir K,Redaschi N,Rossier G,Xenarios I,Stockinger H.2012.ExPASy:SIB bioinformatics resource portal[J].Nucleic Acids Research,40(W1):W597-W603.doi: 10.1093/nar/gks400.
    Chen C J,Chen H,Zhang Y,Thomas H R,Frank M H,He Y H,Xia R.2020.TBtools:An integrative toolkit developed for interactive analyses of big biological data[J].Molecular Plant,13(8):1194-1202.doi: 10.1016/j.molp.2020.06.009.
    Cheng Z H,Lei N,Li S X,Liao W B,Shen J,Peng M.2019.The regulatory effects of MeTCP4 on cold stress tolerance in Arabidopsis thaliana:A transcriptome analysis[J].Plant Physiology and Biochemistry,138:9-16.doi: 10.1016/j.plaphy.2019.02.015.
    Cubas P,Lauter N,Doebley J,Coen E.1999.The TCP domain:A motif found in proteins regulating plant growth and development[J].The Plant Journal,18(2):215-222.doi: 10.1046/j.1365-313X.1999.00444.x.
    Doebley J,Stec A,Gustus C.1995.Teosinte branchedl and the origin of maize:Evidence for epistasis and the evolution of dominance[J].Genetics,141(1):333-346.doi:10.1093/genetics/141.1.3 3 3.
    El-Gebali S,Mistry J,Bateman A,Eddy S R,Luciani A,Potter S C,Qureshi M,Richardson L J,Salazar G A,Smart A,Sonnhammer E L L,Hirsh L,Paladin L,Piovesan D,Tosatto S C E,Finn R D.2019.The Pfam protein families database in 2019[J].Nucleic Acids Research,47(D1):D427-D432.doi:10.109 3/nar/gky995.
    Francis A,Dhaka N,Bakshi M,Jung K H,Sharma M K,Sharma R.2016.Comparative phylogenomic analysis provides insights into TCP gene functions in Sorghum[J].Scientific Reports,6(1):38488.doi:10.103 8/srep3 8488.
    Huang X Y,Liu G,Zhang W W.2018.Genome-wide analysis of LBD(LATERAL ORGAN BOUNDARIES Domain)gene family in Brassica rapa[J].Brazilian Archives of Biology and Technology,61:e18180049.doi: 10.1590/1678-4324-2018180049.
    Li S T,Zachgo S.2013.TCP3 interacts with R2R3-MYB proteins,promotes flavonoid biosynthesis and negatively regulates the auxin response in Arabidopsis thaliana[J].The Plant Journal,76(6):901-913.doi: 10.1111/tpj.12348.
    Li S T.2015.The Arabidopsis thaliana TCP transcription factors:A broadening horizon beyond development[J].Plant Signaling&Behavior,10(7):e1044192.doi: 10.1080/15592324.2015.1044192.
    Lin Y F,Chen Y Y,Hsiao Y Y,Shen C Y,Hsu J L,Yeh C M,Mitsuda N,Ohme-Takagi M,Liu Z J,Tsai W H.2016.Genome-wide identification and characterization of TCP genes involved in ovule development of Phalaenopsis equestris[J].Journal of Experimental Botany,67(17):5051-5066.doi: 10.1093/jxb/erw273.
    Ling L,Zhang W R,An Y M,Du B H,Wang D,Guo C H.2020.Genome-wide analysis of the TCP transcription factor genes in five legume genomes and their response to salt and drought stresses[J].Functional&Integrative Genomics,20(4):537-550.doi: 10.1007/s10142-020-00733-0.
    Liu Y J,An J P,Gao N,Wang X,Chen X X,Wang X F,Zhang S,You C X.2022.MdTCP46 interacts with MdABI5 to negatively regulate ABA signalling and drought response in apple[J].Plant,Cell&Environment,45(11):3233-3248.doi: 10.1111/pce.14429.
    Luo D,Carpenter R,Vincent C,Copsey L,Coen E.1996.Origin of floral asymmetry in Antirrhinum[J].Nature,383(6603):794-799.doi: 10.1038/383794a0.
    Martín-Trillo M,Cubas P.2010.TCP genes:A family snapshot ten years later[J].Trends in Plant Science,15(1):31-39.doi: 10.1016/j.tplants.2009.11.003.
    Meng J X,Yin J,Wang H,Li H H.2022.A TCP transcription factor in Malus halliana,MhTCP4,positively regulates anthocyanins biosynthesis[J].International Journal of Molecular Sciences,23(16):9051.doi:10.3390/ij ms23169051.
    Mishra S,Sahu G,Shaw B P.2022.Insight into the cellular andphysiological regulatory modulations of Class-I TCP9 to enhance drought and salinity stress tolerance in cowpea[J].Physiologia Plantarum,174(1):e13542.doi: 10.1111/ppl.13542.
    Rueda-Romero P,Barrero-Sicilia C,Gómez-Cadenas A,Carbonero P,Oñate-Sánchez L.2012.Arabidopsis thaliana DOF6 negatively affects germination in non-after-ripened seeds and interacts with TCP14[J].Journal of Experimental Botany,63(5):1937-1949.doi: 10.1093/jxb/err388.
    Shen J J,Zhang Y Q,Ge D F,Wang Z Y,Song W Y,Gu R,Che G,Cheng Z H,Liu R Y,Zhang X L.2019.CsBRC1inhibits axillary bud outgrowth by directly repressing the auxin efflux carrier CsPIN3 in cucumber[J].Proceedings of the National Academy of Sciences of the United States of America,116(34):17105-17114.doi: 10.1073/pnas.1907968116.
    Wang K T,Zhang N,Fu X,Zhang H H,Liu S Y,Pu X,Wang X,Si H J.2022.StTCP15 regulates potato tuber sprouting by modulating the dynamic balance between abscisic acid and gibberellic acid[J].Frontiers in Plant Science,13:1009552.doi:10.3 389/fpls.2022.1009552.
    Zhou M,Li D Y,Li Z G,Hu Q,Yang C H,Zhu L H,Luo H.2013.Constitutive expression of a miR319 gene alters plant development and enhances salt and drought tolerance in transgenic creeping bentgrass[J].Plant Physiology,161(3):1375-13 91.doi: 10.1104/pp.112.208702.
    • Related Articles

  • Related Articles

    YANG Fan, ZOU Chang-li, ZHANG Li-chao, DU Qing-jie, LI Juanqi, WANG Hu, WANG Ji-qing, XIAO Huai-juan, LI Meng. 2025: Identification of CmLEA gene family and mining of abiotic stress resistant genes in melon. Journal of Southern Agriculture, 56(2): 563-572. DOI: 10.3969/j.issn.2095-1191.2025.02.020
    ZHU Hua-li, CHEN Yu-xia, QIAN Cheng, CHEN Yu-jin, YU Han, JIANG Tao, LI Lulu, LU Yi-zeng. 2025: Identification of HD-Zip gene family members in Xanthoceras sorbifolium Bunge and their response to salt stress. Journal of Southern Agriculture, 56(2): 378-391. DOI: 10.3969/j.issn.2095-1191.2025.02.004
    YU Han, YANG Lei, JIANG Tao, LIU Bo, QIAN Cheng, CHEN Yu-jin, ZHU Hua-li, LI Wei, WANG Jing-cai. 2024: Identification of LBD gene family in Xanthoceras sorbifolium and its response to salt stress. Journal of Southern Agriculture, 55(11): 3333-3345. DOI: 10.3969/j.issn.2095-1191.2024.11.013
    QIAN Chao-nan, XU Sun-ran, LI Meng-ru, DENG Ming-chao, SUN Shuo, LIU Guo-yuan, LIAN Bo-lin, ZHANG Jian, CHEN Yan-hong. 2024: Whole genomic identification of NAC gene family and expression analysis under flooding stress and salt stress in Salix matsudana. Journal of Southern Agriculture, 55(10): 3056-3070. DOI: 10.3969/j.issn.2095-1191.2024.10.018
    SHI Lu-xi, TIAN Huai-zhi, XIONG Xing-wei, MU Yuan-hang, ZHANG Ju, HE Fang, ZHANG Qing-qin, ZHANG Su-qin. 2023: Cloning and expression characteristics of ethylene receptor gene TtETR1 in response to salt stress in Tritipyrum. Journal of Southern Agriculture, 54(1): 13-21. DOI: 10.3969/j.issn.2095-1191.2023.01.002
    WANG Qi-zhang, ZHANG Xiang-lin, HAN Rui, TIAN Jie. 2022: Screening of garlic reference genes and analysis of AsACO gene response to salt stress and plant growth-promoting rizhobacteria. Journal of Southern Agriculture, 53(12): 3297-3306. DOI: 10.3969/j.issn.2095-1191.2022.12.001
    LI Ya-ya, SU Huan-huan, MA Dong-mei, FAN Jia-jia, ZHU Hua-ping. 2022: Effects of salt-alkali stress on TauT2 gene expression in Oreochromis mossambicus. Journal of Southern Agriculture, 53(10): 2787-2796. DOI: 10.3969/j.issn.2095-1191.2022.10.009
    ZHANG Qi, ZHANG Ai-xia, JIA Jun-ting, ZHAO Hua, LIU Jun, CHEN Bing-xian. 2022: Rice WOX family gene identification and expression analysis of seeds germinated under stress. Journal of Southern Agriculture, 53(7): 1809-1820. DOI: 10.3969/j.issn.2095-1191.2022.07.003
    LI Wei-jin, ZHONG Cai-rong, ZHANG Ying, YUAN Chang-chun, LI Ren-mao. 2022: Analysis of the root transcriptomes in Sesuvium portulacastrum respond to salt stress. Journal of Southern Agriculture, 53(3): 693-703. DOI: 10.3969/j.issn.2095-1191.2022.03.011
    PANG Dan-dan, LIU Yu-fei, TIAN Yi-ping, SUN Yun-nan, CHEN Lin-bo. 2021: Identification and expression analysis of ZF-HD transcription factor gene family in Camellia sinensis. Journal of Southern Agriculture, 52(3): 632-640. DOI: 10.3969/j.issn.2095-1191.2021.03.011

Catalog

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (1) PDF downloads (0) Cited by()
    Related

    Copyright ©Editorial Department of Southern Journal of Agriculture

    Address：174 Daxue Dong Lu, Nanning City, Guangxi Province, ChinaChina Pos：530007

    Tel：（+86）-771-3243905;（+86）-771-3244920Email：nfnyxb@163.com

    Supported by: Beijing Renhe Information Technology Co., Ltd. 

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return