Volume 55 Issue 10
Oct.  2024
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TAN Yong-yan, ZENG Shuai-bo, LU An-bin, TANG Hua-jiang, YANG Hao, PENG Li-sha, SHEN Si, LIU Ren-xiang. 2024: Mechanisms of overdominance gene in the formation of biomass heterosis in tobacco leaves based on transcriptomics analysis. Journal of Southern Agriculture, 55(10): 2966-2979. DOI: 10.3969/j.issn.2095-1191.2024.10.010
Citation: TAN Yong-yan, ZENG Shuai-bo, LU An-bin, TANG Hua-jiang, YANG Hao, PENG Li-sha, SHEN Si, LIU Ren-xiang. 2024: Mechanisms of overdominance gene in the formation of biomass heterosis in tobacco leaves based on transcriptomics analysis. Journal of Southern Agriculture, 55(10): 2966-2979. DOI: 10.3969/j.issn.2095-1191.2024.10.010
shu

Mechanisms of overdominance gene in the formation of biomass heterosis in tobacco leaves based on transcriptomics analysis

  • 1 Tobacco College, Guizhou University/Key Laboratory of Tobacco quality Research of Guizhou, Guiyang, Guizhou 550025, China;

  • 2 Tongren Company, Guizhou Tobacco Company, Tongren, Guizhou 554300, China

Funds: 

National Natural Science Foundation of China（32060510）；Guizhou Tobacco Company Project （2022XM02）；Bijie Tobacco Company Project （2024520500240064）

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  • Received Date: June 03, 2024
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    • Abstract
  • 【Objective】 This study aimed to elucidate the mechanisms by which overdominance gene contributed to biomass heterosis in tobacco leaves through transcriptomic analysis，thereby providing theoretical reference for the breeding of high-yield hybrids. 【Method】 The study utilized a hybrid（K326×GDH88）exhibiting biomass heterosis and its parental lines as experimental materials. By measuring the biomass of the tobacco parents and the hybrid，the critical period for heterosis formation was identified. Transcriptome sequencing technology was employed to analyze differentially expressed genes（DEGs）associated with biomass heterosis in tobacco leaves and their expression patterns. 【Result】 Positive heterosis in biomass was observed in the hybrid between 45 and 52 d after transplantation. The mid-parent advantage was the highest between 45 and 52 d post-transplantation. During this period，the gene expression model of the hybrid shifted from an additive mid-parent model to a positive dominance model，indicating that 45 d post-transplantation was the critical period for analyzing biomass formation in hybrid tobacco leaves. Transcriptome sequencing of samples collected 45 d after transplantation revealed 2660 DEGs between the hybrid and its parents. Of these，1683 DEGs（63.27%） exhibited overdominant expression，which was likely the primary cause of biomass heterosis in tobacco leaves. Among the overdominantly expressed DEGs，28 were up-regulated and enriched in photosynthesis-related pathways，while 10 were down-regulated and enriched in sucrose and starch metabolism pathway related to respiration. These DEGs with overdominant expression in photosynthesis and respiration were potential key candidate genes regulating biomass heterosis in hybrid. 【Conclusion】 The critical period for the formation of biomass heterosis in tobacco hybrid leaves is 45 d posttransplantation. The formation of biomass heterosis is primarily influenced by the overdominance effect of DEGs. The overdominant up-regulation and down-regulation of related DEGs in photosynthesis and respiration may be the underlying reasons for biomass heterosis in tobacco leaves.
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  • 曹立勇,占小登,庄杰云,郑康乐,程式华. 2003. 水稻产量性状的QTL定位与上位性分析[J]. 中国农业科学,36(11): 1241-1247.[Cao L Y,Zhan X D,Zhuang J Y,Zheng K L, Cheng S H. 2003. QTL mapping and epistasis analysis for yield components in a RIL population of rice (Oryza sativa L. subsp. Indica) [J]. Scientia Agricultura Sinica,36(11): 1241-1247.] doi: 10.3321/j.issn:0578-1752.2003.11.002.
    曾帅波. 2023. 杂交种烟叶生物量优势形成的生理及分子基础研究[D]. 贵阳:贵州大学.[Zeng S B. 2023. Physiological and molecular basis research on the formation of biomass dominance in hybrid tobacco leaves[D]. Guiyang:Guizhou University.]
    陈泽辉. 2009. 群体与数量遗传学[M]. 贵阳:贵州科技出版社.[Chen Z H. 2009. Population and quantitative genetics[M]. Guiyang:Guizhou Science and Technology Press.]
    董文科,马祥,张玉娟,郭睿,张兆恒,张岩,马晖玲. 2019. 低温胁迫对不同早熟禾品种糖酵解代谢及其相关基因表达的影响[J]. 草地学报,27(6): 1503-1510.[Dong W K, Ma X,Zhang Y J,Guo R,Zhang Z H,Zhang Y,Ma H L. 2019. Effects of low temperature stress on glycolysis metabolism and related gene expression of different Poa pratensis varieties[J]. Acta Agrestia Sinica,27(6): 1503-1510.] doi: 10.11733/j.ISSN.1007-0435.2019.06.006.
    梁婷,阙远慧,姜超英,贾傛吏,段丽丽,黄莺,刘洋,刘仁祥. 2023. 烤烟品种韭菜坪2 号叶片数的遗传分析[J]. 山地农业生物学报,42(1): 70-74.[Liang T,Que Y H,Jiang C Y,Jia R L,Duan L L,Huang Y,Liu Y,Liu R X. 2023.Genetic analysis of leaf number of flue-cured tobacco variety Jiucaiping No. 2[J]. Journal of Mountain Agriculture and Biology,42(1): 70-74.] doi:10.15958/j.cnki.sdnyswxb. 2023.01.010.
    刘国侠,冉法芬,李云,薛琳,李世金,朱启法,季学军,刘旦. 2018. 皖南地区不同烤烟种质产量产值与农艺性状的相关分析[J]. 科技通报,34(2): 46-50.[Liu G X,Ran F F, Li Y,Xue L,Li S J,Zhu Q F,Ji X J,Liu D. 2018. Analysis of correlation between yield and production value and agronomic traits in different varieties of flue-cured tobacco[J]. Bulletin of Science and Technology,34(2): 46-50.]doi:10.13774/j.cnki.kjtb.2018. 02. 011.
    刘仁祥,夏志林,崔庆伟,喻奇伟,张庆明,聂琼,徐秀红,刘洋. 2016. 贵州烤烟育种工作进展与对策探讨[J]. 山地农业生物学报,35(4): 1-6.[Liu R X,Xia Z L,Cui Q W, Yu Q W,Zhang Q M,Nie Q,Xu X H,Liu Y. 2016. Progress in tobacco breeding in Guizhou Province and discussion for solutions[J]. Journal of Mountain Agricultural and Biology,35(4): 1-6.] doi:10.15958/j.cnki.sdnyswxb.2016. 04.001.
    陆星星,龚兰芳,李明福,董军忠. 2012. 不同栽培条件与烤烟产质量关系分析[J]. 山地农业生物学报,31(3): 231-234.[Lu X X,Gong L F,Li M F,Dong J Z. 2012. Analysis of relationship between different cultivation conditions and yield and quality of flue-cured tobacco[J]. Journal of Moun-tain Agricultural and Biology,31(3): 231-234.] doi:10. 15958/j.cnki.sdnyswxb.2012.03.017.
    罗雯. 2022. 基于转录组学的烟叶钾含量杂种优势形成机理研究[D]. 贵阳:贵州大学.[Luo W. 2022. Study on the formation mechanism of heterosis of potassium content in tobacco leaves based on transcriptome[D]. Guiyang:Guizhou University.]
    努尔凯麦尔·木拉提,杨亚杰,帕尔哈提·阿布都克日木,玛依努尔·吾斯曼. 2021. 小麦叶绿素含量测定方法比较[J]. 江苏农业科学,49(9): 156-159.[Murat N,Yang Y J, Abudukerimu P,Osman M. 2021. Comparative study on determination methods of chlorophyll content in wheat[J]. Jiangsu Agricultural Sciences,49(9): 156-159.] doi:10. 15889/j.issn.1002-1302.2021.09. 028.
    皮凯. 2023. 烟草杂交种根系数量优势相关基因分析[D]. 贵阳:贵州大学.[Pi K. 2023. Analysis of genes related to heterosis of tobacco root number[D]. Guiyang:Guizhou University.]
    孙其信,倪中福,陈希勇,刘志勇,黄铁城.1997. 冬小麦部分基因杂合性与杂种优势表达[J]. 中国农业大学学报, 2(1): 64.[Sun Q X,Ni Z F,Chen X Y,Liu Z Y,Huang T C. 1997. Heterozygosity and heterosis expression of some genes in winter wheat[J]. Journal of China Agricultural University,2(1): 64.]
    谭彪,任慕瑶,杨正鹏,徐佳依,郑华斌,唐启源,王慰亲. 2024. GA3和KNO2引发处理对水稻芽期生长、淀粉及呼吸代谢的影响[J]. 中国稻米, 30(3): 40-47.[Tan B,Ren M Y,Yang Z P,Xu J Y,Zheng H B,Tang Q Y,Wang W Q. 2024. Effects of GA 3 priming and KNO2 priming on the seedling establishment,starch metabolism and respiratory metabolism of rice[J]. China Rice,30(3): 40-47.] doi: 10.3969/j.issn.1006-8082.2024.03.006.
    唐志明,马荣荣,王晓燕,陆永法,周华成,蔡克峰. 2021. 甬优系列籼粳杂交组合产量优势与亲本籼粳成分及农艺性状间的关系研究[J]. 杂交水稻,36(5): 65-72.[Tang Z M,Ma R R,Wang X Y,Lu Y F,Zhou H C,Cai K F. 2021.
    Relationship of yield heterosis of Yongyou series of indicajaponica hybrid combinations to the genetic distance and agronomical traits of parents[J]. Hybrid Rice,36(5): 65-72.] doi: 10.16267/j.cnki.1005-3956.20200612.199.
    王国琴. 2015. 烟草烟碱杂种优势表现及其基因差异表达分析[D]. 贵阳:贵州大学.[Wang G Q. 2015. Studies on tobacco nicotine trait of heterosis and related gene differential expression analysis[D]. Guiyang:Guizhou University.]
    魏锋,史大坤,卫晓轶,马毅,洪德峰,马俊峰,王稼苜,郑秋道. 2022. 不同玉米杂交种产量和品质性状的杂种优势分析[J]. 山东农业科学, 54(12): 25-30.[Wei F,Shi D K,Wei X Y,Ma Y,Hong D F,Ma J F,Wang J M,Zheng Q D. 2022. Heterosis analysis of yield and quality characters of different maize hybrids[J]. Shandong Agricultural Science,54(12): 25-30.] doi:10.14083/j.issn.1001-4942. 2022.12.004.
    魏明月,云菲,刘国顺,宋亮. 2017. 不同光环境下烟草光合特性及同化产物的积累与分配机制[J]. 应用生态学报,28(1): 159-168.[Wei M Y,Yun F,Liu G S,Song L. 2017.Response of photosynthetic characteristics and accumulation and distribution of assimilation products in tobacco to different light environments[J]. Chinese Journal of Applied Ecology,28(1): 159-168.] doi:10.13287/j.1001-9332. 201701.010.
    尹剑藤,李佛琳,杨焕文,段树苍,陈茂建,李成杰,蔡寒玉. 2011. 10 个烤烟新品种田间性状及叶片组织结构的比较[J]. 中国农学通报,27(10): 92-97.[Yin J T,Li F L, Yang H W,Duan S C,Chen M J,Li C J,Cai H Y. 2011.Field property and leaf dissecting structure comparison in ten new flue-cured tobacco cultivars[J]. Chinese Agricultural Science Bulletin,27(10): 92-97.]
    悦飞雪,李继伟,王艳芳,刘杉,王小东,陈明灿,刘领. 2019.不同基因型烤烟对镉、铅富集特征评价[J]. 江苏农业科学, 47(8): 105-111.[Yue F X,Li J W,Wang Y F,Liu S, Wang X D,Chen M C,Liu L. 2019. Evaluation of accumulation characteristics of cadmium and lead in tobacco with different genotypes[J]. Jiangsu Agricultural Science,47(8): 105-111.] doi: 10.15889/j.issn.1002-1302.2019.08.024.
    张爱民,刘冬成,聂秀玲,郭小丽,黄铁城. 2002. 杂种小麦育种的战略[J]. 中国农业科技导报,(5): 42-48.[Zhang A M,Liu D C,Nie X L,Guo X L,Huang T C. Strategy of hybrid wheat breeding[J]. Journal of Agricultural Science and Technology,(5): 42-48.] doi:10.3969/j.issn.1008-0864. 2002.05.011.
    Cannell M G R,Thornley J H M. 2000. Modelling the components of plant respiration:Some guiding principles[J]. Annals of Botany,85(1): 45-54.
    Ernst J,Bar-Joseph Z. 2006. STEM:A tool for the analysis of short time series gene expression data[J]. BMC Bioinformatics, 7:191. doi: 10.1186/1471-2105-7-191.
    Fugate K K,Eide J D,Lafta A M,Tehseen M M,Chu C G, Khan M F R,Finger F L. 2024. Transcriptomic and metabolomic changes in postharvest sugarbeet roots reveal widespread metabolic changes in storage and identify genes potentially responsible for respiratory sucrose loss[J]. Frontiers in Plant Science,15:1320705. doi: 10.3389/fpls.2024.1320705.
    Hasegawa K,Yukawa Y,Sugita M,Sugiura M. 2002. Organization and transcription of the gene family encoding chlorophyll a/b-binding proteins in Nicotiana sylvestris[J]. Gene,289(1-2): 161-168. doi:10.1016/S0378-1119(02) 00539-5.
    Hauben M,Haesendonckx B,Standaert E,Van Der Kelen K, Azmi A,Akpo H,Van Breusegem F,Guisez Y,Bots M, Lambert B,Laga B,De Block M. 2009. Energy use efficiency is characterized by an epigenetic component that can be directed through artificial selection to increase yield[J]. Proceedings of the National Academy of Sciences of the United States of America,106(47): 20109-20114. doi: 10.1073/pnas.0908755106.
    Huang X H,Zhao Y,Wei X H,Li C Y,Wang A H,Zhao Q,Li W J,Guo Y L,Deng L W,Zhu C R,Fan D L,Lu Y Q, Weng Q J,Liu K Y,Zhou T Y,Jing Y F,Si L Z,Dong G J, Huang T,Lu T T,Feng Q,Qian Q,Li J Y,Han B. 2011.
    Genome-wide association study of flowering time and grain yield traits in a worldwide collection of rice germplasm[J]. Nature Genetics,44(1): 32-39. doi:10.1038/ng. 1018.
    Huang Z B,Ye J,Zhai R R,Wu M M,Yu F M,Zhu G F,Wang Z F,Zhang X M,Ye S H. 2023. Comparative transcriptome analysis of the heterosis of salt tolerance in intersubspecific hybrid rice[J]. International Journal of Molecular Sciences,24(3): 2212. doi: 10.3390/IJMS24032212.
    Jones D F. 1917. Dominance of linked factors as a means of accounting for heterosis[J]. Genetics,2(5): 466-479. doi: 10.1093/genetics/2.5.466.
    Joshi J,Amthor J S,McCarty D R,Messina C D,Wilson M A, Millar A H,Hanson A D. 2023. Why cutting respiratory CO2 loss from crops is possible,practicable,and prudential[J]. Modern Agriculture,1(1): 16-26. doi: 10.1002/moda.1.
    Li G Y,Chen T T,Feng B H,Peng S B,Tao L X,Fu G F. 2021.Respiration,rather than photosynthesis,determines rice yield loss under moderate high-temperature conditions[J]. Frontiers Plant Science,12:678653. doi:10.3389/fpls.2021. 678653.
    Li L Z,Lu K Y,Chen Z M,Mu T M,Hu Z L,Li X Q. 2008.Dominance,overdominance and epistasis condition the heterosis in two heterotic rice hybrids[J]. Genetics,180(3): 1725-1742. doi:10.1534/geneti cs.108.091942.
    Liu Y M,Qu J T,Zhang L,Xu X Y,Wei G,Zhao Z F,Ren M Z,Cao M J. 2019. Identification and characterization of the TCA cycle genes in maize[J]. BMC Plant Biology,19(1): 592. doi: 10.1186/s12870-019-2213-0.
    Livak K J,Schmittgen T D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T) ) [J]. Methods,25(4): 402-408. doi: 10.1006/meth.2001.1262.
    Mo Z J,Luo W,Pi K,Duan L L,Wang P S,Ke Y Z,Zeng S B, Jia R L,Liang T,Huang Y,Liu R X. 2022. Comparative transcriptome analysis between inbred lines and hybrids provides molecular insights into K+ content heterosis of tobacco (Nicotiana tabacum L.) [J]. Frontiers in Plant Science, 13:940787. doi: 10.3389/fpls.2022.940787.
    Nejat N,Ramalingam A,Mantri N. 2018. Advances in transcriptomics of plants[J]. Adv BiochemEng Biotechnol, 164:161-185. doi: 10.1007/10_2017_52.
    Pâques E L,García-Casas M D C,Charpentier J P. 2013. Distribution of heartwood extractives in hybrid larches and in their related European and Japanese larch parents:Relationship with wood colour parameters[J]. European Journal of Forest Research,132(1): 61-69. doi: 10.1007/s10342-012-0654-1.
    Rapp R A,Udall J A,Wendel J F. 2009. Genomic expression dominance in allopolyploids[J]. BMC Biology,7(1): 18.doi: 10.1186/1741-7007-7-18.
    Shang L G,Liang Q Z,Wang Y M,Zhao Y P,Wang K B,Hua J P. 2016. Epistasis together with partial dominance,overdominance and QTL by environment interactions contribute to yield heterosis in upland cotton[J]. Theoretical and Applied Genetics,129(7): 1429-1446. doi: 10.1007/s00122-016-2714-2.
    Sitaramam V,Bhate R,Kamalraj P,Pachapurkar S. 2008. Respiration hastens maturation and lowers yield in rice[J]. Physiology and Molecular Biology of Plants,14(3): 253-271. doi:10.1007/s1229 8-008-0024-0.
    Stuber C W,Lincoln S E,Wolff D W,Helentjaris T,Lander E S. 1992. Identification of genetic factors contributing to heterosis in a hybrid from two elite maize inbred lines using molecular markers[J]. Genetics,132(3): 823-839.doi: 10.1093/genetics/132.3.823.
    Stupar R M,Gardiner J M,Oldre A G,Haun W J,Chandler V L,Springer N M. 2008. Gene expression analyses in maize inbreds and hybrids with varying levels of heterosis[J]. BMC Plant Biology,8(1): 33. doi: 10.1186/1471-2229-8-33.
    Wang L F,Li J,Lin Y,Dang K T,Wan J,Meng S J,Qiu X Q, Wang Q Y,Mu L Q,Ding D,Luo H B,Tang J H. 2022.Comparative transcriptomics analysis at the key stage of maize ear development dissect heterosis[J]. The Plant Genome, 16(1): e20293. doi: 10.1002/tpg2.20293.
    Xiong J,Hu K N,Shalby N,Zhuo C J,Wen J,Yi B,Shen J X, Ma C Z,Fu T D,Tu J X. 2022. Comparative transcriptomic analysis reveals the molecular mechanism underlying seedling biomass heterosis in Brassica napus[J]. BMC Plant Biology,22(1): 283. doi: 10.1186/S12870-022-03671-0.
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