Volume 55 Issue 10
Oct.  2024
Turn off MathJax
Article Contents
Abstract
References
LI Meng, XIE Yun-xiao, WANG Kai, HAN Jing-mei, HE Xiao-jian, XIAO Dong, MA Lin, RAO Zhi. 2024: Microbial community structure and function of Yunnan cigar tobacco leaves based on high-throughput sequencing. Journal of Southern Agriculture, 55(10): 2957-2965. DOI: 10.3969/j.issn.2095-1191.2024.10.009
Citation: LI Meng, XIE Yun-xiao, WANG Kai, HAN Jing-mei, HE Xiao-jian, XIAO Dong, MA Lin, RAO Zhi. 2024: Microbial community structure and function of Yunnan cigar tobacco leaves based on high-throughput sequencing. Journal of Southern Agriculture, 55(10): 2957-2965. DOI: 10.3969/j.issn.2095-1191.2024.10.009
shu

Microbial community structure and function of Yunnan cigar tobacco leaves based on high-throughput sequencing

  • 1 College of Tobacco Science and Engineering, Zhengzhou University of Light Industry, Zhengzhou, Henan 450001, China;

  • 2 Technology Center of China Tobacco Yunnan Industrial Co., Ltd., Kunming, Yunnan 650231, China;

  • 3 Hongyun Honghe Tobacco(Group)Co., Ltd., Kunming, Yunnan 650231, China

Funds: 

National Natural Science Foundation of China（21706244）；Key Laboratory Project of China National Tobacco Corporation（110201503003）；Key Project of China Tobacco Yunnan Industrial Co.，Ltd（. JB2022YL02）

More Information
  • Received Date: June 06, 2024
    Graphical Abstract
    • Abstract
  • 【Objective】 To explore the microbial community structure and diversity of cigar tobacco leaves after agricultural fermentation in different producing areas and uses in Yunnan，which could provide reference for screening functional microorganisms of cigar tobacco leaves and improving the fermentation quality of cigar tobacco leaves. 【Method】 The microbial community structure was analyzed based on high-throughput sequencing technology，and the metabolic functions of the microbial community were analyzed and predicted using cigar tobacco leaves with different uses（cigar wrapper，filler and binder leaves）from 3 origins，namely Dehong，Lincang and Pu’er in Yunnan，as the test materials. 【Result】 Operation taxonomic unit（OTU）cluster analysis was performed according to 97% similarity，and a total of 2393 OTUs were obtained. The number of OTUs in each sample ranged from 125 to 442，and there was a large difference between samples. The Chao1 index，ACE index and Shannon index of tobacco leaf samples of different uses from the same origin were different，all showed binder > wrapper and filler leaves. Comparing tobacco leaf samples for the same use but from different origins，the 3 indexes of tobacco leaf samples from Dehong and Pu’er were higher than those of Lincang tobacco samples，with more abundant microbial species. The microorganisms of cigar tobacco leaves from different producing areas were mainly bacteria，and the dominant phylum were Proteobacteria and Firmicutes. There were great differences in the dominant genera of tobacco leaves with different uses，with Klebsiella occupying a larger proportion of the cigar wrapper leaves from 3 origins，Pantoea dominated in the filler leaves from Lincang and Pu’er；and the dominant genera of the binder leaves from 3 origins were more diverse and abundant. The results of KEEG and COG function prediction analysis showed that the microbial functional metabolic pathways and functional types of 9 tobacco samples were basically the same，but the relative abundance was greatly different. KEEG function prediction metabolic pathways were mainly global and overview maps，carbohydrate metabolism，amino acid metabolism，and the COG function type prediction was mainly distributed in the unknown function，amino acid transport and metabolism，transcription， inorganic ion transport and metabolism，carbohydrate transport and metabolism. 【Conclusion】 The use of tobacco leaves is an important factor affecting the microbial diversity of cigar tobacco leaves in Yunnan，while the influence of producing area is relatively small，and the microbial richness of binder leaves is higher than that of cigar wrapper and filler leaves.
    • FullText(HTML)
    • References (36)
  • 陈栋,李猛,王荣浩,李林林,李秀妮,时向东. 2019. 国产雪茄茄芯烟叶研究进展[J]. 扬州大学学报(农业与生命科学版), 40(1): 83-90.[Chen D,Li M,Wang R H,Li L L,Li X N,Shi X D. 2019. Progress of the domestic cigar filler tobacco[J]. Journal of Yangzhou University(Agricultural and Life Science Edition), 40(1): 83-90.] doi: 10.16872/j.cnki.1671-4652.2019.01.015.
    陈音,孙贤,郑召君,蔡文,薛芳,刘元法,李东亮,刘路路. 2023. 加料发酵对茄芯烟叶化学成分及表面细菌多样性的影响[J]. 中国烟草学报,29(1): 106-115.[Chen Y, Sun X,Zheng Z J,Cai W,Xue F,Liu Y F,Li D L,Liu L L. 2023. Influences of feed fermentation on chemical compositions and bacterial diversity of cigar filler tobacco[J]. Acta Tabacaria Sinica,29(1): 106-115.] doi: 10.16472/j.chinatobacco.2021.064.
    韩凤,林茂祥,章文伟,李巧玲,肖忠,谭秋生,杨毅,李品明. 2021. 多花黄精根腐病对根际土壤酶活性及真菌群落变化的影响[J]. 西南大学学报(自然科学版), 43(4): 53-61.[Han F,Lin M X,Zhang W W,Li Q L,Xiao Z,Tan Q S,Yang Y,Li P M. 2021. Effects of Polygonatum cyrtonema root rot on rhizosphere soil enzyme activity and fungal community[J]. Journal of Southwest University (Natural Science), 43(4): 53-61.] doi:10.13718/j.cnki.xdzk.2021. 04.007.
    黄阔,李栋,王彬,刘克建,陈宸,米强,王庚斗,马铭赛,史占东,孟庆华,范黎,叶长文. 2023a. 手工雪茄加工过程中细菌群落多样性与游离氨基酸含量的相关性分析[J]. 烟草科技,56(7): 8-16.[Huang K,Li D,Wang B,Liu K J, Chen C,Mi Q,Wang G D,Ma M S,Shi Z D,Meng Q H, Fan L,Ye C W. 2023a. Correlation between bacterial community diversity and free amino acid content during handrolled cigar processing[J]. Tobacco Science & Technology, 56(7): 8-16.] doi:10.16135/j.issn1002-0861.2022. 0818.
    黄阔,李栋,王彬,余君,刘克建,李青常,米强,孟庆华,范黎, 叶长文. 2023b. 雪茄烟叶工业发酵过程中细菌群落多样性和结构变化[J]. 烟草科技,56(5): 17-24.[Huang K, Li D,Wang B,Yu J,Liu K J,Li Q C,Mi Q,Meng Q H, Fan L,Ye C W. 2023b. Variations of bacterial community diversity and structure of cigar tobacco during industrial fermentation[J]. Tobacco Science & Technology,56(5): 17-24.] doi: 10.16135/j.issn1002-0861.2022.0207.
    雷亚芳. 2021. 不同酶制剂与香料处理对烟叶品质及微生物多样性的影响[D]. 杨凌:西北农林科技大学.[Lei Y F. 2021. Effects of different enzyme preparations and spice treatments on the quality and microbial diversity of tobacco leaves[D]. Yangling:Northwest A&F University.] doi:10. 27409/d.cnki.gxbnu.2021.000988.
    黎旺姐,李勇,崔明昆,张刚,龚明. 2016. 烟草氨基酸代谢及其调控机制[J]. 安徽农业科学,44(21): 15-19.[Li W J, Li Y,Cui M K,Zhang G,Gong M. 2016. Amino acid metabolism and its regulation mechanism in tobacco plants[J]. Journal of Anhui Agricultural Sciences,44(21): 15-19.] doi: 10.13989/j.cnki.0517-6611.2016.21.005.
    李石头,潘凡达,黄晓玉,闫茗熠,田雨农,廖付,王辉,吴继忠. 2023. 基于宏基因组学的不同陈化时间烟草源功能微生物筛选[J]. 轻工学报,38(1): 101-109.[Li S T,Pan F D,Huang X Y,Yan M Y,Tian Y N,Liao F,Wang H,Wu J Z. 2023. Screening of tobacco-derived functional microorganisms with different aging time based on metagenomics[J]. Journal of Light Industry,38(1): 101-109.]doi: 10.12187/2023.01.012.
    刘超,李奇,宋子洋,胡鹏,敬双怡,李卫平. 2023. 低温下磁性载体 MBBR系统微生物群落特征和功能预测分析[J]. 环境科学,44(2): 889-899.[Liu C,Li Q,Song Z Y,Hu P, Jing S Y,Li W P. 2023. Analysis of microbial community characteristics and function prediction of MBBR with magnetic biocarriers at low temperature[J]. Environmental Science,44(2): 889-899.] doi: 10.13227/j.hjkx.202203078.
    刘福童,李茂森,陈敏,张赛,张福建,王生才,赵培炎,宋安东,方明,任天宝,刘国顺. 2022. 高碳基土壤修复肥对烤烟根际土壤细菌群落结构及烟叶产值的影响[J]. 河南农业大学学报,56(5): 724-731.[Liu F T,Li M S,Chen M, Zhang S,Zhang F J,Wang S C,Zhao P Y,Song A D,Fang M,Ren T B,Liu G S. 2022. Effects of high carbon-based soil remediation fertilizer on bacterial community structure and output value of tobacco leaves in flue-cured tobacco rhizosphere soil[J]. Journal of Henan Agricultural University, 56(5): 724-731.] doi:10.16445/j.cnki.1000-2340.2022 0825.001.
    刘慧,赵松超,武志勇,赵铭钦. 2021. 不同采收方式对雪茄烟上部叶晾制效果的影响[J]. 中国烟草学报,27(3): 65-72.[Liu H,Zhao S C,Wu Z Y,Zhao M Q. 2021. Effects of different harvesting methods on upper leaves air-curing of cigar[J]. Acta Tabacaria Sinica,27(3): 65-72.] doi: 10.16472/j.chinatobacco.2020.t0060.
    刘京伟,李香真,姚敏杰. 2021. 植物根际微生物群落构建的研究进展[J]. 微生物学报,61(2): 231-248.[Liu J W,Li X Z,Yao M J. 2021. Research progress on assembly of plant rhizosphere microbial community[J]. Acta Microbiologica Sinica,61(2): 231-248.] doi:10.13343/j.cnki.wsxb. 20200154.
    刘天波,赵誉强,滕凯,周向平,肖艳松,蔡海林,肖志鹏,陈武,唐前君. 2022. 烟草疫霉拮抗细菌B44R-1 的筛选及其生防效果[J]. 中国生物防治学报,38(6): 1545-1552.[Liu T B,Zhao Y Q,Teng K,Zhou X P,Xiao Y S,Cai H L,Xiao Z P,Chen W,Tang Q J. 2022. Screening and biocontrol effect of antagonistic bacterium B44R-1 against Phytophthora parasitica[J]. Chinese Journal of Biological Con-trol,38(6): 1545-1552.] doi:10.16409/j.cnki.2095-039x. 2022.06.012.
    米其利,李雪梅,管莹,高茜,桂永发,朱洲海,夭建华. 2016.高通量测序在食品微生物生态学研究中的应用[J]. 食品科学,37(23): 302-308.[Mi Q L,Li X M,Guan Y,Gao Q, Gui Y F,Zhu Z H,Yao J H. 2016. Application of highthroughput sequencing in food microbial ecology:A review[J]. Food Science,37(23): 302-308.] doi:10.7506/spkx 1002-6630-201623049.
    宋学茹,张立猛,卜令铎,黄智华,瞿兴,陈文华,张继来,苏友波. 2022. 云南不同产地醇化雪茄烟叶微生物群落差异研究[J]. 中国烟草科学,43(5): 87-93.[Song X R,Zhang L M,Bu L D,Huang Z H,Qu X,Chen W H,Zhang J L,Su Y B. 2022. Study on microbial community of alcoholized cigar leaves from different producing areas of Yunnan Province[J]. Chinese Tobacco Science,43(5): 87-93.] doi: 10.13496/j.issn.1007-5119.2022.05.014.
    孙明辉,孙会忠,李广良,朱金峰,陈冲. 2015. 1 株兼有烟碱降解活性解磷菌的分离鉴定[J]. 基因组学与应用生物学, 34(10): 2176-2180.[Song M H,Sun H Z,Li G L,Zhu J F,Chen C. 2015. Isolation and identification of a phosphorus solubilizing strain with nicotine degradation activity[J]. Genomics and Applied Biology,34(10): 2176-2180.]doi: 10.13417/j.gab.034.002176.
    唐杰,陈知青,郭安南,裘琼芬. 2021. 不同作物根际土壤微生物的群落结构特征分析[J]. 核农学报,35(12): 2830-2840.[Tang J,Chen Z Q,Guo A N,Qiu Q F. 2021. Characteristics of microbial community structure in the rhizosphere soil of different crops[J]. Journal of Nuclear Agricultural Sciences,35(12): 2830-2840.] doi:10.11869/j.issn. 100-8551.2021.12.2830.
    田丽君,荊永锋,郑卜凡,彭新辉,龚嘉蕾,吴涛,李正风,李振杰,田祥珅,周启运,龚嘉,黎娟. 2022. 浏阳洞库陈化烟叶微生物多样性分析[J]. 中国烟草学报,28(1): 98-107.[Tian L J,Jing Y F,Zheng B F,Peng X H,Gong J L,Wu T,Li Z F,Li Z J,Tian X S,Zhou Q Y,Gong J,Li J. 2022.Analysis of microbial diversity of aged tobacco leaves in Liuyang Cave[J]. Acta Tabacaria Sinica,28(1): 98-107.]doi: 10.16472/j.chinatobacco.2020.T0088.
    王琰琰,刘国祥,向小华,王俊,张瑞娜,佟英,李媛,戴培刚, 张兴伟,吕洪坤. 2020. 国内外雪茄烟主产区及品种资源概况[J]. 中国烟草科学,41(3): 93-98.[Wang Y Y,Liu G X,Xiang X H,Wang J,Zhang R N,Tong Y,Li Y,Dai P G,Zhang X W,Lü H K. 2020. Overview of main cigar production areas and variety resources at domestic and overseas[J]. Chinese Tobacco Science,41(3): 93-98.] doi:10. 13496/j.issn.1007-5119.2020.03.016.
    吴丽君,张鸽,陈晓娜,张瑶,金永明,段如敏,邓帅军,白晓莉,王帆,张海波. 2023. 云南雪茄烟叶人工发酵过程中化学成分与细菌群落变化及其相关性分析[J]. 中国烟草学报,29(4): 124-134.[Wu L J,Zhang G,Chen X N, Zhang Y,Jin Y M,Duan R M,Dang S J,Bai X L,Wang F, Zhang H B. 2023. Changes in chemical composition and bacterial communities during fermentation of Yunnan cigar tobacco leaves and their correlation[J]. Acta Tabacaria Sinica, 29(4): 124-134.] doi: 10.16472/j.chinatobacco.2022.T0220.
    徐慧,党立志,杨根华,王明锋,夏建军,李青青,段焰青. 2013. 烟碱降解菌的筛选、鉴定及其降解性能的初步研究[J]. 云南大学学报(自然科学版), 35(4): 580-586.[Xu H,Dang L Z,Yang G H,Wang M F,Xia J J,Li Q Q, Duan Y Q. 2013. A preliminary study on the screening, identification and degradation characteristics of nicotine degradation bacterium strain[J]. Journal of Yunnan University(Natural Sciences Edition), 35(4): 580-586.] doi: 10.7540/j.ynu.20120414.
    徐伟,葛阳阳,陈翠婷,马婷婷. 2018. 基于宏基因组技术分析传统红茶菌中菌群组成及其主要代谢通路[J]. 食品工业科技,39(5): 119-123.[Xu W,Ge Y Y,Chen C T,Ma T T. 2018. Microoraanism composition and main metabolic pathways analysis of traditional Kombucha by metagenomic technology[J]. Science and Technology of Food Industry,39(5): 119-123.] doi:10.13386/j.issn1002-0306. 2018.05.023.
    叶科媛,刘路路,卢瑞琳,向欢,刘仡,胡希,曾祥华,王俊. 2022. 不同成熟度和晾制方式对雪茄烟叶品质的影响[J]. 浙江农业科学,63(7): 1584-1587.[Ye K Y,Liu L L, Lu R L,Xiang H,Liu Y,Hu X,Zeng X H,Wang J. 2022.Effect of maturity and air-curing methods on the quality of cigar tobacco leaves[J]. Journal of Zhejiang Agricultural Sciences,63(7): 1584-1587.] doi:10.16178/j. issn. 0528-9017.20212990.
    张倩颖,罗诚,李东亮,蔡文. 2020. 雪茄烟叶调制及发酵技术研究进展[J]. 中国烟草学报,26(4): 1-6.[Zhang Q Y, Luo C,Li D L,Cai W. 2020. Research progress in curing and fermentation technology for cigar tobacco leaf production[J]. Acta Tabacaria Sinica,26(4): 1-6.] doi: 10.16472/j.chinatobacco.2019.339.
    郑天飞,张倩颖,李东亮,张娟,堵国成. 2022. 不同产地雪茄烟叶的风味特征和微生物群落分析[J]. 河南农业科学, 51(8): 45-54.[Zheng T F,Zhang Q Y,Li D L,Zhang J, Du G C. 2022. Analysis of flavor characteristics and microbial community of cigar tobacco leaves from different regions[J]. Journal of Henan Agricultural Sciences,51(8): 45-54.] doi: 10.15933/j.cnki.1004-3268.2022.08.006.
    郑天飞. 2022. 雪茄烟叶发酵过程中微生物群落结构和功能分析[D]. 无锡:江南大学.[Zheng T F. 2022. Analysis of microbial community structure and function during fermentation of cigar tobacco leaves[D]. Wuxi:Jiangnan University.] doi: 10.27169/d.cnki.gwqgu.2022.002338.
    Ansari W A,Kumar M,Krishna R,Singh A,Zeyad M T,Tiwari P,Kumar S C,Chakdar H,Srivastava A K. 2023. Influence of rice-wheat and sugarcane-wheat rotations on microbial diversity and plant growth promoting bacteria:Insights from high-throughput sequencing and soil analysis[J]. Microbiological Research,278:127533. doi:10.1016/j.micres. 2023.127533.
    Blake B,Brady L I,Rouse N A,Nagy P,Tarnopolsky M A. 2023. The efficacy of whole genome sequencing and RNASeq in the diagnosis of whole exome sequencing negative patients with complex neurological phenotypes[J]. Journal of Pediatric Ggenetics,12(3): 206-212. doi: 10.1055/S-0041-1736610.
    Chattopadhyay S,Smyth E M,Kulkarni P,Babik K R,Reid M, Hittle L E,Clark P I,Mongodin E F,Sapkota A R. 2019.Little cigars and cigarillos harbor diverse bacterial communities that differ between the tobacco and the wrapper[J]. PLoS One,14(2): e0211705. doi:10.1371/journal.pone.02 11705.
    Hu W R,Cai W,Zheng Z J,Liu Y F,Luo C,Xue F,Li D L. 2022. Study on the chemical compositions and microbial communities of cigar tobacco leaves fermented with exogenous additive[J]. Scientific Reports,12(1): 19182. doi: 10.1038/S41598-022-23419-Y.
    Li J J,Zhao Y Y,Qin Y Q,Shi H Z. 2020. Influence of microbiota and metabolites on the quality of tobacco during fermentation[J]. BMC Microbiology,20(1): 356. doi:10. 1186/s12866-020-02035-8.
    Liu F,Wu Z Y,Zhang X P,Xi G L,Zhao Z,Lai M,Zhao M Q. 2021. Microbial community and metabolic function analysis of cigar tobacco leaves during fermentation[J]. Microbiology Open,10(2): e1171. doi: 10.1002/MBO3.1171.
    Smyth E M,Chattopadhyay S,Babik K,Reid M,Chopyk J, Malayil L,Kulkarni P,Hittle L E,Clark P I,Sapkota A R, Mongodin E F. 2019. The bacterial communities of little cigars and cigarillos are dynamic over time and varying storage conditions[J]. Frontiers in Microbiology,10:2371.doi: 10.3389/fmicb.2019.02371.
    Wu Q Y,Peng Z,Pan Y,Liu L P,Li L L,Zhang J,Wang J. 2023. Interaction analysis of tobacco leaf microbial community structure and volatiles flavor compounds during cigar stacking fermentation[J]. Frontiers in Microbiology, 14:1168122. doi: 10.3389/FMICB.2023.1168122.
    Zhang Q,Kong G H,Zhao G K,Liu J,Jin H G,Li Z H,Zhang G H,Liu T. 2023. Microbial and enzymatic changes in cigar tobacco leaves during air-curing and fermentation[J]. Applied Microbiology and Biotechnology,107(18): 5789-5801. doi: 10.1007/S00253-023-12663-5.
    Zhao Y,Zhong G F,Yang X P,Hu X M,Mao D B,Ma Y P. 2015. Bioconversion of lutein to form aroma compounds by Pantoea dispersa[J]. Biotechnology Letters,37(8): 1687-1692. doi: 10.1007/s10529-015-1844-y.
    • Related Articles

Catalog

    Get Citation
    PDF
    XML

    Article Metrics

    Article has an altmetric score of 2
    Article has an altmetric score of 2
    Article views (163) PDF downloads (15) Cited by()
    Related

    Copyright ©Editorial Department of Southern Journal of Agriculture

    Address：174 Daxue Dong Lu, Nanning City, Guangxi Province, ChinaChina Pos：530007

    Tel：（+86）-771-3243905;（+86）-771-3244920Email：nfnyxb@163.com

    Supported by: Beijing Renhe Information Technology Co., Ltd. 

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return