WANG Qian, YANG Bao, LI Yin, JIANG Qin-yang, HUANG Yan-na. 2024: Resveratrol inhibits lipid droplet formation in 3T3-L1 cells via ESR1-PI3K signaling pathway. Journal of Southern Agriculture, 55(7): 1916-1924. DOI: 10.3969/j.issn.2095-1191.2024.07.005
Citation: WANG Qian, YANG Bao, LI Yin, JIANG Qin-yang, HUANG Yan-na. 2024: Resveratrol inhibits lipid droplet formation in 3T3-L1 cells via ESR1-PI3K signaling pathway. Journal of Southern Agriculture, 55(7): 1916-1924. DOI: 10.3969/j.issn.2095-1191.2024.07.005

Resveratrol inhibits lipid droplet formation in 3T3-L1 cells via ESR1-PI3K signaling pathway

  • 【Objective】To investigate the effects of resveratrol(RES)and estrogen receptor 1(ESR1)on the formation of lipid droplets in 3T3-L1 cells and their regulatory mechanisms,and to provide theoretical basis for study of the potential mechanism of RES to inhibit fat deposition.【Method】Using 3T3-L1 cells as test materials,small interfering RNA (siRNA)targeting ESR1 gene was designed and synthesized. RES was added to 3T3-L1 cell culture medium,with the addition of equal amounts of dimethyl sulfoxide(DMSO)as control,the effects of RES and ESR1 on the differentiation of 3T3-L1 cells were analyzed using oil red O staining,real-time fluorescence quantitative PCR assay and Western blotting assay.【Result】Compared with the control group,RES significantly(P<0.05)reduced the production of lipid droplets in 3T3-L1 cells,extremely significantly(P<0.001)up-regulated the relative expression of ATGL,a key gene for lipolysis, and extremely significantly(P<0.001)down-regulated the relative expression of CEBPαPPARγ and FAS,key genes for adipogenesis. RES extremely significantly(P<0.001)increased the relative expression of ESR1 gene expression,and extremely significantly(P<0.01)increased the relative expression level of ESR1 protein;at the same time,RES extremely significantly(P<0.001)down-regulated the relative expression of PI3K and AKT genes in the PI3K signaling pathway, and extremely significantly(P<0.001)up-regulated the relative expression of FOXO1 gene. Interference with ESR1 gene extremely significantly(P<0.01)increased the generation of lipid droplets in 3T3-L1 cells,extremely significantly(P<0.001)down-regulated the relative expression of ATGL gene,and extremely significantly(P<0.001)up-regulated the relative expression of CEBPαPPARγ and FAS genes;after the addition of RES,the lipid droplets of 3T3-L1 cells interfering with ESR1 gene were not significantly different(P>0.05,the same below)compared with those without RES. There was no significant difference in the relative expression of ATGLCEBPαPPARγ and FAS genes. Interfering with ESR1 gene could extremely significantly(P<0.001)up-regulate the relative expression of PI3K and AKT genes in the PI3K signaling pathway,and extremely significantly(P<0.001)down-regulate the relative expression of FOXO1 gene; the relative expression of PI3KAKT and FOXO1 genes were not significantly different when RES was added compared with that of not adding RES.【Conclusion】Through the ESR1-PI3K signaling pathway,RES can up-regulate the relative expression of ATGL,the key gene of lipolysis,and down-regulate the relative expression of CEBPαPPARγ and FAS,the key genes of fat synthesis,thereby inhibiting the formation of lipid droplets in 3T3-L1 cells.
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