Regulation of compound preparation of Guiqi Yimu on oxidized lipids in blood of postpartum dairy cows

【Objective】To investigate the regulation of oxidized lipids in the blood of postpartum dairy cows by Guiqi yimu compound preparation, with a view to further clarifying the anti-inflammatory and antioxidant mechanisms of Guiqi Yimu compound preparation, and to provide reference basis for the application of Guiqi yimu compound preparation in the health care and disease control of postpartum dairy cows. 【Method】Fifteen healthy Holstein cows with similar body condition and 2-3 parities were selected and randomly divided into the control group(n=7) and the experimental group(n=8). The cows in the experimental group were dosed with Guiqi Yimu compound formulation 500 mL/cow after parturition for 6 consecutive d, while the cows in the control group were dosed with an equal volume of drinking water every day. Blood was collected from the tail vein before morning feeding 7 d postpartum and plasma samples were prepared. Ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) was used to tandem quantitatively detect the content of oxidized lipids in the plasma of two groups of dairy cows, analyze the changing law of the metabolic profile of oxidized lipids in the plasma of two groups of dairy cows, and screen for the differences of oxidized lipids in the blood of postpartum dairy cows and carry out the analysis of metabolic pathway enrichment. 【Result】A total of 44 oxidized lipids were identified in the plasma of 2 groups of cows, including 18 arachidonic acids and its metabolites, 12 linolenic acids and its derivatives, 9 eicosapentaenoic acids and its derivatives, 4 docosahexaenoic acids and its derivatives, and 1 docosapentaenoic acid and its derivatives. At 7 d postpartum, 8 oxidized lipid metabolic profiles of cows plasma in the experimental group changed significantly compared with the control group(P<0.05, the same below), of which 5 oxidized lipids molecules were significantly up-regulated(VIP≥1,FC≥2), including 12-HETE, 11-HETE, 15-HETE, 5-HETE and 15-oxoETE, and three were significantly down-regulated(VIP≥1,FC≤0.5), including 20-HETE, 8, 9-EET and TXB2. Pathway enrichment analysis showed that the differential lipid molecules were mainly enriched in arachidonic acid metabolic pathway, inflammatory mediator-regulated pathway of TRP channels and PPAR signaling pathway. 【Conclusion】Guiqi Yimu compound preparation mainly regulates lipid metabolism in postpartum cows through arachidonic acid metabolic pathway, inflammatory mediator regulation of TRP channels and PPAR signaling pathway, thus exerting anti-inflammatory and antioxidant effects.