Transcriptome analysis of Diaphorina citri Kuwayama in response to beta-cypermethrin stress

【Objective】 To screen Diaphorina citri Kuwayama resistance genes and their network regulation under the stress of beta-cypermethrin, and explore the resistance mechanism of D. citri to pyrethroid insecticides.【Method】 The Illumina NovaSeq 6000 sequencing system was used to sequence the transcriptome of the adult citrus psyllids treated with beta-cypermethrin and blank control. The differentially expressed genes(DEGs)were functionally annotated and classified using bioinformatics softwares such as HISAT2,DESeq2 and clusterProfiler,as well as the signal pathways analysis involved.【Result】 A total of 4596 significant DEGs genes were screened in the citrus psyllid transcriptome data,in which 2900 were significantly up-regulated and 1696 were significantly down-regulated. Among the DEGs,624 were enriched in 103 different metabolic pathways in KEGG. Seven enriched pathways of insect resistance mainly involved Hippo signaling pathway,ribosome,phagosome,drug metabolism,purine metabolism,thiamine metabolism,apoptosis,etc. Thirty-eight DEGs were related to insect drug resistance,involving multiple metabolic resistance and target resistance genes,including cytochrome oxidase,glutathione transferase,sodium ion channel,adenosine triphosphate synthase,cytochrome b,acid phosphoesterase,cadherin,glycosyltransferase,decarboxylase,ABC transporter,etc.【Conclusion】In this study,a lot of drug resistance-related genes of D. citri in response to pyrethroid insecticides are mined,and four significantly enriched metabolic pathways are closely related to insect resistance,such as Hippo signaling pathway,ribosome,phagosome,and thiamine metabolism,which provides a rich data basis for studying the drug resistance mechanism of citrus psyllid and for drug resistance management.