Transcriptomic analysis of ovary of Procambarus clarkii under sun-drying environmental stress

【Objective】The purpose of the study was to explore the gene expression changesin ovaries of Procambarus clarkii under different sun-drying stresses by transcriptomic sequencing technology, and to screen outthe candidate genes and signaling pathways related to ovarian development,so as toprovide a theoretical basis for studying the mechanism of sun-drying stress in promoting the ovarian development of P. clarkii.【Method】The female P. clarkii were sampled at 0 d(CK),3 d(T1),7 d(T2)and 10 d(T3)of sun-drying environmental stress,and ovary cDNA libraries were constructed for transcriptome sequencing,and the differentially expressed genes(DEGs)were subjected to GO functional annotation and KEGG signaling pathway enrichment analysis.【Result】A total of 86377 Unigenes were obtained. Of these, 22712 Unigenes were successfully annotated in Nr,KOG/COG, Swiss-Prot and KEGG databases. A total of 1115 DEGs were obtained between the stress group(T1,T2,T3)and the CK. The results of KEGG signaling pathway enrichment analysis showed that DEGs enriched on the pathways associated with ovarian development, including PI3K-Akt signaling pathway,GnRH signaling pathway and estrogen signaling pathway. The real-time fluorescence quantitative PCR verification results showed that the expression patterns of the three DEGs was basically consistent with the results of transcriptome analysis,proving that the transcriptome data were accurate and reliable. The ten DEGs related to ovarian development were screened out, including B2M, TUBA1B, MT-CYB, MT-ND1, MT-ND2, MT-ND4, DSX, Pck2, PIM3, and serine protease nudel-like. The expressions of these DEGs were analyzed, and the results showed that these genes regulated ovarian development during the period of sun-drying environmental stress.【Conclusion】 In this study,ten DEGs including B2M, TUBA1B,MT-CYB,MT-ND1,MT-ND2,MT-ND4, DSX,Pck2,PIM3 and serine protease nudel-like, and three enriched pathway sincluding PI3K-Akt signaling pathway, GnRH signaling pathway, and estrogen signaling pathway are involved in the regulation of ovarian development during the period of sun-drying environmental stress.