Screening and primary identification of Pisang Awak（ABB） mutant with resistance against Fusarium oxysporum f. sp. cubense and early flowering

【Objective】 To screen and identify the mutant 55b of Jinfen 1 with fusarium wilt resistance and early flowering，and to lay a foundation for breeding new varieties of Pisang Awak（ABB）with fusarium wilt resistance by using the mutant strains.【Method】The resistance and early flowering mutant of Jinfen 1（55b）was gradually separated by tissue culture combined with field screening，and its morphological and biological characteristics were identified in the field. At the same time， disease observation and seedling resistance were carried out in the field， and genetic analysis was conducted on the genomic DNA of the mutant 55b and wild-type Jinfen 1 using ISSR molecular markers.【Result】 The growth of the mutant strain 55b was moderate. The average height of the pseudostem was 335.3 cm，and the base circumference and middle circumference of the pseudostem were 84.2 and 57.5 cm，respectively，which were 27.1%，23.3% and 11.5% smaller than that of Jinfen 1. The growth cycle of new planting 55b was about 2-3 months shorter than that of Jinfen 1， and that of perennial banana was about 1-2 months shorter than that of Jinfen 1. The incidence of fusarium wilt in the field of 55b was much lower than that of Jinfen 1， and the incidence of 55b in the banana garden infected with Foc1 physiological species（Fusarium oxysporum f. sp.cubense race1）was under 10.0%，and the incidence of Jinfen 1 was 30.0%-100.0%. After 80 d of inoculation treatment at root，the incidence rate of Jinfen 1 seedlings was 95.6% and the mortality rate was 75.0%，while the incidence rate of 55b was 6.5% with slight yellowing. Fruit quality and yield per unit area of 55b were not greatly different from that of Jinfen 1. The polymorphism ratio between the mutant strain 55b and Jinfen 1 was 7.7%， the genetic similarity coefficient was 0.96，and the genetic background was similar.【Conclusion】 The fusarium wilt resistant，early maturing and dwarfing strains isolated and screened from the offspring of Jinfen 1 mutant 55b can be used to breed new varieties of Pisang Awak（ABB）that can be widely planted，or the parent breeding materials derived from them can play a role in the research of banana breeding.