Resistance evaluation of grape germplasm resources to Meloidogyne vitis

【Objective】 Resistance to Meloidogyne vitis of grape germplasms were evaluated to provide reference for selecting M. vitis-resistant germplasm materials, arranging M. vitis-resistant varieties, and developing green prevention and control technology.【Method】 Inoculating grape germplasms seedlings with second-stage larvae to determine M. vitis-resistance evaluation indexes(gall index, egg granule index, disease index and reproductive frequency) of 20 materials. Taking Red Globe as control materials, comprehensive evaluation was conducted through cluster analysis and subordinate function operation method.【Result】 Gall index(2.95-15.97), egg granule index(0.77-10.14) and disease index(46.70-93.30) of the 19 grape germplasm materials were lower than those of Red Globe, the control. Reproductive frequency(0.06-0.92) of 18 materials except for Pearl of Csaba were lower than that of Red Globe. Pearl of Csaba and Pinot Noir enjoyed 4 higher resistance indexes except for gall index, without significant difference of the other 3 resistance indexes from the control(P>0.05), indicating they were highly susceptible varieties, and the variation trend of different resistance indexes in other germplasms was not consistent. Taking 4 resistance indexes as parameters, the M. vitis-resistance levels of the 20 grape materials were divided into 3 categories by systematic cluster analysis and subordinate function operation methods. Gold Finger, Fujiminori, Golden Rose, Red Ruby, Manicure Finger, Beauty Seedless, Witch Fingers, Canadice, Muscat Hamburg, Summer Black, Himrod were mediumly M. vitis-resistant;Longyan, Purple Sweet Seedless, Crimson, Fantasy, Kyoho, Victoria were sensitive materials;Pearl of Csaba, Pinot Noir and Red Globe were highly sensitive materials.【Conclusion】 Among the 20 grape germplasm materials, 11 are mediumly-resistant materials, 6 are sensitive materials and 3 are highly sensitive materials. No high resistance germplasms or immune germplasms are found in tested materials, and Himrod has the strongest resistance and the Pearl of Csaba the lowest.