Comparison of flavonoids content,polyphenols content and antioxidant activity of different parts of Clausena lansium （Lour.） Skeels and C. anisum-olens（Blanco） Merr.

【Objective】To compare flavonoids content,polyphenols content and antioxidant activities of different cultivars and parts of 5 cultivars of Clausena lansium(Lour.)Skeels and C. anisum-olens(Blanco)Merr.,so as to provide theoretical basis for development and utilization of the plant resources of Clausena.【Method】Xiangmi C. lansium,Jixin C.lansium,Longzhou 1 C. anisum-olens,YYS-002 and Longzhou single-stone C. anisum-olens were used as test materials.In leaf,stem,stone and peels of materials,total flavonoids content was determined by NaNO₂-AlCl₃-NaOH method,and total polyphenols content was determined by Folin-Ciocalteu method. The antioxidant activity were evaluated by 1,1-diphenyl-2-picrylhydrazyl radical 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl free radical(DPPH·)clearance rate,hydroxyl free radical(·OH)clearance rate and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)free radical(ABTS⁺)clearance rate. Correlations between total polyphenols content,total flavonoids content and antioxidant activity indexes were analyzed by Pearson correlation analysis method.【Result】Total flavonoids content in leaf,stem,stone and peel of the 5 cultivars ranged from 0.34 to 86.23 mg/g and total polyphenols content 0.54 to 43.33 mg/g. Total flavonoids content and total polyphenols content in C. lansium were generally lower than those of C. anisum-olens. The order of total flavonoids content and total polyphenols content in different parts was as follows:peel>leaf>stem>stone. By comprehensive comparison,total flavonoids content and total polyphenols content in different parts of Longzhou single-stone C. anisum-olens were higher than those in other varieties,while they were significantly higher in peel and leaf than in other parts(P<0.05). The results of antioxidant activity experiments showed that the peel extract of Longzhou single-stone C. anisum-olens had the highest DPPH·,·OH and ABTS+clearance rate,which were 96.01%,92.23% and 76.96%,equivalent to 0.03,0.03,0.01 mg/g Vitamin C(Vc)clearance. The clearance rate 50(IC₅₀)values were 0.33,0.45 and 0.52 mg/mL,respectively. Antioxidant activity of different parts was also shown as:peel>leaf>stem>stone. Correlation analysis indicated that total flavonoids content and total polyphenols content were significant positively correlated with each index of antioxidant activities(P<0.01).【Conclusion】The peel of Longzhou single-stone C. anisum-olens has the highest antioxidant capacity,which can be used as a natural antioxidant resource for in-depth research and development. Flavonoids and polyphenols are the related substances for the antioxidant activities of C. lansium and C. anisum-olens,while polyphenols significantly influence antioxidant activities.