Comprehensive regulation of sperm motility of Rutilus rutilus lacustris by Na⁺,K⁺,pH,and 3 monosaccharides

【Objective】 To clarify the individual and comprehensive regulation of sperm motility of Rutilus rutilus lacustris by Na⁺，K⁺，pH and 3 monosaccharides，to screen the suitable formula of the sperm activating liquid of R.rutilus lacustris，so as to provide technical reference for artificial reproduction and genetic resource conservation of the R.rutilus lacustris.【Method】 Fast movement time（FT）and life time（LT）were taken as indicators，and single factor test and orthogonal test were used to study individual and comprehensive regulation effects of Na⁺，K⁺，pH and 3 monosaccharides（glucose，fructose and galactose）on sperm motility of the R.rutilus lacustris under different osmotic pressure conditions to form a suitable formula of the sperm activating liquid of R.rutilus lacustris.【Result】 When Na⁺ solution osmotic pressure was 282.36 kPa，the FT and LT of R.rutilus lacustris sperm reached the maximum（40.00 and 285.33 s）.When K⁺ solution osmotic pressure was 211.77 kPa，the FT and LT of R.rutilus lacustris sperm reached the maximum（36.00 and 130.33 s）.When glucose solution osmotic pressure was 352.95 kPa，the FT and LT of R.rutilus lacustris sperm reached the maximum（43.67 and 355.33 s）.When fructose solution osmotic pressure was 352.95 kPa，the FT and LT of R.rutilus lacustris sperm reached the maximum（45.00 and 372.67 s）.When galactose solution osmotic pressure was 235.30 kPa，the FT and LT of R.rutilus lacustris sperm reached the maximum（40.00 and 357.00 s）.When pH was 8.0，the FT and LT of R.rutilus lacustris sperm reached the maximum（30.00 and 100.33 s）.Orthogonal test results showed that the optimal formula for comprehensive regulation of sperm motility of the R.rutilus lacustris was： Fructose solution osmotic pressure of 117.65 kPa，pH of 8.0，Na⁺ solution osmotic pressure of 188.24 kPa，FT of 58.33 s and LT of 370.00 s.The regulation intensity was fructose>Na⁺>pH.The results showed that fructose and Na+had significant effects on FT and LT regulation of R.rutilus lacustris sperm（P<0.05），but the effect of pH was not significant（P>0.05）.【Conclusion】 Appropriate concentrations of Na⁺，K⁺，glucose，fructose and galactose promotes sperm motility of the R.rutilus lacustris mainly through changing osmotic pressure.The suitable pH is 7.5-8.5.Through orthogonal test，the optimal activating formula of the R.rutilus lacustris sperm is： Fructose of 117.65 kPa，pH of 8.0，and Na⁺ of 188.24 kPa，and regulation effect of this formula is better than that of the single-factor optimal osmotic pressure.The regulation intensity is： fructose>Na⁺>pH.