Polymorphism of ARHGEF37 gene and its effect on growth traits in F₁ generation pigs of（Duroc×Saba）♂×Yorkshire×（Landrace×Saba）♀

【Objective】To reveal the effect of ARHGEF37 gene polymorphisms on the growth traits of pigs,so as to provide a reference for further breeding,development and the utilization of Diansa pig matching line with this gene marker.【Method】The study used 274 F₁ generation pigs(Duroc×Saba)♂×Yorkshire×(Landrace×Saba)♀(hereafter DS♂×YLS♀)to screen for polymorphisms in the ARHGEF37 gene by the direct sequencing of PCR amplified products. The effects of genotypes and haplotype combinations of each SNP locus on five growth traits were analyzed by the least square model.【Result】It was found that 4 SNPs loci were located on exon 8 of ARHGEF37 gene:C1262G,T1293C,G1395A and C1398T The frequency of CC,TT,GA and CC genotype was the highest,followed by allele C,T,G,C and haplotype CTGC.Except for the T1293C locus,the remaining showed a heterozygosity between 0.4552-0.4940,indicating more abundant genetic diversity. The age to 100 kg body weight in the C1262G GG genotype(258.93±2.95 d)was significantly higher than that in the CG genotype(249.87±2.68 d)(P<0.05,the same below). Measurements of the average daily weight gain in pigs of 30-60 kg showed that the CG genotype(462.44±15.44 g)was significantly higher than that of the CC genotype(409.29±14.30 g)and the TT genotype(445.04±9.70 g)at T1293C was extremely significantly higher than that of the TC genotype(329.33 ± 42.26 g)(P<0.01). The average daily weight gain in pigs between 30-100 kg in the TT genotype(516.96±6.44 g)was significantly higher than that of the TC and CC genotypes(470.92±28.08 g and 480.64±16.94 g,respectively). In addition,the time to achieve 60 and 100 kg body weight was the shortest in the CTGC/GTGC combination(161.84±5.07 d and 241.00±5.11 d)and the longest in the CTGC/CCGC combination(188.80±11.33 d and 272.60±11.42 d).The average daily gains between 30-60 kg and between 30-100 kg were the highest in the CTGC/GTAT and CTGC/GTGC combinations(479.56 ± 20.66 g and 545.42 ± 19.76 g,respectively),and the lowest in the CTAC/CCGC combination(275.00±84.33 g and 437.00±55.88 g).【Conclusion】The genetic diversity of G1395A and C1398T of ARHGEF37 gene is more abundant. The CG genotype of the C1262G locus,TT genotype of the T1293C locus and the combination of CTGC/GTGC haplotype have more influence on the growth traits of the F₁ generation of DS♂ ×YLS♀ pigs. The growth traits of individuals carrying the CTGC haplotype are all at a relatively high level.