Cloning and bioinformatics analysis of gibberellin insensitive dwarf1 gene DbGID1s in Dioscorea bulbiferas

【Objective】 The aim of this study was to clone gibberellin insensitive dwarf1 (GID1) gene DbGID1s in Dioscorea bulbifera, conduct bioinformatics analysis, and to provide a theoretical reference for further exploring the mechanism of DbGID1s protein in the growth and development of D. bulbifera.【Method】 DbGID1s were cloned by RT-PCR method; the physical and chemical properties, domains, phosphorylation sites and phylogenetic evolution of these genes were pridicted with bioinformatics softwares.【Result】 Four s equences of gene DbGID1A were obtained, namely DbGID1A(GenBank accession number:MT648372), DbGID1B1(GenBank accession number:MT648374), DbGID1B2 (GenBank accession number:MT648375) and DbGID1C(GenBank accession number:MT648373). The lengths of four nucleic acid sequences were 862, 1273, 1081 and 1164 bp, respectively, and encoding 292, 340, 289 and 360 amino acids residues, respectively. The molecular weights of the four DbGID1s proteins were 32079.17-40301.83 Da, and their theoretical isoelectric points(pI) varied from 6.05 to 8.62. They were unstable, hydrophilic proteins and located on external membrane. No signal peptide was found in the four DbGID1s proteins. Their phosphorylation sites were mainly serine (Ser, S), and the phosphorylation sites of the four proteins had coincidence sites and mutation sites. The four proteins not only had the hydrolytic activite area of α/β folding hydrolase superfamily carboxylic acid lipase, but also had conserved domains(HGG and GXSXG) and catalytic joint sites(S, D and H) of hormone lipase(HSL). The similarity of proteins between D. bulbifera and other species was high, and the homology between D. bulbifera and yam was over 90%.【Conclusion】 The DbGID1s gene in D. bulbifera has the basic characteristics of GID1 genes in many plants and the encoded proteins have the typical domain of GID1 protein, so it is possible that DbGID1s genes participate in gibberellin signal transduction and regulate the growth and development of organs of D. bulbifera.