Analysis of bioinformatics and gene expression of glycosyltransferase gene family in Fragaria vesca

【Objective】In this study, bioinformatics analysis and gene expression analysis of Fragaria vesca glycosyltransferase gene(FvUGT) family were carried out to provide theoretical basis for the FvUGT gene function of F. vesca and the exploration of UGT regulation of fruit development and anthocyanin and quality formation of F. vesca.【Method】Based on the Phytozome database, 138 FvUGT family genes were identified, bioinformatics methods were used to analyze the protein properties, gene structure, conserved domains and evolutionary relationships of these FvUGT genes, and using real-time fluorescence quantitative PCR method to analyze the expression profiles of UFGT candidate genes in various tissues(root, petiole, leaf and fruit) of F. vesca(red fruit and white fruit).【Result】The FvUGT gene family could be divided into 12 subfamilies(A, C, D, E, F, G, H, I, J, K, L and N), each of which contained 16, 2, 23, 32, 8, 1, 5, 5, 7, 3, 21 and 4 members. Chromosomal localization analysis revealed that the coding genes of 138 FvUGT genefamily members were distributed on all chromosomes except chromosome 2, and the distribution was uneven. The FvUGT gene intron length, exon position and number were different among different members, and the FvUGT family had a strong differentiation in the process of evolution. qRT-PCR found that the FvUFGT70 gene was highly expressed in leaves and petioles. The FvUFGT94 gene was expressed in various tissues;FvUFGT67 and FvUFGT68 genes had higher expression in roots;FvUFGT95 and FvUFGT96 genes had expression in fruits, which was significantly higher than that of other tissues(P<0.05, the same below). At the fruit's small green stage, turning stage and ripe stage, and the expression levels of the two genes FvUFGT33 and FvUFGT95 in F. vesca red fruit were significantly higher than white fruit during the ripe stage.【Conclusion】The 7 FvUFGT genes show obvious tissue differences, and FvUFGT33 and FvUFGT95 have high expression in fruit, and the expression in red fruit type is significantly higher than white fruit type. It is speculated that Fv UFGT33 and FvUFGT95 play a role in the synthesis of anthocyanins.