LI Hao, CHEN Ya-ping, LU Zhi-hui, GUO Jian-yang, LI Ya-hong, ZHU Lin-yun, HE Shu-qi, GUI Fu-rong. 2021: Response comparison of protective and detoxification enzymes in Spodoptera frugiperda(J. E. Smith) and Spodoptera litura(Fabricius) larvae to two insecticides. Journal of Southern Agriculture, 52(3): 559-569. DOI: 10.3969/j.issn.2095-1191.2021.03.002
Citation: LI Hao, CHEN Ya-ping, LU Zhi-hui, GUO Jian-yang, LI Ya-hong, ZHU Lin-yun, HE Shu-qi, GUI Fu-rong. 2021: Response comparison of protective and detoxification enzymes in Spodoptera frugiperda(J. E. Smith) and Spodoptera litura(Fabricius) larvae to two insecticides. Journal of Southern Agriculture, 52(3): 559-569. DOI: 10.3969/j.issn.2095-1191.2021.03.002

Response comparison of protective and detoxification enzymes in Spodoptera frugiperda(J. E. Smith) and Spodoptera litura(Fabricius) larvae to two insecticides

Funds: 

National Key Research and Development Program of China(2019YFD0300101, 2019YFD0300-104)

More Information
  • Received Date: March 03, 2021
  • 【Objective】The research aimed to clarify the response of different insecticides on the activities of protective enzymes and detoxification enzymes in the larvae of Spodoptera frugiperda(J. E. Smith) and Spodoptera litura(Fabricius) to two insecticides, and to provide theoretical basis for further study on the field control and and insecticide resistance mechanism of S. frugiperda and S. litura.【Method】The toxicity of emamectin and chlorantraniliprole to the 3rd instar larvae of S. frugiperda and S. litura were assayed by leaf-dipping method by feeding wheat leaves treated with sublethal concentration(LC25) and median lethal concentration(LC50) of the pesticides respectively, and the activities of protective enzymes[superoxide dismutase(SOD), peroxidase(POD) and catalase(CAT)]and detoxification enzymes[glutathione S-transferase(GST), cytochrome P450(CYP450) and carboxylesterase(CarE)]in larvae after being treated for 6, 12, 18, 24 and 48 h were measured and analyzed.【Result】The toxicity of emamectin to S. frugiperda and S. litura was higher than that of chlorantraniliprole. The activity of CYP450 in both pests increased after the two insecticide treatments, and was significantly higher in S. frugiperda than that in S. litura. The CYP450 activity in S. frugiperda was more active at low concentration(LC25) of emamectin and chlorantraniliprole than at high concentration(LC50), which was the highest at 48 h as 15.72±0.41(emamectin) and 15.36±0.47 ng/mL(cholrantraniliprole). However, the activities of two detoxification enzymes GST and CarE in S. frugiperda were lower than those in S. litura after emamectin treatment. The activities of protective enzymes SOD and POD in both pests decreased after treated with emamectin, and were lower in S. frugiperda than those in S. litura;while the activities of SOD and POD in S. frugiperda increased after treated with chlorantraniliprole. The activities of SOD and CAT were higher in both pests at LC50 chlorantraniliprole than at LC25, and were higher in S. frugiperda than in S. litura. The response activities of protective enzymes and detoxification enzymes in both pests were greatly affected by the two insecticides, and species, insecticide, concentration, time, species×concentration and insecticide×concentration had extremely significant effects on the activities of protective enzymes and detoxification enzymes(P<0.01). There was a strong correlation between the activities of SOD and CAT of both S. frugiperda and S. litura under two insecticides treatments.【Conclusion】The susceptibility of S. frugiperda to emamectinis higher than that of S. litura, while the sensitivity to chlorantraniliprole is lower than that of S. litura, and the two pests enhance detoxification metabolism of insecticides by increasing CYP450 activity. In agricultural production, it is recommended to use emamectin as an agent for controlling S. frugiperda.
  • 陈羿渠, 向兴, 贡常委, 王学贵.2017.氯虫苯甲酰胺亚致死剂量对甜菜夜蛾主要解毒酶活性与生长繁殖的影响[J].中国农业科学, 50(8):1440-1451.doi:10.3864/j.issn.0578-1752.2017.08.008.[Chen Y Q, Xiang X, Gong C W, Wang X G.2017.Effects of sublethal doses of chloran-traniliprole on the detoxification enzymes activities and the growth and reproduction of Spodoptera exigua[J].Scientia Agricultura Sinica, 50(8):1440-1451.]
    戴瀚洋, 孙洋, 柏立新, 赵静, 肖留斌, 谭永安.2015.亚致死浓度甲维盐胁迫对甜菜夜蛾幼虫解毒酶系活力及其相关基因表达量的影响[J].棉花学报, 27(2):149-158.doi:10.11963/issn.1002-7807.201502008.[Dai H Y, Sun Y, Bai L X, Zhao J, Xiao L B, Tan Y A.2015.Activities of detoxification enzymes and expressions of related genes in Spodoptera exigua larvae treated with sublethal con-centrations of emamectin benzoate[J].Cotton Science, 27(2):149-158.]
    高祖鹏, 郭井菲, 何康来, 王振营.2020.乙基多杀菌素对草地贪夜蛾幼虫的毒力及对其解毒酶和乙酰胆碱酯酶活性的影响[J].害虫学报, 63(5):558-564.doi:10.16380/j.kcxb.2020.05.004.[Gao Z P, Guo J F, He K L, Wang Z Y.2020.Toxicity of spinetoram and its effects on the de-toxifying enzyme and acetyl cholinesterase activities in Spodoptera frugiperda(Lepidoptera:Noctuidae) larvae[J].Acta Entomologica Sinica, 63(5):558-564.]
    何超, 沈登荣, 尹立红, 张睿, 袁盛勇, 田学军.2020.3种杀虫剂对井上蛀果斑螟存活、化蛹及羽化的影响[J].河南农业科学, 49(9):105-111.doi:10.15933/j.cnki.1004-3268.2020.09.013.[He C, Shen D R, Yin L H, Zhang R, Yuan S Y, Tian X J.2020.Effects of three insecticides on sur-vival, pupation and emergence of Assara inouei Yamanaka[J].Journal of Henan Agricultural Sciences, 49(9):105-111.]
    黄训兵, 李辉, 涂雄兵, 张泽华.2021.四种植物源化合物对亚洲小车蝗存活率、解毒酶和保护酶活性的影响[J].植物保护学报, 48(1):158-164.doi:10.13802/j.cnki.zwbhxb.2021.2021821.[Huang X B, Li H, Tu X B, Zhang Z H.2021.Effects of four plant-derived compounds on the survival rate and activities of detoxification enzymes and protective enzymes in the grasshopper Oedaleus asiaticus[J].Journal of Plant Protection, 48(1):158-164.]
    蒋兴川, 沈怿丹, 孙劲超, 李秀霞, 黄勇, 董永成, 操海群.2019.氯虫苯甲酰胺和甲维盐对草地贪夜蛾幼虫的毒力及解毒酶活性的影响[J].环境害虫学报, 41(5):961-967.doi:10.3969/j.issn.1674-0858.2019.05.5.[Jiang X C, Shen Y D, Sun J C, Li X X, Huang Y, Dong Y C, Cao H Q.2019.Effect of chlorantraniliprole and emamectin benzoa-te on toxicity and detoxification enzymes activity in Spodoptera frugiperda larva[J].Journal of Environmental En-tomology, 41(5):961-967.]
    李长春, 宁青, 戴余军, 王立华, 李国元, 彭宇.2019.拟环纹豹蛛谷胱甘肽S-转移酶基因的克隆及表达分析[J].江苏农业学报, 35(5):1068-1074.doi:10.3969/j.issn.1000-4440.2019.05.010.[Li C C, Ning Q, Dai Y J, Wang L H, Li G Y, Peng Y.2019.Cloning and expression analysis of glu-tathione S-transferase gene in Pardosa pseudoannulata[J].Jiangsu Journal of Agricultural Sciences, 35(5):1068-1074.]
    鲁智慧, 和淑琪, 郭晋, 陈亚平, 胡雪琼, 李浩, 谢琦, 桂富荣.2020.取食不同寄主植物对草地贪夜蛾幼虫体内3类酶活性的影响[J].南方农业学报, 51(10):2461-2469.doi:10.3969/j.issn.2095-1191.2020.10.018.[Lu Z H, He S Q, Guo J, Chen Y P, Hu X Q, Li H, Xie Q, Gui F R.2020.Effects of host plants on activities of three groups of en-zymes in Spodoptera frugiperda(Smith) larvae[J].Jour-nal of Southern Agriculture, 51(10):2461-2469]
    欧善生, 梁沛, 宋敦伦, 史雪岩, 高希武.2012.氯虫苯甲酰胺亚致死剂量对棉铃虫生长发育和解毒酶活性的影响[J].植物保护, 38(4):1-8.doi:10.3969/j.issn.0529-1542.2012.04.001.[Ou S S, Liang P, Song D L, Shi X Y, Gao X W.2012.Effects of sublethal dosage of chlorantraniliprole on development and detoxifying enzymes activity of Helicoverpa armigera[J].Plant Protection, 38(4):1-8.]
    任娜娜, 谢苗, 尤燕春, 尤民生.2014.羧酸酯酶及其介导害虫抗药性的研究进展[J].福建农林大学学报(自然科学版), 43(4):337-344.doi:10.13323/j.cnki.j.fafu(nat.sci.).2014.04.005.[Ren N N, Xie M, You Y C, You M S.2014.An overview on the study of insect carboxylesterases(COEs) and the COE-mediated resistance to insecticides[J].Journal of Fujian Agriculture and Forestry University(Natural Science Edition), 43(4):337-344.]
    宋洁蕾, 李艳丽, 李亚红, 黄碧芬, 康云昌, 马庭矗, 张澍雷, 桂富荣.2019.不同杀虫剂对草地贪夜蛾的室内毒杀效果及毒力测定[J].南方农业学报, 50(7):1489-1495.doi:10.3969/j.issn.2095-1191.2019.07.12.[Song J L, Li Y L, Li Y H, Huang B F, Kang Y C, Ma T C, Zhang S L, Gui F R.2019.Measurements of indoor toxic effects and viru-lence of different insecticides against Spodoptera frugiperda[J].Journal of Southern Agriculture, 50(7):1489-1495.]
    唐芬芬, 杨伟克, 朱峰, 邵榆岚, 张永红, 白兴荣.2019.BmN-PV对家蚕抗氧化酶基因表达及其酶活性的影响[J].南方农业学报, 50(10):2308-2313.doi:10.3969/j.issn.2095-1191.2019.10.22.[Tang F F, Yang W K, Zhu F, Shao Y L, Zhang Y H, Bai X R.2019.Effects of BmNPV on the antioxidant enzyme gene expression and enzyme activity of Bombyx mori[J].Journal of Southern Agriculture, 50(10):2308-2313.]
    王芹芹, 崔丽, 王立, 黄伟玲, 代黎明, 袁会珠, 芮昌辉.2020.茚虫威对草地贪夜蛾的毒力及解毒酶的诱导作用[J].植物保护, 46(1):78-81.doi:10.16688/j.zwbh.2019557.[Wang Q Q, Cui L, Wang L, Huang W L, Dai L M, Yuan H Z, Rui C H.2020.Toxicity of indoxacarb to Spodoptera frugiperda and induction of its detoxifying enzymes[J].Plant Protection, 46(1):78-81.]
    邢静, 梁沛, 高希武.2011.亚致死浓度氯虫苯甲酰胺对小菜蛾药剂敏感度和解毒酶活性的影响[J].农药学学报, 13(5):464-470.doi:10.3969/j.issn.1008-7303.2011.05.05.[Xing J, Liang P, Gao X W.2011.Effects of sublethal concentrations of chlorantraniliprole on insecticide sus-ceptibility and detoxifying enzyme activity in Plutella xylostella[J].Chinese Journal of Pesticide Science, 13(5):464-470.]
    赵胜园, 罗倩明, 孙小旭, 杨现明, 姜玉英, 吴孔明.2019a.草地贪夜蛾与斜纹夜蛾的形态特征和生物学习性比较[J].中国植保导刊, 39(5):26-35.doi:10.3969/j.issn.1672-6820.2019.05.005.[Zhao S Y, Luo Q M, Sun X X, Yang X M, Jiang Y Y, Wu K M.2019a.Comparison of morpho-logical and biological characteristics between Spodoptera frugiperda and Spodoptera litura[J].China Plant Protec-tion, 39(5):26-35.]
    赵胜园, 孙小旭, 张浩文, 杨现明, 吴孔明.2019b.常用化学杀虫剂对草地贪夜蛾防效的室内测定[J].植物保护, 45(3):10-14.doi:10.16688/j.zwbh.2019160.[Zhao S Y, Sun X X, Zhang H W, Yang X M, Wu K M.2019b.Labo-ratory test on the control efficacy of common chemical insecticides against Spodoptera frugiperda[J].Plant Pro-tection, 45(3):10-14.]
    张百重, 苏栩, 卢留洋, 甄丛爱, 朱斌, 李亚设, 董文阳, 汪耿, 胥燕博, 孔凡彬, 刘润强, 陈锡岭, 高希武.2020.三种杀虫剂亚致死剂量对草地贪夜蛾细胞色素P450基因表达的影响[J].害虫学报, 63(5):565-573.doi:10.16380/j.kcxb.2020.05.005.[Zhang B C, Su X, Lu L Y, Zhen C A, Zhu B, Li Y S, Dong W Y, Wang G, Xu Y B, Kong F B, Liu R Q, Chen X L, Gao X W.2020.Effects of three insecticides at the sublethal dose on the expression of cy-tochrome P450 genes in Spodoptera frugiperda(Lepidop-tera:Noctuidae)[J].Acta Entomologica Sinica, 63(5):565-573.]
    张文成, 王开运, 牛芳, 王东, 任学祥.2009.虫螨腈胁迫对甜菜夜蛾保护酶系和解毒酶系的诱导效应[J].植物保护学报, 36(5):455-460.doi:10.13802/j.cnki.zwbhxb.2009.05.005.[Zhang W C, Wang K Y, Niu F, Wang D, Ren X X.2009.Induction of chlorfenapyr on endogenous pro-tective and detoxifying enzymes in beet armyworm, Spodoptera exigua(Hübner)[J].Journal of Plant Protection, 36(5):455-460.]
    张振威, 赵清泉, 郝昕, 潘佳亮, 马玲.2019.阿维菌素和甲维盐对舞毒蛾幼虫的毒力及解毒酶活性的影响[J].东北林业大学学报, 47(5):118-122.doi:10.13759/j.cnki.dlxb.2019.05.023.[Zhang Z W, Zhao Q Q, Hao X, Pan J L, Ma L.2019.Effect of avermectin and emamectin benzoa-te on toxicity and detoxifying enzymes activity in Lymantria dispar larva[J].Journal of Northeast Forestry Univer-sity, 47(5):118-122.]
    Bashan N, Kovsan J, Kachko I, Ovadia H, Rudich A.2009.Positive and negative regulation of insulin signaling by reactive oxygen and nitrogen species[J].Physiological Re-views, 89(1):27-71.doi: 10.1152/physrev.00014.2008.
    Carvalho R A, Omoto C, Field L M, Williamson M S, Bass C.2013.Investigating the molecular mechanisms of organo-phosphate and pyrethroid resistance in the fall armyworm Spodoptera frugiperda[J].PLoS One, 8(4):e62268.doi: 10.1371/journal.pone.0062268.
    Goergen G, Kumar P L, Sankung S B, Togola A, Tamò M.2016.First report of outbreaks of the fall armyworm Spodoptera frugiperda(J.E.Smith) (Lepidoptera, Noctui-dae), a new alien invasive pest in west and central Africa[J].PLoS One, 11(10):e0165632.doi: 10.1371/journal.pone.0165632.
    Gui F R, Lan T M, Zhao Y, Guo W, Dong Y, Fang D M, Liu H, Li H M, Wang H L, Hao R S, Cheng X F, Li Y H, Yang P C, Sahu S K, Chen Y P, Cheng L, He S Q, Liu P, Fan G Y, Lu H R, Hu G H, Dong W, Chen B, Jiang Y, Zhang Y W, Xu H H, Lin F, Slipper B, Postma A, Jack-son M, Abate B A, Tesfaye K, Demie A L, Bayeleygne M D, Degefu D T, Chen F, Kuria P K, Kinyua Z M, Liu T X, Yang H M, Huang F N, Liu X, Sheng J, Kang L.2020.Genomic and transcriptomic analysis unveils popu-lation evolution and development of pesticide resistance in fall armyworm Spodoptera frugiperda[J].Protein & Cell.doi: 10.1007/s13238-020-00795-7.
    Montezano D G, Sosa-Gómez D R, Specht A, Roque-Specht V F, Sousa-Silva J C, Paula-Moraes S V, Peterson J A, Hunt T E.2018.Host plants of Spodoptera frugiperda(Lepidoptera:Noctuidae) in the Americas[J].African En-tomology, 26(2):286-300.doi: 10.4001/003.026.0286.
    Okuma D M, Bernardi D, Horikoshi R J, Bernardi O, Silva A P, Omoto C.2018.Inheritance and fitness costs of Spodoptera frugiperda(Lepidoptera:Noctuidae) resistance to spi-nosad in Brazil[J].Pest Management Science, 74(6):1441-1448.doi: 10.1002/ps.4829.
    Riveron J M, Irving H, Ndula M, Barnes K G, Ibrahim S S, Paine M J I, Wondji C S.2013.Directionally selected cy-tochrome P450 alleles are driving the spread of pyre-throid resistance in the major malaria vector Anopheles funestus[J].Proceedings of the National Academy of Scien-ces of the United States of America, 110(1):252-257.doi: 10.1073/pnas.1216705110.
    Senthil-Nathan S.2013.Physiological and biochemical effect of neem and other Meliaceae plants secondary metabo-lites against Lepidopteran insects[J].Frontiers in Physio-logy, 4:359.doi: 10.3389/fphys.2013.00359.
    Song X, Pei L, Zhang Y, Chen X, Zhong Q, Ji Y, Tang J, Feng F, Li B.2020.Functional diversification of three delta-class glutathione S-transferases involved in develop-ment and detoxification in Tribolium castaneum[J].In-sect Molecular Biology, 29(3):320-336.doi: 10.1111imb.12637.
    Teixeira L A, Andaloro J T.2013.Diamide insecticides:Global efforts to address insect resistance stewardship challenges[J].Pesticide Biochemistry and Physiology, 106(3):76-78.doi: 10.1016/j.pestbp.2013.01.010.
    Zhang B Z, Su X, Zhen C A, Lu L Y, Li Y S, Ge X, Chen D M, Pei Z, Shi M W, Chen X L.2020.Silencing of cyto-chrome P450 in Spodoptera frugiperda(Lepidoptera:Noc-tuidae) by RNA interference enhances susceptibility to chlorantraniliprole[J].Journal of Insect Science, 20(3):12;1-7.doi: 10.1093/jisesa/ieaa047.
    Zhang X L, Liao X, Mao K K, Yang P, Li D Y, Alia E, Wan H, Li J H.2017.The role of detoxifying enzymes in field-evolved resistance to nitenpyram in the brown plan-thopper Nilaparvata lugens in China[J].Crop Protection, 94:106-114.doi: 10.1016/j.cropro.2016.12.022.
  • Related Articles

  • Cited by

    Periodical cited type(1)

    1. 马其红,宋昌朔,石岩,杨春红,张彬. 黄粉虫蛹与葡萄糖饲喂条件下益蝽种群生命表的构建. 青岛农业大学学报(自然科学版). 2024(02): 95-101 .

    Other cited types(1)

Catalog

    Article Metrics

    Article views (156) PDF downloads (0) Cited by(2)
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return