Protective effects of geniposide on hepatotoxicity induced by bisphenol A in common carp(Cyprinus carpio)

【Objective】Explored the effects of different concentrations of geniposide on the hepatotoxicity of bisphenol A(BPA) in common carp(Cyprinus carpio), and provided a reference for screening hepatoprotective drug in fish.【Method】In this study, 75 common carps were divided into five treatment groups:blank control group, model group(0.5 mg/L BPA exposure) and geniposide treatment group(BPA+geniposide).Geniposide was set at low(1.25 g/kg), medium(2.50 g/kg) and high(5.00 g/kg) concentrations for 70 d.Then detected glutamic-pyruvic transaminase(GPT), glutamic oxalacetic transaminase(GOT), alkaline phosphatase(AKP), triglycerides(TG), total cholesterol(TC), high-density lipoprotein cholesterol(HDL-C), low-density lipoprotein cholesterol(LDL-C) levels in the serum, super oxide dismutase(SOD), catalase(CAT), glutathione(GSH), malondialdehyde(MDA) and total antioxidant capacity(T-AOC) levels, as well as the expression levels of fatp1, fas, ppar-α, nrf2, keap1, ho-1, tlr1, myd88, il-1β, il-6 and il-10 genes in the liver to evaluate the therapeutic effects of geniposide on hepatotoxicity in common carp.【Result】Compared with the control group, the specific growth rate of common carp in the model group was significantly reduced, and the feed coefficient was significantly increased(P< 0.05, the same below).The combined feeding of three concentrations of geniposide could significantly increase the specific growth rate, reduce the feedcoefficient, and improve the growth of carp.Compared with model group, geniposide at middleandhighconcentrations significantly reduced the activities of GPT, GOT and AKP in common carp serum, and alleviated the degree of liver injury in common carp.Lowor middle concentrations of geniposide significantly reduced the levels of TG and LDL-C in serum and the gene expressions of fatp1 and ppar-αin liver tissue, and increased the content of HDL-C in serum, which improved common carp liver lipid metabolism disorder.At the same time, after three concentrations of geniposide treatment, the activity of SOD and the gene expressions of nrf2 and ho-1 in liver tissues were significantly increased, and the expression levels of keap1 gene was significantly decreased, indicating that geniposide could relieve liver oxidative stress caused by BPA.In addition, compared with model group, feeding three concentrations of geniposide significantly down-regulated the gene expressions of myd88, il-1βand il-6, significantly up-regulated the gene expression of il-10, which inhibited the inflammatory response in liver of common carp.【Conclusion】In summary, geniposide can alleviate common carp hepatotoxicity caused by BPA to varying degrees, and reduce hepatotoxicity by improving liver lipid metabolism, enhancing antioxidant capacity, and inhibiting inflammation.Taking into account the actual production cost, it is recommended to use geniposide at middle concentration(2.50 g/kg) as the basis for screening hepatoprotective drugs.