Defence response of local rice variety Acuce in Hani terraced fields in Yunnan against the feeding of Sogatella furcifera

【Objective】To study the main defensive response of Acuce, a local rice variety in the Hani terraced fields in Yuanyang, Yunnan under the feeding stress of white-backed planthopper(WBPH), and to clarify its WBPH resistance mechanism.To provide a basis for rice insect-resistant breeding and utilization of insect-resistant germplasm resources.【Method】The contents of nutrient soluble sugar, secondary products malondialdehyde(MDA) and hydrogen peroxide(H₂O₂) in rice plants were determined by colorimetric method at 0, 3, 6, 12, 24, 48, 72 and 96 h after WBPH feeding on Acuce, insect susceptible variety Taichung Native 1(TN1) and insect resistant variety Rathu Heenati(RHT) in the meantime, the main defense reaction of main protective enzyme activity including peroxidase(POD), phenylalanine ammonia-lyase(PAL) and catalase(CAT) were measured and analyzed.【Result】After WBPH feeding stress 3-96 h, the soluble sugar content of the Acuce increased by 93.19%.The increase rate was significantly lower than TN1(176.23%) (P< 0.05, the same as below), there was no significant difference with RHT(89.16%) (P> 0.05, the same as below).The increase rate of H₂O₂ content was 44.13%, significantly higher than TN1(13.18%) and significantly lower than RHT(83.01%).The content of MDA in the rice plant of Acuce increased, and the mean increase rate was 145.38%, significantly higher than that of TN1(36.84%) and RHT(57.06%).The activities of POD, CAT and PAL were increased after feeding stress.The mean increase rate of POD activity(52.78%) was not significantly different with TN1(52.30%), but was significantly lower than that of RHT(154.53%).The mean increase rate of CAT activity(26.24%) was significantly higher than that of RHT(-9.32%) and TN1(-14.18%).The mean increase rate of PAL activity(3.80%) was significantly higher than that of RHT(-32.01%) and significantly lower than that of TN1(74.03%).【Conclusion】After WBPH feeding, the local rice variety Acuce is more sensitive in nutrients, secondary substances, and major protective enzymes than the insect susceptible variety TN1 and resistant variety RHT, showing a strong defense response.Acuce can be used as the WBPH resistance varieties in the field.