Optimization of Lipu taro tissue culture rapid propagation in temporary immersion bioreactors system

ObjectiveThe rapid propagation system of Lipu taro was optimized by using temporary immersion biore-actors system(TIBs)in order to provide technical supports for industrialized and automated production of its virus-free plantlets.MethodThe adventitious buds induced by meristem of Lipu taro variety Guiyu 2 was used as explants,the suitable hormone combination for multiplication and rooting of adventitious buds was screened by using TIBs,and the effects of different subculture materials,inoculated densities and intermittent frequencies of TIBs on the multiplication and rooting were analyzed.ResultIn TIBs,the multiplication effect of Lipu taro plantlets was enhanced effectively,and the multiplication rate of buds reached 28.96 times,which was as 10 times as that of traditional solid culture(2.87 times);and both of plantlet height and rooting number of induced plantlets were extremely higher than those of traditional solid culture(P<0.01). The hormone combination of 4.00 mg/L 6-BA+0.05 mg/L NAA added in culture medium was the best for multiplication and growth of Lipu taro plantlets in TIBs,and the multiplication rate of plantlet reached 32.04 times. When the 4th sub-cultured plantlet was used as explants,10 plantlets per liter of inoculation densities and 5 min per 6 hours of immersion frequency were found to be the best for growth and multiplication of plantlets,the multiplication rate reached more than 30.00 times,and could shorten the germination of plantlets with well growth vigor in TIBs,and the contamination rate of culture medium was 0.ConclusionThe results showed that it is of highly practicable to use TIBs for rapid propagation of Lipu taro sub-culture plantlets,this will provide important technical supports for implementing and promoting large-scale industrialized production of Lipu taro healthy seedling propagation.