Genetic diversity of Baise Auricularia auricular wild strains in Guangxi

ObjectiveThe genetic diversity of Baise Auricularia auricular wild strains in Guangxi was analyzed,in order to provide reference for protection,exploitation and utilization of the resources of Baise A. auricular.MethodTwenty-three A. auricular wild strains were collected from Baise in Guangxi and artificially cultivated. During this period, their agronomic characters were observed and determined. The polymorphism of esterase isozyme bands in different A. au-ricular strains was analyzed by using esterase isozyme technology,and cluster analysis was also conducted.ResultAmong the 23 wild strains,there were 16 strains with the growth type of individual fruit body,7 strains with clustered fruit body;13 stains had smooth margin and 10 strains had wavy margin;at the back of A. auricular ,15 strains had ob-vious or relatively obvious veins,and 8 strains had no veins or no obvious veins;the surface color of 4 strains was fawn,8 strains tawny,and 11 strains was black brown;the size of surface(length×width)were 30.1-51.6 mm×20.5-41.7 mm and the thickness was 0.95-1.44 mm. These results showed that the morphology characters of Baise A. auricular possessed high diversity. The germination time of primodium varied from 26 to 59 d,and contamination rate of other strains varied from 0 to 51.1%;dry-wet ration of surface varied from 10.1 to 14.1 and yield of first tide varied from 8.2% to 50.5%. These results showed that there were high diversity among different strains on germination time of primordium,anti-weed-moulds,dry-wet ratio and yields. There were 19 bands in total and 18 types of bands detected from the 23 wild strains. The mobility rate(Rf)of bands ranged from 16.6 to 88.6,and the distribution frequency ranged from 4.3% to 100.0%. Three of the bands were the characteristic bands in esterase isozyme bands of Baise A. auricular. The genetic similarity co-efficients ranged from 0.68 to 1.00 among the 23 wild strains. The 23 wild strains could be divided into two groups at the similarity coefficient of 0.68. And the cluster analysis results of most strains were agreed with their geographical distribu-tion.ConclusionBaise A. auricular wild strains in Guangxi possesses high genetic diversity,implies many good genetic characters and has great potential for exploitation and utilization.