Identification and evaluation methods comparison on resistance of rice varieties(combinations)to brown planthopper (Nilaparvata lugens(St?l))

ObjectiveThe resistance of rice varieties(combinations)to brown planthopper(BPH)was identified,the BPH-resistant varieties were screened and methods of resistance identification were also evaluated to provide reference for the future promotion of BPH-resistant varieties.MethodThe rice varieties(combinations)from Institute of Tropical Ag-riculture and Forestry,Hainan University,Key Laboratory of Crop Genetic Improvement,Guangxi Academy of Agricul-tural Sciences and tested Guangxi rice varieties(combinations)in 2017 were used as tested material. BPH were collected from the field directly as well as those fed by the susceptible variety TN1 indoors. Standard seedbox screening technique, honeydew amount mensuration,the survival rates of BPH,feeding selectivity and developmental durations mensuration, oviposition preference of adult BPH and contents of secondary substances mensuration were comprehensively used to identify the resistance of rice varieties(combinations)to BPH.ResultA variety showed resistance(R373)and two va-rieties which showed medium resistance(Teyou 373,Teyou 582)were screened from the tested rice varieties by standard seedbox screening technique. The results of honeydew amount measurement,oviposition preference of adult BPH and contents of secondary substances mensuration were the same as the results of standard seedbox screening technique. All of them showed that there was significant difference between the related indexes of resistance material and the susceptible va-riety TN1(P<0.05),while the difference between related indexes of resistance material and RHT was not significant(P>0.05). However,the results of feeding preference,the survival rates and developmental duration of BPH on rice varieties were not in accordance with the resistance of rice variety,and there is no regulation between the results.ConclusionR373,a stable resistant variety,can be used as basic material in cultivating rice varieties that are resistant to BPH. Stan-dard seedbox screening technique,honeydew amount mensuration,oviposition preference of adult BPH and contents of secondary substances mensuration can be used as identification methods of rice resistance to BPH at seedling stage.