Morphological characteristics of Alosa sapidissima and tissue specificity of two kinds of isozymes in it

ObjectiveThis paper was to enrich the studies about Alosa sapidissima gerplasm resources from the per-spectives of morphological characteristics and biochemical genetics characteristics,and lay the foundation for screening the biochemical genetic markers that could identify A.sapidissima gerplasm resources.MethodCountable traits and mea-surable traits of A.sapidissima were determined by morphometry.By using vertical polyacrylamide gel electrophoresis, lactate dehydrogenase(LDH)and malate dehydrogenase(MDH)from six tissues(heart,eye,muscle,liver,kidney and gill)of A.sapidissima were detected and analyzed.ResultA.sapidissima had a flat fusiform body.Its back and abdomen were shallow arc.And the back were grey and dark,with slight blue-green metallic luster.Both sides of the body and the abdomen were silver white.The fin formulas of A.sapidissima included dorsal fin D.iii-13-15,pectoral fin P.i-14-17,anal fin A.i-ii-16-20,ventral fin V.i-7-9 and caudal fin C.21-25.The morphology of the gill racker was in 7-shape and the num-ber of outer gill racker on the left first gill arch was 24-37+47-64.The isoenzyme band numbers and activity of LDH and MDH from heart,eye,muscle,liver,kidney and gill of A.sapidissima presented tissue specificity and individual varia-tions.ConclusionThe tissue specificity of A.sapidissima isozymes is regulated and controlled by genetic genes,mean-while it is related to the biochemical metabolism activities of the tissues.It is suggested to choose LDH from gill as bio-chemical genetic markers in A.sapidissima gerplasm identification.