Identification of bovine papillomavirus and genome sequence analysis for cattle in Guangxi

ObjetiveWhether cattle in Guangxi have been infected by bovine papillomavirus(BPV)was investigated to provide reference for formulating prevention and control measures of BPV in the future.MethodTumor-shaped sam-ples were collected from the skin of young cattle in a cattle farm in Longan,Guangxi.Based on clinical observation and histopathological observation,BPV were identified and their genotypes were classified by PCR.Then five pairs of speci-fic primers were designed to amplify and sequence BPV genome according to the previous results.ResultHistopathologi-cal slices of the tumor-shaped tissues revealed that,it had epidermis and dermis hyperplasia,and epidermal cells keratini-zation and cavitation occurred.The cells were fusiform and stellate.One strain was isolated from the tumor-shaped sam-ple. Its homology with BPV-1 was 99.0%,and named BPV-GX-LA150909.The full-length genome of strain BPV-GX-LA150909 was 7946 bp,and its GenBank accession number was MF435918.It included three regions:early gene region (E),late gene region(L)and non-coding region(NCR).The homology between strain BPV-GX-LA150909 and BPV-1 type in both genome and L1 gene was the highest,especially with Guizhou strain BPV-1 GZLZ(genome homology 99.9%,L1 gene homology 99.7%). In addition,phylogenetic tree showed that genetic distance of this strain was also close to BPV-1 GZLZ.L1 protein of BPV-GX-LA150909 presented potential B cell epitope,and contained seventeen O-glycosylation sites and one N-glycosylation site.ConclusionBPV-1 infection has been found in Guangxi cattle,and the homology with BPV-1 type at home and abroad is high,which indicates that cross-regional transmission of BPV is a prob-lem that cannot be ignored.