Effects of different anti-browning agent combinations on tissue culture and anti-browning of Dracaena cambodiana Pierre ex Gagnep

ObjectiveEffects of different anti-browning agents and agent combinations on tissue culture and anti-browning of Dracaena cambodiana Pierre ex Gagnep. were studied to provide reference basis for rapid propagation of seedlings.MethodYoung stems of D. cambodiana explants were treated by three kinds of anti-browning agents to test the callus induction and anti-browning. Different combinations of plant growth regulator 6-BA and NAA at various concentrations were added into basic medium MS to detect their effects on adventitious bud differentiation from callus and cluster bud formation of D. cambodiana.ResultThe result showed that the anti-browning rate(20.5%)was lower than CK,and callus induction rate reached the maxi-mum(75.5%)under the treatment of soaking the young stems in 0.1 g/L cysteine(Cys)solution for 10 min and then inoculating it on medium with 0.1 g/L of vitamin C(Vc). Plant growth regulator combination 6.0 mg/L 6-BA+0.5 mg/L NAA was suitable for callus induction of D. cambodiana young stems, and the induction rate was 75.5%. 4.0 mg/L 6-BA+0.2 mg/L NAA combina-tion was optimal for bud proliferation, and the proliferation multiple was 3.3.ConclusionUsing anti-browning agent Cys and Vc can reduce the browning level of young stems in D. cambodiana tissue culture and improve callus induction rate. Adding appropriate amount of 6-BA and NAA combinations into culture medium can promote adventitious bud differentiation from callus and cluster bud formation.