Effects of combination sodium nitraphenolate+GA3 on Cotinus coggygria tissue culture plants

Objective Effects of plant growth regulator combination sodium nitraphenolate+ gibberellin (GA3) at different concentrations on the growth of Cotinus coggygria were explored in order to provide technical support for shortening the culture cycle of tissue culture plants.MethodB5 medic adding sodium nitraphenolate+GA3 of different combinations were used to culture C.coggygria.Leaf number per plant,plant height,fresh weight,dry weight,rooting rate and root number were investigated after 25 d of culture to study the effects of adding sodium nitraphenolate+GA3 on the tissue culture plants.Result 0.020 mg/L sodium nitraphenolate+30.0 mg/L GA3 had the optimal effects on C.coggygria tissue culture plants.Leaf number per plant,plant height,fresh weight,dry weight were significantly higher than those in control (CK) and other treatments (P<0.05).Leaf number per plant,plant height,fresh weight,dry weight increased 83.3%、71.8%、86.6% and 85.8% respectively compared with CK.Rooting was also better than CK and the highest rooting rate reached 95.2%.The plants were strong with large root number,thick leaves and sound growth state.Correlation analysis indicated that there was extremely positive correlation between nitraphenolate+GA3 concentrations and leaf number per plant,plant height,fresh weight,dry weight,rooting rate and root number(P<0.01).ConclusionAdding 0.020 mg/L sodium nitraphenolate+30.0 mg/L GA3 into B5 medium can improve the growth for C.coggygria tissue culture plants,and shorten the culture cycle and speed up the breeding process.