Measurement of genomic C value of five Salvia species by flow cytometry

ObjectiveThe genomic C value of five Salvia species were determined in order to provide reference for further study on genomics, cytobiology and germplasm resource of Salvia species. MethodFresh leaves of S. forskaohlei, S. guaranitica, S. uliginosa, S. hierosolymitana and S. viscosa were taken as samples. The known genome of Oryza sativa subsp. japonica'Nipponbare' was set as internal standard. Genomic C value detection by flow cytometry(FCM) was es-tablished for Salvia. ResultScreened by dissociation liquid, LB01 dissociation liquid was the optimal one for Salvia species. The detected variable coefficient (CV) of five Salvia species and control were smaller than 5.0%. Cell suspension could be tested on equipment after adding propidium iodide(PI) for 5 min staining. The genomic C value of S. forskaohlei, S. guaranitica, S. uliginosa, S. hierosolymitana and S. viscosa were 0.555, 1.170, 1.515, 1.031 and 0.884 pg respectively. And there was significant defference between the C values(P<0.05). ConclusionThe detected C values of five Salvia species can enrich C value database of Salvia. The C value detection method established based on FCM for Salvia species can provide reference for the related research on other Salvia species.