Expression and analysis of differential proteins in Momordica charantia L. seedlings under low temperature stress

ObjectiveIn the present study, the proteins related to cold resistance regulation of Momordica charantia L. at seedling stage were searched for so as to explore mechanism of resistance to low temperature and laid foundation for M. charantia L. cold resistance molecule research and cold resistance variety breeding. MethodCold-susceptible M. charantia L. MC6 and cold-resistant M. charantia L. MC108 were taken as materials. Difference of proteins in M. charantia L. leaves at seedling stage under low temperature stress(8℃for 72 h) was analyzed by two dimensional electrophoresis. Result2-DE map indicated that there were 82 differential protein spots between cold-susceptible M. charantia L. MC6 and cold-re-sistant M. charantia L. MC108. Among these protein spots, thirty-nine up-regulated, thirty-six down-regulated, two were newly added and five disappeared. MALDI-TOF-TOF/MS analysis was conducted on 24 proteins spots with obvious expre-ssion difference. Through searching and matching in database, five unknown proteins and fourteen functional proteins were detected. These proteins were involved in cytophylaxis, radicals scavenging, photosynthesis, basic metabolism and protein degradation. ConclusionUnder stress, M. charantia L. can adapt to the temperature variation through regulating multiple proteins.