Determination of chlorogenic acid content in Pyrrosia produced from different areas of Guangxi

ObjectiveUsing HPLC method, the content of chlorogenic acid in Pyrrosia produced from different areas of Guangxi was determined to provide references for appropriate utilization and development of Pyrrosia medicinal materials in Guangxi. MethodThe content of chlorogenic acid in Pyrrosia was determined by HPLC. Chromatographic conditions were as follows. Chromatographic column was C18. Mobile phase was acetonitrile-0.05% phosphoric acid (11.5∶88.5). Detection wavelength was 326 nm. Flow rate was 1.0 mL/min. Column temperature was 30℃. ResultThere was good linear relation-ship of chlorogenic acid in the range of 0.0368-0.8096μg. The regression equation was y=2607.6x+57.476 (R2=0.9999). Av-erage recovery rate was 100 . 08% ( RSD=0 . 72%) . Vast differences of chlorogenic acid were observed in Pyrrosia from different areas of Guangxi. The highest content of chlorogenic acid was found in Pyrrosia from Luoxiang Village of Jinxiu County (3.6967 mg/g) followed by Pyrrosia from Baoding Waterfall and Dragon Cave scenic region of Ziyuan County with the chlorogenic acid content of 3.4678 and 3.1676 mg/g, respectively. The lowest content of chlorogenic acid was shown in Pyrrosia porosa from Jingxi County (1.1326 mg/g).ConclusionThe HPLC method of measuring chlorogenic acid was simple, accurate and reproductive, thus it’s an important detection method for identifying and evaluating Pyrrosia medicinal materials. The con-tent of chlorogenic acid varied in Pyrrosia from different areas of Guangxi, which may be caused by origin, cultivar or harvest time.