Abstract: Sugarcane streak mosaic virus(SCSMV)displayed symptoms of continuous chlomtic streak on sudan grass (Sorghum sudanense Stapf.)by mechanical sap inoculation.These infected plants(sudan grass) were identified positive for SCSMV with RT-PCR assays with SCSMV specific primer SCSMV-ST2(F) and SCSMV-P1(R).The amplified PCR product from the host plant (sudan grass)was cloned and sequenced and the 0.5kbp product was registered in GenBank with Accession No.EU650178.The BLAST search analysis identified it as SCSMV and confirmed by phylogenetic analysis (MEGA 4.0).This is a new report of sudan grass as an altemative mechanical host for SCSMV.