吴存兵, 陈晓兰, 吴君艳. 2016: 响应面法优化乙醇提取牡丹花总黄酮工艺. 南方农业学报, 47(8): 1370-1375. DOI: 10.3969/j:issn.2095-1191.2016.08.1370
引用本文: 吴存兵, 陈晓兰, 吴君艳. 2016: 响应面法优化乙醇提取牡丹花总黄酮工艺. 南方农业学报, 47(8): 1370-1375. DOI: 10.3969/j:issn.2095-1191.2016.08.1370
WU Cun-bing, CHEN Xiao-lan, WU Jun-yan. 2016: Optimization for extraction process of total flavonoids from peony flower with ethanol as extractor by response surface methodology. Journal of Southern Agriculture, 47(8): 1370-1375. DOI: 10.3969/j:issn.2095-1191.2016.08.1370
Citation: WU Cun-bing, CHEN Xiao-lan, WU Jun-yan. 2016: Optimization for extraction process of total flavonoids from peony flower with ethanol as extractor by response surface methodology. Journal of Southern Agriculture, 47(8): 1370-1375. DOI: 10.3969/j:issn.2095-1191.2016.08.1370

响应面法优化乙醇提取牡丹花总黄酮工艺

Optimization for extraction process of total flavonoids from peony flower with ethanol as extractor by response surface methodology

  • 摘要: 目的采用响应面分析法对牡丹花总黄酮提取工艺进行优化,为其开发利用提供技术参考.方法以牡丹花为原料,在单因素试验基础上,选择乙醇体积分数(A)、液料比(B)、提取时间(C)为影响因素,牡丹花总黄酮提取率(Y)为响应值,采用Box-Behnken试验设计方法,研究各自变量及其交互作用对牡丹花总黄酮提取率的影响.结果牡丹花总黄酮提取率对乙醇体积分数、液料比、提取时间的二次多元回归方程为:Y=5.81+0.29A+0.13B+0.30C+0.24AB+0.45AC+0.24BC-0.50A2-1.42B2-0.61C2(R2=0.9846),该模型拟合程度较好.其中,乙醇体积分数、提取时间及其二者的交互作用对牡丹花总黄酮提取率有极显著影响(P<0.01),乙醇体积分数与液料比、液料比与提取时间的交互作用对提取率有显著影响(P<0.05).乙醇提取牡丹花总黄酮的最佳工艺条件为:乙醇体积分数55%、液料比41:1(mL/g)、提取时间45 min,此条件下牡丹花总黄酮提取率为6.08%,与理论预测值(5.96%)的相对误差为2.01%.结论通过响应面建立的牡丹花总黄酮提取工艺模型拟合效果较好,可用于实际预测.优化后的提取工艺具有所需试剂少、提取时间短、易操作等优点.

     

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