张美华, 刘丽, 俎峰, 王敬乔. 2013: 应用近红外(NIRS)技术分析小样品油菜籽粒含油量及硫甙含量. 南方农业学报, 44(1): 28-32. DOI: 10.3969/j:issn.2095-1191.2013.1.28
引用本文: 张美华, 刘丽, 俎峰, 王敬乔. 2013: 应用近红外(NIRS)技术分析小样品油菜籽粒含油量及硫甙含量. 南方农业学报, 44(1): 28-32. DOI: 10.3969/j:issn.2095-1191.2013.1.28
ZHANG Mei-hua, LIU Li, ZU Feng, WANG Jing-qiao. 2013: Analysis of glucosinolates content in small rapeseed samples by using the Near Infrared Reflectance Spectroscopy (NIRS)technology. Journal of Southern Agriculture, 44(1): 28-32. DOI: 10.3969/j:issn.2095-1191.2013.1.28
Citation: ZHANG Mei-hua, LIU Li, ZU Feng, WANG Jing-qiao. 2013: Analysis of glucosinolates content in small rapeseed samples by using the Near Infrared Reflectance Spectroscopy (NIRS)technology. Journal of Southern Agriculture, 44(1): 28-32. DOI: 10.3969/j:issn.2095-1191.2013.1.28

应用近红外(NIRS)技术分析小样品油菜籽粒含油量及硫甙含量

Analysis of glucosinolates content in small rapeseed samples by using the Near Infrared Reflectance Spectroscopy (NIRS)technology

  • 摘要: 目的对193份甘蓝型油菜籽粒进行常量样品(3.0 g)及小量样品(0.3 g)含油量及硫甙含量近红外光谱分析技术(NIRS)分析和回归模型研究,以期在育种过程中建立实用的油菜小样品含油量及硫甙含量测定数学模型.方法利用NIRS对甘蓝型油菜籽粒进行含油量及硫甙含量测定,并进行回归分析.结果在众多回归模型中,对于小量样品籽粒含油量及硫甙含量,二次模型均为最优拟合模型,回归方程式分别为:y=40.190+0.892x-0.007x2(R2=0.970);y=-92.040+0.748x+0.002x2(R2=0.960)(y为常量测量结果,x为小量测量结果).结论获得的二次回归方程式可以很好地将小量样品测定数据转化为常量样品分析结果,为高含油量和低硫甙油菜品种的选育奠定基础.

     

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