Genetic diversity analysis and fingerprint map construction of sweetpotato based on agronomic traits and molecular markers
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摘要: 【目的】基于农艺性状和分子标记对福建甘薯品种进行遗传多样性分析及指纹图谱构建,为福建省甘薯育种亲本选配和遗传背景研究提供理论依据。【方法】以福建省育成及引进的24个甘薯品种为研究对象,对其农艺性状进行调查测定,利用筛选出的28条ISSR引物和27对SRAP引物进行遗传多样性分析,利用SPSS 26.0的组间联接法和平均欧式距离法进行农艺性状聚类分析;利用NTSYS-pc 2.1进行分子标记聚类分析。基于ISSR和SRAP扩增结果进行指纹图谱构建。【结果】农艺性状聚类分析结果显示,在欧氏距离为8时,24个甘薯品种被分为三大类:第Ⅰ类为泉薯76、泉薯12号和龙薯14号;第Ⅱ类包含徐紫薯2号、泉薯10号、莆薯20、福薯24号、莆紫薯18和福宁紫3号;其余15个品种归在第Ⅲ类。28条ISSR引物和27对SRAP引物分别扩增条带362和364条,多态性比率分别为78.73%和83.79%,部分引物多态率高达100.00%,平均等位基因数(Na)分别为1.7676和1.8307;平均有效等位基因数(Ne)分别为1.4071和1.4631,平均Nei’s基因多样性指数(H')分别为0.2435和0.2730,平均Shannon’s信息指数(I)分别为0.3705和0.4124。基于ISSR分子标记,24个甘薯品种的遗传相似系数为0.674~0.851,在遗传相似系数0.728处可分为三大类:第Ⅰ类仅包含福宁紫3号,第Ⅱ类包含福薯24号、徐紫薯2号、莆薯20、莆薯53、莆紫薯18、莆紫薯3号、福薯404和莆薯16,其余15个品种归为第Ⅲ类。基于SRAP分子标记,24个甘薯品种的遗传相似系数为0.591~0.879,在遗传相似系数0.696处可分为三大类,第Ⅰ类仅包含泉薯76,第Ⅱ类包含莆紫薯18和徐紫薯2号,其余21个品种归为第Ⅲ类。引物UBC899可用于构建24份甘薯种质资源的指纹图谱。【结论】ISSR和SRAP分子标记的多态性检测效率较高,均适用于甘薯种质资源的遗传变异分析。24份供试甘薯材料遗传多样性较丰富,农艺性状聚类结果与分子标记聚类结果相似但又有明显差异,ISSR分子标记聚类结果与种质资源系谱更吻合,引物UBC899可用于供试甘薯品种区分。Abstract: 【Objective】The purpose of the study was to analyze the genetic diversity and construct a fingerprint map of sweetpotato varieties in Fujian based on agronomic traits and molecular markers, so as to provide a theoretical basis for the study of parent selection and genetic background of sweetpotato breeding in Fujian. 【Method】Twenty-four sweetpotato varieties bred and introduced in Fujian were used as research subjects to investigate and determine their agronomic traits. The 28 ISSR primers and 27 SRAP primers screened were used for genetic diversity analysis. SPSS 26.0 was used to perform clustering analysis of agronomic traits using the intergroup connection method and the mean Euclidean distance method. NTSYS-pc 2.1 was used for molecular marker clustering analysis. The fingerprint map was constructed according to the ISSR and SRAP amplification results. 【Result】The results of agronomic traits clustering analysis showed that 24sweetpotato varieties were classified into three major groups at a Euclidean distance of 8. GroupⅠconsisted of Quanshu 76, Quanshu No. 12 and Longshu No. 14. GroupⅡcontained Xuzishu No. 2, Quanshu No. 10, Pushu 20, Fushu No. 24, Puzishu 18 and Funingzi No. 3. The remaining 15 species were classified in Group III. The 28 ISSR primers and 27 pairs of SRAP primers amplified 362 and 364 bands respectively, with a polymorphism ratio of 78.73% and 83.79%, and the polymorphism rate of some primers was as high as 100.00%. The average numbers of alleles(Na) were 1.7676 and 1.8307, respectively, the average numbers of effective alleles(Ne) were 1.4071 and 1.4631, the average Nei's gene diversity index(H') were 0.2435 and 0.2730, the average Shannon's information index(I) were 0.3705 and 0.4124, respectively. Based on ISSR molecular markers, the genetic similarity coefficients of the 24 sweetpotato varieties ranged from 0.674 to 0.851, and they could be categorized into three major groups at the genetic similarity coefficient of 0.728.GroupⅠcontained only Funingzi No. 3, groupⅡcontained Fushu No. 24,Xuzishu No. 2,Pushu 20,Pushu 53,Puzishu 18,Puzishu No. 3,Fushu 404 and Pushu 16, and the remaining 15 varieties were categorized into group III. Based on SRAP molecular markers, the genetic similarity coefficient of the 24 sweetpotato varieties ranged from 0.591 to 0.879, and they could be categorized into three major groups at the genetic similarity coefficient of 0.696. Group Ⅰ contained only Quanshu 76, group Ⅱ contained Puzishu 18 and Xuzishu No. 2, and the remaining 21 varieties were categorized into group Ⅲ. The primer UBC899 could be used to construct the fingerprint map of 24 sweetpotato germplasm resources.【Conclusion】ISSR and SRAP molecular markers are efficient in polymorphism detection, and both of them are suitable for genetic variation analysis of sweetpotato germplasm resources. The genetic diversity of the 24 sweetpotato materials for tes-ting is relatively rich, and the clustering results based on agronomic traits cluster results and molecular markers are similar but greatly different. The ISSR molecular clustering results are more consistent with the pedigree map of the germplasm resources, and the primer UBC899 can be used to distinguish the tested sweetpotato varieties.
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Key words:
- sweetpotato /
- molecular marker /
- genetic diversity /
- fingerprint map
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崔纪超, 余金姜, 中奕, 钟玉扬, 郑建扬.2018.甘薯×木薯远缘杂交与甘薯种质创新[J].热带农业科学, 38(12):56-63.[Cui J C, Yu J J, Zhong Y, Zhong Y Y, Zheng J Y.2018.Distant hybridization between sweetpotato and cassava and enhancement of sweet potato germplasm[J].Chinese Journal of Tropical Agriculture, 38(12):56-63.]doi: 10.12008/j.issn.1009-2196.2018.12.012. 邓禄军, 吴巧玉, 何天久, 邓仁菊, 王启富, 罗密.2021.基于形态学和ISSR分子标记的迷你甘薯遗传多样性分析[J].种子, 40(10):56-61.[Deng L J, Wu Q Y, He T J, Deng RJ, Wang Q F, Luo M.2021.Genetic diversity analysis of mini sweet potato based on morphology and ISSR molecu-lar markers[J].Seed, 40(10):56-61.]doi: 10.16590/j.cnki.1001-4705.2021.10.056. 郝玉民, 郭兰, 韩延闯, 刁英, 杨新笋, 胡中立.2007.甘薯品种的SRAP遗传多样性分析[J].武汉植物学研究, 25(4):406-409.[Hao Y M, Guo L, Han Y C, Diao Y, Yang X S, Hu Z L.2007.Genetic diversity analysis of sweet potato based on SRAP markers[J].Journal of Wuhan Botanical Research, 25(4):406-409.]doi: 10.3969/j.issn.2095-0837.2007.04.015. 贺学勤, 刘庆昌, 翟红, 王玉萍.2005.用RAPD、ISSR和AFLP标记分析系谱关系明确的甘薯品种的亲缘关系[J].作物学报, 31(10):1300-1304.[He X Q, Liu Q C, Zhai H, Wang Y P.2005.The use of RAPD, ISSR and AFLP markers for analyzing genetic relationships among sweetpotato cultivars with known origin[J].Acta Agronomica Sinica, 31(10):1300-1304.]doi: 10.3321/j.issn:0496-3490.2005.10.010. 贺学勤.2004.中国甘薯地方品种的遗传多样性分析[D].北京:中国农业大学.[He X Q.2004.Aanlysis of genetic divesrity among sweetpotato landarees in China[D].Beijing:China Agricultural University.]doi: 10.7666/d.y658941. 季志仙, 王美兴, 范宏环, 吴列洪, 朱金庆.2014.基于ISSR指纹的甘薯食用品种的遗传多样性分析[J].核农学报, 28(7):1197-1202.[Ji Z X, Wang M X, Fan H H, Wu L H, Zhu J Q.2014.Genetic diversity assessment of edible sweetpotato germplasm based on ISSR fingerprint[J].Journal of Nuclear Agricultural Science, 28(7):1197-1202.]doi: 10.11869/j.issn.100-8551.2014.07.1197. 李慧峰, 黄咏梅, 李彦青, 滑金锋, 吴翠荣, 范继征, 陈天渊.2019.基于SLAF-seq技术的甘薯种质资源群体遗传进化分析[J].热带作物学报, 40(12):2390-2396.[Li H F, Huang Y M, Li Y Q, Hua J F, Wu C R, Fan J Z, Chen T Y.2019.Phylogenetic analysis of sweetpotato germplasm resources based on SLAF-seq technology[J].Chinese Journal of Tropical Crops, 40(12):2390-2396.]doi: 10.3969/j.issn.1000-2561.2019.12.011. 刘中华, 林志坚, 李华伟, 许泳清, 李国良, 邱永祥, 邱思鑫, 汤浩.2019.甘薯种质资源遗传多样性的ISSR分析[J].南方农业学报, 50(11):2392-2400.[Liu Z H, Lin Z J, Li HW, Xu Y Q, Li G L, Qiu Y X, Qiu S X, Tang H.2019.Genetic diversity analysis of sweetpotato[Ipomoea batatas(L.)Lam.]by ISSR molecular markers[J].Journal of Southern Agriculture, 50(11):2392-2400.]doi: 10.3969/j.issn.2095-1191.2019.11.03. 罗小敏.2009.国内主要甘薯种质资源的遗传多样性研究[D].重庆:西南大学.[Luo X M.2009.Study on genetic diversity among main sweetpotato germplasm resources in China[D].Chongqing:Southwest University.] 穆国俊, 何丽君, 李朋飞, 杜丽芬, 段会军, 崔顺立, 孟庆荣, 刘立峰.2013.利用农艺性状和SRAP标记方法初步鉴定甘薯丰收白突变体[J].植物遗传资源学报, 14(4):728-733.[Mu G J, He L J, Li P F, Du L F, Duan H J, Cui S L, Meng Q R, Liu L F.2013.Preliminary identification to one mutant of sweetpotato variety Fengshoubai by agronomic trait and SRAP marker analysis[J].Journal of Plant Genetic Resources, 14(4):728-732.]doi: 10.13430/j.cnki.jpgr.2013.04.018. 邱永祥, 许泳清, 刘中华, 罗文彬, 李华伟, 汤浩, 蔡南通.2021.“十一五”期间福建省甘薯育种研究进展及展望[J].福建农业学报, 26(4):676-680.[Qiu Y X, Xu Y Q, Liu Z H, Luo W B, Li H W, Tang H, Cai N T.2021.Advances and prospect in sweet potato breeding for Fujian, 2006-2010[J].Fujian Journal of Agricultural Sciences, 26(4):676-680.]doi: 10.19303/j.issn.1008-0384.2011.04.036. 石广丽, 秦红艳, 刘海双, 李昌禹, 赵滢, 杨义明, 范书田, 艾军.2018.基于SSR分子标记及田间农艺性状的软枣猕猴桃资源鉴别[J].分子植物育种, 16(23):7747-7753.[Shi G L, Qin H Y, Liu H S, Li C Y, Zhao Y, Yang Y M, Fan S T, Ai J.2018.Identification of Actinidia argute resources based on SSR markers and the field agronomic traits[J].Molecular Plant Breeding, 16(23):7747-7753.]doi: 10.13271/j.mpb.016.007747. 宋吉轩, 雷尊国, 丁海兵, 李云.2011.贵州甘薯地方种质资源ISSR遗传多样性分析[J].种子, 30(3):76-80.[Song J X, Lei Z G, Ding H B, Li Y.2011.Analysis on genetic diver-sity of local sweetpotato germplasm resources in Guizhou by ISSR[J].Seed, 30(3):76-80.]doi: 10.16590/j.cnki.1001-4705.2011.03.053. 宋吉轩.2010.贵州甘薯种质资源鉴定与ISSR遗传多样性分析[D].重庆:西南大学.[Song J X.2010.Guizhou sweet-potato germplasm resources determination and ISSR analy-sis of genetic diversity[D].Chongqing:Southwest Univer-sity.]doi: 10.16590/j.cnki.1001-4705.2017.12.017. 王崇.2020.甘薯cpSSR和TRAP分子标记的开发及遗传多样性分析[D].荆州:长江大学.[Wang C.2020.Develop-ment and genetic diversity analysis of cpSSR and TRAPmolecular markers in sweet potato[D].Jingzhou:Yangtze University.]doi: 10.26981/d.cnki.gjhsc.2020.000521 王红意, 翟红, 王玉萍, 何绍贞, 刘庆昌.2009.30个中国甘薯主栽品种的RAPD指纹图谱构建及遗传变异分析[J].分子植物育种, 7(5):879-884.[Wang H Y, Zhai H, Wang YP, He S Z, Liu Q C.2009.RAPD fingerprints and genetic variations of the 30 main sweetpotato varieties in China[J].Molecular Plant Breeding, 7(5):879-884.]doi: 10.3969/mpb.007.000879. 王廷章, 王芳平.1984.甘薯与木薯远缘杂交后代的同工酶研究[J].福建师大学报(自然科学版), (2):97-104.[Wang T Z, Wang F P.1984.Studies on iso-enzymes in the progeny of distant hybrids between sweet potato and cassava[J].Journal of Fujian Normal University(Natural Science Edition), (2):97-104.] 吴洁, 谭文芳, 何俊蓉, 蒲志刚, 王大一, 张正圣, 詹付凤, 阎文昭.2005.甘薯SRAP连锁图构建淀粉含量QTL检测[J].分子植物育种, 3(6):841-845.[Wu J, Tan W F, He J R, Pu Z G, Wang D Y, Zhang Z S, Zhan F F, Yan W Z.2005.Construct on of SRAP linkage map and QTL mapping for starch content in sweetpotato[J].Molecular Plant Breeding, 3(6):841-845.]doi: 10.3969/j.issn.1672-416X.2005.06.015. 吴洁, 谭文芳, 阎文昭, 钟昌松, 王大一.2007.甘薯种质资源亲缘关系SRAP标记分析[J].四川大学学报(自然科学版), 44(4):878-882.[Wu J, Tan W F, Yan W Z, Zhong CS, Wang D Y.2007.Genetic relationship of sweet potato cultivars analysised by sequence-related amplified poly-morphism[J].Journal of Sichuan University(Natural Science Edition), 44(4):878-882.]doi: 10.3969/j.issn.0490-6756.2007.04.032. 武小霞, 崔纪超, 钟玉扬, 余金姜, 颜墩炜, 朱锦乐, 郑建扬, 中奕.2021.福建省甘薯育成品种的遗传多样性分析[J].热带农业科学, 41(7):57-62.[Wu X X, Cui J C, Zhong YY, Yu J J, Yan D W, Zhu J L, Zheng J Y, Zhong Y.2021.Genetic diversity analysis of sweetpotato varieties bred in Fujian[J].Chinese Journal of Tropical Agriculture, 41(7):57-62.]doi: 10.12008/j.issn.1009-2196.2021.07.010. 姚祝芳, 张雄坚, 杨义伶, 黄立飞, 陈新亮, 姚肖健, 罗忠霞, 陈景益, 王章英, 房伯平.2022.177份甘薯地方资源表型性状的遗传多样性分析[J].作物学报, 48(9):2228-2241.[Yao Z F, Zhang X J, Yang Y L, Huang L F, Chen X L, Yao X J, Luo Z X, Chen J Y, Wang Z Y, Fang B P.2022.Genetic diversity of phenotypic traits in 177 sweetpotato landraces[J].Acta Agronomica Sinica, 48(9):2228-2241.]doi: 10.3724/SP.J.1006.2022.14166. 易燚波.2008.利用ISSR标记对甘薯种质资源的遗传多样性分析[D].重庆:西南大学.[Yi Y B.2008.ISSR analysis of genetic diversity in sweetpotato (Ipomoea batatas) germ-plasm resource[D].Chongqing:Southwest University.] 余慧霞, 刘彩霞, 李清峰, 许丽娜, 王彦青, 姚明明, 陈佳静, 孙刚, 王掌军.2022.普通小麦及其亲缘种农艺与籽粒品质性状及金属元素含量相关分子标记分析[J].南方农业学报, 53(7):1821-1831.[Yu H X, Liu C X, Li Q F, Xu L N, Wang Y Q, Yao M M, Chen J J, Sun G, Wang Z J.2022.Analysis of agronomic and grain quality traits and molecu-lar markers related to metal element content in common wheat and its related species[J].Journal of Southern Agri-culture, 53(7):1821-1831.]doi: 10.3969/j.issn.2095-1191.2022.07.004. 张安世, 许会才, 王育水.2015a.22个甘薯品种的ISSR指纹图谱构建及遗传多样性分析[J].河南农业科学, 44(11):11-15.[Zhang A S, Xu H C, Wang Y S.2015a.Construc-tion of fingerprinting and analysis of genetic diversity with ISSR markers for 22 sweetpotato varieties[J].Journal of Henan Agricultural Sciences, 44(11):11-15.]doi: 10.15933/j.cnki.1004-3268.2015.11.003. 张安世, 张利民, 徐九文.2015b.甘薯SRAP指纹图谱构建及遗传多样性分析[J].生物技术, 25(2):161-164.[Zhang A S, Zhang L M, Xu J W.2015b.Construction of finger-printing and analysis of genetic diversity with SRAP markers for sweetpotato[J].Biotechnology, 25(2):161-164.]doi: 10.16519/j.cnki.1004-311x.2015.02.033. 张凯, 罗小敏, 蒋玉春, 吴正丹, 王季春, 唐道彬.2013.甘薯种质资源的SRAP鉴定及遗传多样性分析[J].核农学报, 27(5):568-575.[Zhang K, Luo X M, Jiang Y C, Wu Z D, Wang J C, Tang D B.2013.Genetic diversity among main sweetpotato germplasm resources using SRAP markers[J].Journal of Nuclear Agricultural Sciences, 27(5):568-575.]doi: 10.11869/hnxb.2013.05.0568. 张奕.2007.甘薯品种亲缘关系的RAPD和ISSR分子标记分析[D].福州:福建农林大学.[Zhang Y.2007.RAPD and ISSR analysis in genetic relationship among varieties of sweetpotato[D].Fuzhou:Fujian Agriculture and Forestry University.] 张允刚, 房伯平.2006.甘薯种质资源描述规范和数据标准[M].北京:中国农业出版社.[Zhang Y G, Fang B P.2006.Description specification and data standard of sweet-potato germplasm resources[M].Beijing:China Agricul-ture Press.] Katayama K, Kobayashi A, Sakai T, Kuranouchi T, Kai Y.2017.Recent progress in sweetpotato breeding and culti-vars for diverse applications in Japan[J].Breeding Science, 67(1):3-14.doi: 10.1270/jsbbs.16129.
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