宁春4号×河东乌麦F<sub>6:7</sub>家系籽粒蛋白质性状及候选基因分析

陈佳静; 毛馨缘; 王新杰; 李清峰; 刘彩霞; 张雪婷; 赵补全; 亢玲; 李前荣; 王掌军

doi:10.3969/j.issn.2095-1191.2023.12.003

宁春4号×河东乌麦F_6:7家系籽粒蛋白质性状及候选基因分析

doi: 10.3969/j.issn.2095-1191.2023.12.003

1 宁夏大学农学院, 宁夏银川 750021;
2 宁夏农林科学院农作物研究所, 宁夏银川 750001

基金项目:

宁夏自然科学基金项目(2023AAC03060)

宁夏农业育种专项(2023NYYZ02)

详细信息

作者简介:
陈佳静(1997-),https://orcid.org/0009-0007-9568-815X,研究方向为作物育种技术,E-mail:chenjiajing1010@163.com

通讯作者:
王掌军(1978-),https://orcid.org/0000-0002-1585-8347,博士,副教授,主要从事小麦遗传育种研究工作,E-mail:wangzj-gs@126.com

中图分类号: S512.103.53
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出版历程
- 收稿日期: 2023-07-01

Grain protein traits and candidate genes analysis in F_6:7 pedigrees derived from Ningchun No. 4×Hedong black wheat

1 Agricultural College, Ningxia University, Yinchuan, Ningxia 750021, China;
2 Institute of Crop Sciences, Ningxia Academy of Agricultural-forestry Sciences, Yinchuan, Ningxia 750001, China

Funds:

Ningxia Natural Science Foundation (2023AAC03060)

Ningxia Agriculture Breeding Special Projec(t2023NYYZ02)

摘要

摘要: 【目的】对宁春4号和河东乌麦杂交、自交F_6:7家系进行籽粒蛋白质性状及候选基因分析,以期挖掘与籽粒蛋白质性状相关的基因,为小麦籽粒蛋白质性状遗传改良提供理论参考。【方法】以蛋白质性状差异较大的2个亲本(宁春4号和河东乌麦)及其杂交、自交的551个F_6:7家系为材料,对小麦籽粒蛋白质性状(粗蛋白含量、湿面筋含量和沉降值)进行方差分析、相关分析及聚类分析,通过高、低值株系转录组测序,对差异表达基因(DEGs)进行功能注释及信号通路富集分析,并鉴定籽粒蛋白质性状相关基因。【结果】籽粒蛋白质相关性状在F_6:7家系中均出现明显分离,分布均呈连续正态分布。粗蛋白含量、湿面筋含量和沉降值的平均值分别为15.64%、33.31%和39.06 mL,介于双亲该性状值之间,其中,超中亲比例为29.04%~77.31%,超高亲比例为7.08%~39.56%;变异系数排序为沉降值(10.99%)>湿面筋含量(6.07%)>粗蛋白含量(5.70%),且三者间呈极显著正相关(P<0.001)。551个F_6:7家系被聚为四大类群(Ⅰ~Ⅳ),分别包括148、125、171和107个家系,其中,类群Ⅳ的平均粗蛋白含量(16.87%)、湿面筋含量(35.96%)和沉降值(45.02 mL)均最高,为优异类群。根据高、低值株系的转录组测序数据,从中筛选出4368个DEGs,其中,970个为上调表达,3398个为下调表达。GO功能注释结果显示,小麦籽粒蛋白质性状差异表达主要发生在细胞区域,涉及细胞膜结构和细胞器等组分,同时也发生在细胞连接等部位,通过调节结合、催化等分子功能,引起细胞过程、代谢过程、胁迫反应和应激反应为主的生物过程协同变化。KEGG信号通路富集结果显示,DEGs显著富集到9条代谢通路(P<0.05),其中筛选出4条与小麦籽粒蛋白质性状密切相关的代谢通路及其DEGs,10个DEGs与组氨酸代谢、21个与淀粉和蔗糖代谢、8个与酪氨酸代谢、8个与丙氨酸和天冬氨酸及谷氨酸代谢相关,其中有7个DEGs上调表达。实时荧光定量PCR验证结果与转录组测序结果基本一致。【结论】小麦籽粒蛋白质性状属多基因控制的数量性状遗传,F_6:7家系出现许多超亲类型,其中沉降值具有较大改良潜力。类群Ⅳ为籽粒蛋白质性状优异类群,可用于蛋白质性状优异品种的育种实践。基于富集功能分析筛选出组氨酸代谢、淀粉和蔗糖代谢、酪氨酸代谢、丙氨酸和天冬氨酸及谷氨酸代谢4条通路与小麦籽粒蛋白质性状调控机制有关,并鉴定出7个与籽粒蛋白质性状相关的候选基因。
- 小麦 /
- 家系 /
- 粗蛋白含量 /
- 湿面筋含量 /
- 沉降值 /
- 转录组
Abstract: 【Objective】Analysis of grain protein traits and candidate genes in F_6:7 pedigrees of Ningchun No. 4 and Hedong black wheat through the cross and self-cross was carried out to discover candidate genes associated with grain protein traits, which would provide theoretical reference for genetic improvement of grain protein traits in wheat. 【Method】551 F_6:7 pedigrees deriving from the cross and self-cross between two parents(Ningchun No. 4 and Hedong black wheat) which showed significant differences in grain protein traits, were used as materials. Variance analysis, correlation analysis and cluster analysis were performed to analyze the grain protein traits(crude protein content, wet gluten content and sedimentation value), transcriptome sequencing, functional annotation of differentially expressed genes(DEGs), signaling pathway enrichment analysis were performed for high and low value strains, and genes related to grain protein traits were identified. 【Result】The grain protein-related traits in the F_6:7 pedigrees were obviously separated,and the distribution was continuous normal distribution. The average values of crude protein content,wet gluten content and sedimentation value were 15.64%, 33.31% and 39.06 mL, respectively, between the values of the traits of both parents. The proportions of pedigrees exceeding the mid-parent value ranged from 29.04% to 77.31%,and the proportions of pedigrees exceeding the high-parent value ranged from 7.08% to 39.56%. The order of variation coefficient was sedimentation value(10.99%)>wet gluten content(6.07%)>crude protein content(5.70%),and the three traits showed extremely significant positive correlation(P<0.001). The 551 F_6:7 pedigrees were clustered into four groups (Ⅰ-Ⅳ),including 148,125,171 and 107 materials,respectively. Group Ⅳ displayed the highest average crude protein content(16.87%),wet gluten content(35.96%) and sedimentation value(45.02 mL),making it the superior group. According to the transcriptome sequencing data of high and low value strains, 4368 DEGs were screened out,in which there were 970 up-regulated genes and 3398 downregulated genes. GO functional annotation results showed that the differential expression of wheat protein traits mainly occurred in the cellular part, involving cell membrane structure and organelles, but also occurred in cell junctions parts, through regulating molecular functions such as binding and catalysis, it caused synergistic changes in biological processes, mainly cellular processes, metabolic processes, stress responses and stimulation responses. KEGG signaling pathway enrichment results showed that the DEGs were significantly(P<0.05) enriched into nine metabolism pathways,four metabolism pathways were screened and their enriched DEGs were closely related to wheat grain protein traits,10 DEGs were related to histidine metabolism,21 related to starch and sucrose metabolism,8 related to tyrosine metabolism,8 related to alanine,aspartate and glutamate metabolism. In them,there were 7 up-regulated expression genes. The results of real-time fluorescence quantitative PCR verification were basically consistent with the transcriptome sequencing results.【Conclusion】The grain protein traits belong to quantitative trait inheritance controlled by multiple genes in wheat,and many super-parents types were appeared in F_6:7 pedigrees. Sedimentation value had greater potential for improvement.Group Ⅳ is superior group in grain protein traits and can be used in the breeding practices of varieties with superior protein traits. Four pathways are screened,including histidine metabolism,starch and sucrose metabolism,tyrosine metabolism,and alanine,aspartate and glutamate metabolism based on enrichment functional analysis,are related to the regulation mechanism of wheat grain protein traits. Seven candidate genes are identified possibly related to wheat grain protein traits.
- wheat /
- pedigree /
- crude protein content /
- wet gluten content /
- sedimentation value /
- transcriptome

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参考文献(41)

何中虎, 夏先春, 陈新民, 庄巧生.2011.中国小麦育种进展与展望[J].作物学报, 37(2):202-215.[He Z H, Xia X C, Chen X M, Zhuang Q S.2011.Progress of wheat breeding in China and the future perspective[J].Acta Agronomica Sinica, 37(2):202-215.]doi: 10.3724/SP.J.1006.2011.00202.

何中虎, 晏月明, 庄巧生, 张艳, 夏先春, 张勇, 王德森, 夏兰芹, 胡英考, 蔡民华, 陈新民, 阎俊, 周阳.2006.中国小麦品种品质评价体系建立与分子改良技术研究[J].中国农业科学, 39(6):1091-1101.[He Z H, Yan Y M, Zhuang QS, Zhang Y, Xia X C, Zhang Y, Wang D S, Xia L Q, Hu YK, Cai M H, Chen X M, Yan J, Zhou Y.2006.Establish-ment of quality evaluation system and utilization of molecu-lar methods for the improvement of Chinese wheat quality[J].Scientia Agricultura Sinica, 39(6):1091-1101.]doi: 10.3321/j.issn:0578-1752.2006.06.002.

王掌军, 许娜丽, 王新华, 马冬花, 杨杰, 李清峰, 刘彩霞, 刘凤楼, 亢玲, 张双喜.2021.宁春4号与河东乌麦杂交F2:5家系的籽粒品质性状及其QTL分析[J].南方农业学报, 52(9):2391-2403.[Wang Z J, Xu N L, Wang X H, Ma D H, Yang J, Li Q F, Liu C X, Liu F L, Kang L, Zhang S X.2021.Analysis of grain quality traits and their QTL using F2:5 pedigrees derived from Ningchun No.4×Hedong black wheat[J].Journal of Southern Agriculture, 52(9):2391-2403.]doi: 10.3969/j.issn.2095-1191.2021.09.009.

许娜丽, 王新华, 马冬花, 杨杰, 李清峰, 刘凤楼, 刘彩霞, 刘根红, 张晓岗, 王掌军.2021.251份小麦种质资源的主要农艺与品质性状遗传多样性分析[J].南方农业学报, 52(9):2404-2416.[Xu N L, Wang X H, Ma D H, Yang J, Li Q F, Liu F L, Liu C X, Liu G H, Zhang X G, Wang Z J.2021.Genetic diversity analysis of main agronomic and quality traits of 251 wheat germplasm resources[J].Jour-nal of Southern Agriculture, 52(9):2404-2416.]doi: 10.3969/j.issn.2095-1191.2021.09.010.

严勇亮, 时晓磊, 张金波, 耿洪伟, 肖菁, 路子峰, 倪中福, 丛花.2021.春小麦籽粒主要品质性状的全基因组关联分析[J].中国农业科学, 54(19):4033-4047.[Yan Y L, Shi X L, Zhang J B, Geng H W, Xiao J, Lu Z F, Ni Z F, Cong H.2021.Genome-wide association study of grain quality related characteristics of spring wheat[J].Scientia Agricul-tura Sinica, 54(19):4033-4047.]doi: 10.3864/j.issn.0578-1752.2021.19.001.

张玉峰, 杨武德, 白晶晶, 王大成, 牛波, 冯美臣.2006.冬小麦产量与籽粒蛋白质含量协同变化特点及水肥调控[J].中国农业科学, 39(12):2449-2458.[Zhang Y F, Yang W D, Bai J J, Wang D C, Niu B, Feng M C.2006.Coordinated variation of yield and grain protein content in winter wheat and strategies of irrigation and fertilization[J].Scientia Agricultura Sinica, 39(12):2449-2458.]doi: 10.3321/j.issn:0578-1752.2006.12.008.

Blaise M, Fréchin M, Oliéric V, Charron C, Sauter C, Lorber B, Roy H, Kern D.2011.Crystal structure of the archaeal asparagine synthetase:Interrelation with aspartyl-tRNAand asparaginyl-tRNA synthetases[J].Journal of Molecu-lar Biology, 412(3):437-452.doi: 10.1016/j.jmb.2011.07.050.

Brosnan M E, Brosnan J T.2020.Histidine metabolism and function[J].The Journal of Nutrition, 150(S1):2570S-2575S.doi: 10.1093/JN/NXAA079.

Chouvarine P, Hansmann G.2021.Construction of transcrip-tional regulatory networks using total RNA-seq data[J].STAR Protocols, 2(3):100769.doi: 10.1016/j.xpro.2021.100769.

Conti V, Roncallo P F, Beaufort V, Cervigni G L, Miranda R, Jensen C A, Echenique V C.2011.Mapping of main and epistatic effect QTLs associated to grain protein and gluten strength using a RIL population of durum wheat[J].Jour-nal of Applied Genetics, 52(3):287-298.doi: 10.1007/s13353-011-0045-1.

Duncan O, Trösch J, Fenske R, Taylor N L, Millar A H.2017.Resource:Mapping the Triticum aestivum proteome[J].The Plant Journal, 89(3):601-616.doi: 10.1111/tpj.13402.

Florea L, Song L, Salzberg S L.2013.Thousands of exon skip-ping events differentiate among splicing patterns in sixteen human tissues[J].F1000Research, 2:188.doi: 10.12688/f1000research.2-188.v2.

Furtado A, Bundock P C, Banks P M, Fox G, Yin X, Henry R J.2015.A novel highly differentially expressed gene in wheat endosperm associated with bread quality[J].Scien-tific Reports, 5(1):10446.doi: 10.1038/srep10446.

Geyer M, Mohler V, Hartl L.2022.Genetics of the inverse rela-tionship between grain yield and grain protein content in common wheat[J].Plants(Basel), 11(16):2146.doi: 10.3390/plants11162146.

Goel S, Singh K, Singh B, Grewal S, Dwivedi N, Alqarawi AA, Abdallah E F, Ahmad P, Singh N K.2019.Analysis of genetic control and QTL mapping of essential wheat grain quality traits in a recombinant inbred population[J].PLoSOne, 14(3):e0200669.doi: 10.1371/journal.pone.0200669.

Guo Y, Zhang G Z, Guo B J, Qu C Y, Zhang M X, Kong F M, Zhao Y, Li S S.2020.QTL mapping for quality traits using a high-density genetic map of wheat[J].PLoS One, 15(3):e0230601.doi: 10.1371/journal.pone.0230601

Guzha A, Mcgee R, Scholz P, Hartken D, Lvdke D, Bauer K, Wenig M, Zienkiewicz K, Cornelia H, Feussner L, Vlot AC, Wiermer M, Haughn G, Ischebeck T.2022.Cell walllocalized BETA-XYLOSIDASE4 contributes to immunity of Arabidopsis against Botrytis cinerea[J].Plant Physiology, 189(3):1794-1813.doi: 10.1093/plphys/kiac165.

Han M, Zhang C, Suglo P, Sun S Y, Wang M Y, Su T.2021.L-Aspartate:An essential metabolite for plant growth and stress acclimation[J].Molecules, 26(7):1887.doi: 10.3390/molecules26071887.

Hu X G, Wu B H, Liu D C, Wei Y M, Gao S B, Zheng Y L.2013.Variation and their relationship of NAM-G1 gene and grain protein content in Triticum timopheevii Zhuk[J].Journal of Plant Physiology, 170(3):330-337.doi: 10.1016/j.jplph.2012.10.009.

Jin X F, Feng B, Xu Z B, Fan X L, Liu J, Liu Q, Zhu P, Wang T.2018.TaAAP6-3B, a regulator of grain protein content selected during wheat improvement[J].BMC Plant Biology, 18(1):71.doi: 10.1186/s12870-018-1280-y.

Kim D, Langmead B, Salzberg S L.2015.HISAT:A fast spliced aligner with low memory requirements[J].Nature Methods, 12(4):357-360.doi: 10.1038/nmeth.3317.

Kumar R, Singh R.1981.Free sugars and their relationship with grain size and starch content in developing wheat grains[J].Journal of the Science of Food and Agriculture, 32(3):229-234.doi: 10.1002/jsfa.2740320305.

Lapitan N L V, Hess A, Cooper B, Botha A M, Badillo D, Iyer H, Menert J, Close T, Wright L, Hanning G, Tahir M, Law-rence C.2009.Differentially expressed genes during malting and correlation with malting quality phenotypes in bar-ley (Hordeum vulgare L.)[J].Theoretical and Applied Genetics, 118(5):937-952.doi: 10.1007/s00122-008-0951-8.

Lee M H, Choi C, Kim K H, Son J H, Lee G E, Choi J Y, Kang C S, Shon J, Ko J M, Kim K M.2023.Generation of wheat near-isogenic lines overexpressing 1Bx7 glutenin with increased protein contents and SDS-sedimentation values[J].Plants, 12(6):1244.doi: 10.3390/plants12061244.

Li H M, Tang Z X, Zhang H Q, Yan B J, Ren Z L.2013.Major quality trait analysis and QTL detection in hexaploid wheat in humid rain-fed agriculture[J].Genetics and Molecular Research, 12(2):1740-1751.doi: 10.4238/2013.May.21.5.

Li N, Miao Y P, Ma J F, Zhang P P, Chen T, Liu Y, Che Z, Sha-hinnia F, Yang D L.2023.Consensus genomic regions for grain quality traits in wheat revealed by Meta-QTL analy-sis and in silico transcriptome integration[J].The Plant Genome, 16(2):e20336.doi: 10.1002/tpg2.20336.

Liepman A H, Olsen L J.2004.Genomic analysis of amino-transferases in Arabidopsis thaliana[J].Critical Reviews in Plant Sciences, 23(1):73-89.doi: 10.1080/07352680490273419.

Love M I, Huber W, Anders S.2014.Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2[J].Genome Biology, 15(12):1-21.doi: 10.1186/s13059-014-0550-8.

Lv L, Zhao A, Zhang Y, Li H, Chen X Y.2021.Proteome and transcriptome analyses of wheat near isogenic lines identi-fies key proteins and genes of wheat bread quality[J].Scientific Reports, 11(1):9978.doi: 10.1038/s41598-021-89140-4.

Ma M M, Li Y C, Xue C, Xiong W, Peng Z P, Han X, Ju H, He Y.2021.Current situation and key parameters for improving wheat quality in China[J].Frontiers in Plant Science, 12:638525.doi: 10.3389/fpls.2021.638525.

Mut Z, Aydin N, Bayramoglu H O, Ozcan H.2010.Stability of some quality traits in bread wheat (Triticum aestivum)genotypes[J].Journal of Environmental Biology, 31(4):489.doi: 10.1038/srep31721.

Nedelkou I P, Maurer A, Schubert A, Léon J, Pillen K.2017.Exotic QTL improve grain quality in the tri-parental wheat population SW84[J].PLoS One, 12(7):e0179851.doi: 10.1371/journal.pone.0179851.

Pertea M, Pertea G M, Antonescu C M, Chang T C, Mendell JT, Salzberg S L.2015.StringTie enables improved recon-struction of a transcriptome from RNA-Seq reads[J].Nature Biotechnology, 33(3):290-295.doi: 10.1038/nbt.3122

Rangan P, Furtado A, Henry R J.2016.New evidence for grain specific C4 photosynthesis in wheat[J].Scientific Reports, 6(1):31721.doi: 10.1038/srep31721.

Salgado J C, Meleiro L P, Carli S, Ward R J.2018.Glucose tolerant and glucose stimulated β-glucosidases-A review[J].Bioresource Technology, 267:704-713.doi: 10.1016/j.bior-tech.2018.07.137.

Simmonds N W.1995.The relation between yield and protein in cereal grain[J].Journal of the Science of Food and Agri-culture, 67(3):309-315.doi: 10.1002/jsfa.2740670306.

Wagner G K, Pesnot T.2010.Glycosyltransferases and their assays[J].ChemBioChem, 11(14):1939-1949.doi: 10.1002/cbic.201000201.

Weegels P L, Hamer R J, Schofield J D.1996.Functional properties of wheat glutenin[J].Journal of Cereal Science, 23(1):1-17.doi: 10.1006/jcrs.1996.0001.

Xiao J, Liu B, Yao Y Y, Guo Z F, Jia H Y, Kong L R, Zhang AM, Ma W J, Ni Z F, Xu S B, Lu F, Jiao Y N, Yang W Y, Lin X L, Sun S L, Lu Z F, Gao L F, Zhao G Y, Cao S H, Chen Q, Zhang K P, Wang M C, Wang M, Hu Z R, Guo WL, Li G Q, Ma X, Li J M, Han F P, Fu X D, Ma Z Q, Wang D W, Zhang X Y, Ling H Q, Xia G M, Tong Y P, Liu Z Y, He Z H, Jia J Z, Chong K.2022.Wheat genomic study for genetic improvement of traits in China[J].Science China Life Sciences, 65(9):1718-1775.doi: 10.1007/s11427-022-2178-7.

Yang M M, Liu Y, Dong J, Zhao W C, Kashyap S, Gao X, Rustgi S, Wen S S.2020.Probing early wheat grain development via transcriptomic and proteomic approaches[J].Functional&Integrative Genomics, 20(1):63-74.doi: 10.1007/s10142-019-00698-9.

Zhang X B, Liu C J.2015.Multifaceted regulations of gateway enzyme phenylalanine ammonia-lyase in the biosynthesis of phenylpropanoids[J].Molecular Plant, 8(1):17-27.doi: 10.1016/j.molp.2014.11.001.

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宁春4号×河东乌麦F_6:7家系籽粒蛋白质性状及候选基因分析

doi: 10.3969/j.issn.2095-1191.2023.12.003

作者简介:
陈佳静(1997-),https://orcid.org/0009-0007-9568-815X,研究方向为作物育种技术,E-mail:chenjiajing1010@163.com

通讯作者:
王掌军(1978-),https://orcid.org/0000-0002-1585-8347,博士,副教授,主要从事小麦遗传育种研究工作,E-mail:wangzj-gs@126.com

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Grain protein traits and candidate genes analysis in F_6:7 pedigrees derived from Ningchun No. 4×Hedong black wheat

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目录

留言板

宁春4号×河东乌麦F6:7家系籽粒蛋白质性状及候选基因分析

doi: 10.3969/j.issn.2095-1191.2023.12.003

作者简介: 陈佳静(1997-),https://orcid.org/0009-0007-9568-815X,研究方向为作物育种技术,E-mail:chenjiajing1010@163.com

通讯作者: 王掌军(1978-),https://orcid.org/0000-0002-1585-8347,博士,副教授,主要从事小麦遗传育种研究工作,E-mail:wangzj-gs@126.com

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出版历程

Grain protein traits and candidate genes analysis in F6:7 pedigrees derived from Ningchun No. 4×Hedong black wheat

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出版历程

目录

宁春4号×河东乌麦F_6:7家系籽粒蛋白质性状及候选基因分析

作者简介:
陈佳静(1997-),https://orcid.org/0009-0007-9568-815X,研究方向为作物育种技术,E-mail:chenjiajing1010@163.com

通讯作者:
王掌军(1978-),https://orcid.org/0000-0002-1585-8347,博士,副教授,主要从事小麦遗传育种研究工作,E-mail:wangzj-gs@126.com

Grain protein traits and candidate genes analysis in F_6:7 pedigrees derived from Ningchun No. 4×Hedong black wheat