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郁金香不同组织内参基因筛选及稳定表达分析

巨秀婷 何金娣 张梦洁 张国莉 史国民 何涛

巨秀婷, 何金娣, 张梦洁, 张国莉, 史国民, 何涛. 郁金香不同组织内参基因筛选及稳定表达分析[J]. 南方农业学报, 2023, 54(11): 3174-3185. doi: 10.3969/j.issn.2095-1191.2023.11.005
引用本文: 巨秀婷, 何金娣, 张梦洁, 张国莉, 史国民, 何涛. 郁金香不同组织内参基因筛选及稳定表达分析[J]. 南方农业学报, 2023, 54(11): 3174-3185. doi: 10.3969/j.issn.2095-1191.2023.11.005
JU Xiu-ting, HE Jin-di, ZHANG Meng-jie, ZHANG Guo-li, SHI Guo-min, HE Tao. Screening and stable expression analysis of internal reference genes in different tissues of Tulipa gesneriana L.[J]. Journal of Southern Agriculture, 2023, 54(11): 3174-3185. doi: 10.3969/j.issn.2095-1191.2023.11.005
Citation: JU Xiu-ting, HE Jin-di, ZHANG Meng-jie, ZHANG Guo-li, SHI Guo-min, HE Tao. Screening and stable expression analysis of internal reference genes in different tissues of Tulipa gesneriana L.[J]. Journal of Southern Agriculture, 2023, 54(11): 3174-3185. doi: 10.3969/j.issn.2095-1191.2023.11.005

郁金香不同组织内参基因筛选及稳定表达分析

doi: 10.3969/j.issn.2095-1191.2023.11.005
基金项目: 

中国科学院“西部之光”人才培养计划项目(中科人字〔2022〕4号);青海省自然科学基金项目(2020-ZJ-937Q)

详细信息
    作者简介:

    巨秀婷(1987-),https://orcid.org/0000-0001-7086-0653,副教授,主要从事观赏植物栽培与育种研究工作,E-mail:juxiuting@163.com

    通讯作者:

    何涛(1972-),https://orcid.org/0000-0002-2154-4029,教授,主要从事植物生物技术研究工作,E-mail:hetaoxn@aliyun.com

  • 中图分类号: S682.263

Screening and stable expression analysis of internal reference genes in different tissues of Tulipa gesneriana L.

Funds: 

Light of the WestTalent Training Project of Chinese Academy of Sciences(Zhongkerenzi[2022]4)

  • 摘要: 【目的】筛选郁金香在盐胁迫条件下稳定表达的内参基因,为开展郁金香耐盐相关基因表达调控相关研究提供理论参考。【方法】以郁金香栽培品种红色印象为材料,于苗期对其进行盐胁迫处理,不同时间(0、2、4、6、8、10、12、24和48 h)采集根、鳞茎和叶,通过实时荧光定量PCR检测候选内参基因在郁金香不同组织中的表达情况,再利用Delta CT计算每个样本的平均Ct值,再结合3个常用内参基因稳定性评估软件(geNorm、NormFinder、BestKeeper)的分析结果,利用RefFinder在线软件综合评价候选内参基因的表达稳定性,并通过目的基因质膜Na+/H+逆向转运蛋白基因(SOS1)和液泡膜Na+/H+逆向转运蛋白基因(NHX1)的表达分析验证内参基因的可靠性。【结果】在15个候选内参基因中筛选到9个可用于郁金香各组织实时荧光定量PCR的内参基因。候选内参基因的Ct值分析结果显示,25S rRNA在不同组织中的平均Ct值最低,表达丰度最高,Ct值分布范围相对集中,是根、鳞茎和叶均能稳定表达的内参基因。geNorm和NormFinder分析结果均显示,18S rRNA均能在根、鳞茎和叶中稳定表达,25S rRNA在根和叶中表现出较好的基因稳定性,NADP基因在鳞茎中基因稳定性较好。BestKeeper分析结果显示,根、鳞茎和叶组织中最佳的内参基因为25S rRNA,同时18S rRNA在根和叶中稳定性较好。结合上述4种分析结果通过RefFinder软件得到各候选基因在郁金香不同组织中的表达稳定性综合排名,其中根组织:25S rRNA>18S rRNA>TUB-6>EF1α>NADP>EF-1α>β-actin;鳞茎组织:NADP>25S rRNA>18S rRNA>EF-1α>EF1α>TUB-6;叶组织:25S rRNA>18S rRNA>EF-1α>CYP>UBC。【结论】9个候选内参基因在郁金香不同组织中的表达情况存在差异,25S rRNA和18S rRNA是在郁金香各组织的不同盐胁迫处理中稳定表达的内参基因,同时使用2个内参基因更有利于得到准确的基因表达定量结果。
  • 产祝龙,向林,王艳平. 2022. 郁金香种质资源、育种进展及种球国产化思考[J]. 华中农业大学学报(自然科学版),41(2):144-150.[Chan Z L,Xiang L,Wang Y P. 2022. Germplasm,breeding and localization of tulip bulbs in China[J]. Journal of Huazhong Agricultural University(Natural Science),41(2):144-150.]doi:10.13300/j. cnki. hnlkxb. 2022.02.017.
    陈首业. 2021. 利用西伯利亚白刺Na+/H+逆向转运蛋白基因提高转基因杨树耐盐性的研究[D]. 呼和浩特:内蒙古大学.[Chen S Y. 2021. Study on salt-tolerance improvement of poplar by transformation using Na+/H+ antiporter genes from Nitraria sibirica Pall[D]. Hohhot:Inner Mongolia University.]
    储文渊,王玉娇,朱东悦,陈竹,严涵薇,项艳. 2017. 盐和干旱胁迫下杨树新内参基因的筛选[J]. 林业科学,53(10):70-79.[Chu W Y,Wang Y J,Zhu D Y,Chen Z,Yan H W, Xiang Y. 2017. Selection of novel reference genes in poplar under salt and drought stresses[J]. Scientia Silvae Sinicae,53(10):70-79.]doi: 10.11707/j.1007-7488.20171008.
    崔玥晗,张艳秋,邢桂梅,吴天宇,张惠华,鲁娇娇,屈连伟. 2020. 郁金香种球繁育与复壮研究进展[J]. 园艺与种苗, 40(9):5-7.[Cui Y H,Zhang Y Q,Xing G M,Wu T Y, Zhang H H,Lu J J,Qu L W. 2020. Research progress on tulip bulb propagation and rejuvenation[J]. Horticulture & Seed,40(9):5-7.]doi:10.16530/j.cnki.cn21-1574/s.2020. 09.003.
    郭 会 敏,顾 春 笋,陈 发 棣,徐 迎 春,刘 兆 磊. 2012. 荷 花NnNHX1 基因耐盐性在转化烟草中的验证[J]. 园艺学报,39(2):323-332.[Guo H M,Gu C S,Chen F D,Xu Y C,Liu Z L. 2012. Salt tolerance verification of lotus NnNHX1 in transformed tobacco[J]. Acta Horticulturae Sinica,39(2):323-332.]doi:10.16420/j.issn.0513-353x. 2012.02.005.
    过雪莹,陈静,张晗,平措央吉,唐东芹. 2022. 腐殖酸及盐胁迫作用下郁金香叶片元素含量的吸收效应[J].安徽农业科 学,50(13):140-143.[Guo X Y,Chen J,Zhang H, Pingcuo Y J,Tang D Q. 2022. Absorption effect of humic acid and salt stress on element content in tulip leaves[J]. Journal of Anhui Agricultural Sciences,50(13):140-143.]doi: 10.3969/j.issn.0517-6611.2022.13.037.
    姜琼,王幼宁,王利祥,孙政玺,李霞. 2015. 盐胁迫下大豆根组织定量PCR分析中内参基因的选择[J]. 植物学报,50(6):754-764.[Jiang Q,Wang Y N,Wang L X,Sun Z X, Li X. 2015. Validation of reference genes for quantitative RT-PCR analysis in soybean root tissue under salt stress[J]. Chinese Bulletin of Botany,50(6):754-764.]doi:10. 11983/CBB14173.
    蒋婷婷,高燕会,童再康. 2015. 石蒜属植物实时荧光定量PCR 内参基因的选择[J]. 园艺学报,42(6):1129-1138.[Jiang T T,Gao Y H,Tong Z K. 2015. Selection of reference genes for quantitative real-time PCR in Lycoris[J]. Acta Horticulturae Sinica,42(6):1129-1138.]doi: 10.16420/j.issn.0513-353x.2014-0999.
    巨秀婷,潘阿青,蒋福娟,唐楠,侯志强. 2019. 郁金香种质资源遗传多样性的ISSR分析[J]. 基因组学与应用生物学, 38(8):3667-3674.[Ju X T,Pan A Q,Jiang F J,Tang N, Hou Z Q. 2019. Genetic diversity of tulip germplasm resource revealed by ISSR markers[J]. Genomics and Applied Biology,38(8):3667-3674.]doi:10.13417/j.gab. 038.003667.
    李荣华,王亚军,方正,赵斌. 2020. 郁金香耐盐性鉴定方法研究[J]. 河南农业科学,49(2):130-135.[Li R H,Wang Y J,Fang Z,Zhao B. 2020. Study on salt tolerance identification method of tulip[J]. Journal of Henan Agricultural Sciences,49(2):130-135.]doi:10.15933/j.cnki.1004-3268. 2020.02.017.
    李铁铮,王金铃,刘晓,王晓晖. 2021. 管花肉苁蓉实时荧光定量 PCR 分析中内参基因的选择和验证[J]. 植物生理学报,57(4):969-981.[Li T Z,Wang J L,Liu X,Wang X H. 2021. Selection and validation of appropriate reference genes for qRT-PCR analysis in Cistanche tubulosa[J]. Plant Physiology Journal,57(4):969-981.]doi: 10.13592/j.cnki.ppj.2019.0440.
    李珠,郭建荣,王宝山. 2018. NaCl处理5个品种郁金香生育期的影响[J]. 山东师范大学学报(自然科学版),33(2):205-208.[Li Z,Guo J R,Wang B S. 2018. Effect of NaCl treatments on the growing period of five varieties of Tulips[J]. Journal of Shandong Normal University(Natural Science),33(2):205-208.]doi:10.3969/j.issn.1001-4748.2018. 02.011.
    梁锦,刘海婷,钟荣,李昊,尹冬梅,刘翔,秦巧平,张志国,段可,倪迪安. 2020. 萱草不同器官实时荧光定量PCR内参基因的筛选[J]. 植物生理学报,56(9):1891-1898.[Liang J,Liu J T,Zhong R,Li H,Yin D M,Liu X,Qin Q P, Zhang Z G,Duan K,Ni D K. 2020. Screening of reference genes for quantitative real-time PCR in different organs of Hemerocallis fulva[J]. Plant Physiology Journal,56(9):1891-1898.]doi: 10.13592/j.cnki.ppj.2020.0089.
    马小英,贾方兴,赵颖岚,赵杰才. 2016. 干旱和盐胁迫中大麦实时定量 PCR 内参基因的筛选[J]. 分子植物育种,14(11):3093-3101.[Ma X Y,Jia F X,Zhao Y L,Zhao J C. 2016. Reference genes screening for quantitative real-time PCR in barley under drought and salt stress[J]. Molecular Plant Breeding,14(11):3093-3101.]doi:10.13271/j.mpb. 014.003093.
    倪萌柯,李笑然,李艺杰,张皓源,许会芬,李明. 2022. 新西兰白兔不同内脏组织中实时荧光定量 PCR 内参基因的筛选[J]. 河南农业大学学报,56(5):807-815.[Ni M K,Li X R,Li Y J,Zhang H Y,Xu H F,Li M. 2022. Screening reference genes by qRT-PCR in different visceral tissues of New Zealand white rabbits[J]. Journal of Henan Agricultural University,56(5):807-815.]doi:10.16445/j.cnki. 1000-2340.20220322.001.
    潘艳艳,张义飞,宿鹏鹍,张大伟,赵曦,潘丽铭. 2021. 郁金香物候期及特性[J]. 江苏农业科学,49(8):137-140.[Pan Y Y,Zhan Y F,Su P K,Zhang D W,Zhao X,Pan L M. 2021. Phenological period and characteristics of tulip[J]. Jiangsu Agricultural Sciences,49(8):137-140.]doi:10. 15889/j.issn.1002-1302.2021.08.024.
    任锐,戴鹏辉,李萌,刘志明,曹福祥. 2016. 珙桐实时定量PCR内参基因的筛选及稳定性评价[J]. 植物生理学报, 52(10):1565-1575.[Ren R,Dai P H,Li M,Liu Z M,Cao F X. 2016. Selection and stability evaluation of reference genes for real-time quantitative PCR in dove tree(Davidia involucrata)[J]. Plant Physiology Journal,52(10):1565-1575.]doi: 10.13592/j.cnki.ppj.2016.0325.
    时丕彪,洪立洲,王军,费月跃,王伟义,吕远大,顾闽峰. 2020. 印度南瓜Na+/H+逆向转运蛋白基因CmaSOS1 的克隆与表达分析[J]. 核农学报,34(12):2638-2646.[Shi P B,Hong L Z,Wang J,Fei Y Y,Wang W Y,Lü Y D,Gu M F. 2020. Cloning and expression analysis of a Na+/H+ antiporter gene CmaSOS1 in Cucurbita maxima[J]. Journal of Nuclear Agricultural Sciences,34(12):2638-2646.]doi: 10.11869/j.issn.100-8551.2020.12.2638.
    宋美玲,黄胜和,陈祖杰,邹嘉轩,刘欢胜,全文军. 2022. 斑地锦RT-qPCR内参基因的筛选[J]. 广西植物,42(2):340-348.[Song M L,Huang S H,Chen Z J,Zou J X,Liu H S, Quan W J. 2022. Screening of reference genes for RTqPCR in Euphorbia maculate[J]. Guihaia,42(2):340-348.]doi: 10.11931/guihaia.gxzw202101081.
    孙燚,张延龙,牛立新. 2008. 低温贮藏期间不同含水量郁金香种球的几种生理生化指标变化[J]. 植物生理学通讯, 44(2):240-242.[Sun Y,Zhang Y L,Niu L X. 2008. Changes in some physiological and biochemical indexes of Tulipa gesneriana L. bulbs with different moisture contents during cold storage period[J]. Plant Physiology Journal,44(2):240-242.]doi: 10.13592/j.cnki.ppj.2008.02.039.
    唐楠. 2014. 郁金香遗传连锁图谱构建及主要真菌病害抗性QTLs定位[D]. 杨凌:西北农林科技大学.[Tang N. 2014.Construction of genetic linkage map for tulip and identification of QTLs for resistance to major fungal diseases[D]. Yangling:Northwest A & F University.]
    王磊,李珠,袁芳,王宝山. 2019. 不同色系郁金香品种的耐盐评价及筛选[J]. 植物生理学报,55(5):657-666.[Wang L,Li Z,Yuan F,Wang B S. 2019. Evaluation and screening of tulips cultivars with different colors[J]Plant Physiology Journal,55(5):657-666.]doi:10.13592/j. cnki. ppj. 2018.0547.
    王迎儿,高旭,王毓洪,宋慧,应泉盛,张香琴,严蕾艳,张宴瑜. 2015. 南瓜耐盐种质的筛选鉴定及耐盐基因的标记[J]. 浙江农业学报,27(3):372-379.[Wang Y E,Gao X, Wang Y H,Song H,Ying Q S,Zhang X Q,Yan L Y, Zhang Y Y. 2015. Screening of salt tolerance germplasms and tagging of the tolerance gene(s)using RAPD and SSR markers in pumpkin[J]Acta Agriculturae Zhejiangensis,27(3):372-379.]doi:10.3969/j.issn.1004-1524.2015. 03.10.
    徐圆圆,赵国春,郝颖颖,翁学煌,陈仲,贾黎明. 2022. 无患子RT-qPCR内参基因的筛选与验证[J]. 生物技术通报,38(10):80-89.[Xu Y Y,Zhao G C,Hao Y Y,Weng X H, Chen Z,Jia L M. 2022. Reference genes selection and validation for RT-qPCR in Sapindus mukorossi[J]. Biotechnology Bulletin,38(10):80-89.]doi: 10.13560/j.cnki.biotech.bull.1985.2021-1616.
    杨延红,宋桂全,李孔栋,姜倩倩,李光耀,孟迎迎. 2022. 郁金香小孢子发育与花粉萌发特性研究[J]. 西北农林科技大学学报(自然科学版),50(6):93-100.[Yang Y H,Song G Q,Li K D,Jiang Q Q,Li G Y,Meng Y Y. 2022. Characteristics of microspore development and pollen germination of Tulipa gesneriana in vitro[J]. Journal of Northwest Agriculture and Forestry University(Natural Science Edition),50(6):93-100.]doi:10.13207/j.cnki.jnwafu.2022. 06.011.
    叶蓉春,唐楠,唐道城,张静. 2019. 不同低温处理对郁金香休眠期鳞茎3种内源激素含量的影响[J]. 植物生理学报, 55(7):1004-1010.[Ye R C,Tang N,Tang D C,Zhang J. 2019. Effect of different temperature treatments on three endogenous hormones in tulip bulbs during dormancy[J]Plant Physiology Journal,55(7):1004-1010.]doi:10.13592/j.cnki.ppj.2018.0584.袁伟,万红建,杨悦俭. 2012. 植物实时荧光定量PCR内参基因的特点及选择[J]. 植物学报,47(4):427-436.[Yuan W,Wan H J,Yang Y J. 2012. Characterization and selection of reference genes for real-time quantitative RT-PCR of plants[J]. Chinese Bulletin of Botany,47(4):427-436.]doi:10.3724/SP.J.1259.2012.00427.
    张佳佳,张力维,李勇鹏,焦晓琳,朱逢玲,杜丽. 2016. 不同非生物胁迫下香樟实时定量 PCR 内参基因的选择[J]. 生物技术通报,32(10):205-211.[Zhang J J,Zhang L W,Li Y P,Jiao X L,Zhu F L,Du L. 2016. Evaluation of candidate reference genes for normalization of quantitative RTPCR in Cinnamomum camphora under various abiotic stresses[J]. Biotechnology Bulletin,32(10):205-211.]doi: 10.13560/j.cnki.biotech.bull.1985.2016.10.025.
    张秋悦,刘昌来,于晓晶,杨甲定,封超年. 2022. 盐胁迫条件下杜梨叶片差异表达基因 qRT-PCR 内参基因筛选[J]. 园艺学报,49(7):1557-1570.[Zhang Q Y,Liu C L,Yu X J,Yang J D,Feng C N. 2022. Screening of reference genes for differentially expressed genes in Pyrus betulaefolia plant under salt stress by qRT-PCR[J]. Acta Horticulturae Sinica,49(7):1557-1570.]doi:10.16420/j.issn.0513-353x. 2021-0376.
    张艳秋,邢桂梅,崔玥晗,代勇,杨迪,屈连伟. 2019. 低温处理时间对郁金香生物学性状及种球繁殖的影响[J]. 北方园艺,(1):103-109.[Zhang Y Q,Xing G M,Cui Y H,Dai Y,Yang D,Qu L W. 2019. Effect of cooling treatment duration on biological traits and propagation of tulip[J]. Northern Horticulture,(1):103-109.]doi:10.11937/bfyy. 20180308.
    张玉芳,赵丽娟,曾幼玲. 2014. 基因表达研究中内参基因的选择与应用[J]. 植物生理学报,50(8):1119-1125.[Zhang Y F,Zhao L J,Zeng Y L. 2014. Selection and application of reference genes for gene expression studies[J]. Plant Physiology Journal,50(8):1119-1125.]doi: 10.13592/j.cnki.ppj.2014.0201.
    章颖佳,程少禹,王卓为,戴梦怡,董彬,张超,张寿洲,王亚玲,申亚梅. 2022. 紫玉兰'红元宝'花芽分化阶段基因定量分析的内参基因筛选[J]. 广西植物,42(1):113-121.[Zhang Y J,Cheng S Y,Wang Z W,Dai M Y,Dong B, Zhang C,Zhang S Z,Wang Y L,Shen Y M. 2022. Selection of refernce genes in Magnolia liliflora'Hongyuanbao'during flower bud differentiation[J]. Guihaia,42(1):113-121.]doi: 10.11931/guihaia.gxzw202012047.
    赵杨静,唐楠,唐道城,张静. 2021. 高效液相色谱法测定郁金香鳞茎中 3 种内源激素[J]. 植物研究,41(1):130-137.[Zhao Y J,Tang N,Tang D C,Zhang J. 2021. An optimized HPLC procedure for analyzing three endogenous hormones in tulip bulbs[J]. Bulletin of Botanical Research, 41(1):130-137.]doi:10.7525/j.issn.1673-5102.2021.01. 016.
    赵艺蕊,黄春颖,王克涛,徐一帆,王建华,邢语琳,陶瞫宸,黄坚钦,李岩. 2022. 山核桃实时荧光定量PCR分析中内参基因的筛选与验证[J]. 果树学报,39(1):10-21.[Zhao Y R,Huang C Y,Wang K T,Xu Y F,Wang J H,Xing Y L, Tao S C,Huang J Q,Li Y. 2022. Screening and verification of internal reference genes by real time quantitative PCR analysis in Carya cathayensis[J]. Journal of Fruit Science, 39(1):10-21.]doi: 10.13925/j.cnki.gsxb.202310289.
    Andersen C L,Jensen J L,Rntoft T F. 2004. Normalization of real-time quantitative reverse transcription-PCR data:A model-based variance estimation approach to identify genes suited for normalization,applied to bladder and colon cancer data sets[J]. American Association for Cancer Research,64(15):5245-5250. doi: 10.1158/0008-5472.CAN-04-0496.
    Cao D,Hou W S,Liu W,Yao W W,Wu C X,Liu X B,Han T F. 2011. Overexpression of TaNHX2 enhances salt tolerance of'composite'and whole transgenic soybean plants[J]. Plant Cell,Tissue & Organ Culture,107(3):541-552.doi: 10.1007/s11240-011-0005-9.
    Dheda K J,Huggett J F,Chang J S,Kim L U,Bustin S A,Johnson M A,Rook G A,Zumla A. 2005. The implications of using an inappropriate reference gene for real-time reverse transcription PCR data normalization[J]. Analytical Biochemistry,344(1):141-143. doi: 10.1016/j.ab.2005.05.022.
    Hou F F,Li S,Wang J Y,Kang X P,Weng Y Q,Xing G M, Huang Y. 2017. Identification and validation of reference genes for quantitative real-time PCR studies in long yellow daylily,Hemerocallis citrina borani[J]. PLoS One,12(3):0174933. doi: 10.1371/journal.pone.0174933.
    Li W,Qian Y Q,Han L,Liu J X,Li Z J,Ju G S,Sun X Y. 2015.Validation of candidate reference genes for gene expression normalization in Buchloe dactyloides using quantitative real-time RT-PCR[J]. Scientia Horticulturae,197:99-106. doi: 10.1016/j.scienta.2015.09.003.
    Liang W J,Cui W N,Ma X L,Wang G,Huang Z J. 2014. Function of wheat Ta-UnP gene in enhancing salt tolerance in transgenic Arabidopsis and rice[J]. Biochemical & Biophysical Research Communications,450(1):794-801. doi: 10.1016/j.bbrc.2014.06.055.
    Livak K J,Schmittgen T. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2-△△Ct method[J]. Methods,25(4):402-408. doi: 10.1006/meth.2001.1262.
    Lou L J,Yu F F,Tian M M,Liu G C,Wu Y R,Wu Y J,Xia R, Pardo J M,Guo Y,Xie Q. 2020. ESCRT-I component VPS23A sustains salt tolerance by strengthening the SOS module in Arabidopsis[J]. Molecular Plant,13(8):1134-1148. doi: 10.1016/j.molp.2020.05.010.
    Pfaffl M W,Tichopad A,Prgomet C,Neuvians T P. 2004.Determination of stable housekeeping genes,differentially regulated target genes and sample integrity:BestkeeperExcel-based tool using pair-wise correlations[J]. Biotechnology Letters,26(6):509-515. doi:10.1023/B:BILE. 0000019559.84305.47.
    Vandesompele J,De Preter K D,Pattyn F,Poppe B,Van Roy N,De Paepe A,Speleman F. 2002. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes[J]. Genome Biology,3(7):research0034.1-0034.11. doi: 10.1186/gb-2002-3-7-resea-rch0034.
    Volkov R A,Panchuk I I,Schöffl F. 2003. Heat-stress dependencyand developmental modulation of gene expression:The potential of house-keeping genes as internal standards in mRNA expression profiling using real-time RT-PCR[J]. Journal of Experimental Botany,54(391):2343-2349. doi: 10.1093/jxb/erg244.
    Yeap W C,Loo J M,Wong Y C,Kulaveerasingam H. 2014.Evaluation of suitable reference genes for qRT-PCR gene expression normalization in reproductive,vegetative tissues and during fruit development in oil palm[J]. Plant Cell Tissue and Organ Culture,116(1):55-66. doi: 10.1007/s11240-013-0382-3
    Zhang W D,Wang P,Bao Z,Ma Q,Duan L J,Bao A K,Zhang J L,Wang S M. 2017. SOS1,HKT1;5,and NHX1 synergistically modulate Na+ homeostasis in the halophytic grass Puccinellia tenuiflora[J]. Frontiers in Plant Science, 8:576. doi: 10.3389/fpls.2017.00576.
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出版历程
  • 收稿日期:  2023-01-12
  • 网络出版日期:  2024-03-28

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