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妮娜皇后葡萄需冷量及休眠解除过程中相关基因表达分析

黄泳碧 覃园媛 陆秀边 白扬 韩佳宇 曹雄军 白先进 廖原 张延晖 王博

黄泳碧, 覃园媛, 陆秀边, 白扬, 韩佳宇, 曹雄军, 白先进, 廖原, 张延晖, 王博. 妮娜皇后葡萄需冷量及休眠解除过程中相关基因表达分析[J]. 南方农业学报, 2023, 54(11): 3165-3173. doi: 10.3969/j.issn.2095-1191.2023.11.004
引用本文: 黄泳碧, 覃园媛, 陆秀边, 白扬, 韩佳宇, 曹雄军, 白先进, 廖原, 张延晖, 王博. 妮娜皇后葡萄需冷量及休眠解除过程中相关基因表达分析[J]. 南方农业学报, 2023, 54(11): 3165-3173. doi: 10.3969/j.issn.2095-1191.2023.11.004
HUANG Yong-bi, QIN Yuan-yuan, LU Xiu-bian, BAI Yang, HAN Jia-yu, CAO Xiong-jun, BAI Xian-jin, LIAO Yuan, ZHANG Yan-hui, WANG Bo. Chilling requirement and related gene expression analysis during dormancy release of Queen Nina grape[J]. Journal of Southern Agriculture, 2023, 54(11): 3165-3173. doi: 10.3969/j.issn.2095-1191.2023.11.004
Citation: HUANG Yong-bi, QIN Yuan-yuan, LU Xiu-bian, BAI Yang, HAN Jia-yu, CAO Xiong-jun, BAI Xian-jin, LIAO Yuan, ZHANG Yan-hui, WANG Bo. Chilling requirement and related gene expression analysis during dormancy release of Queen Nina grape[J]. Journal of Southern Agriculture, 2023, 54(11): 3165-3173. doi: 10.3969/j.issn.2095-1191.2023.11.004

妮娜皇后葡萄需冷量及休眠解除过程中相关基因表达分析

doi: 10.3969/j.issn.2095-1191.2023.11.004
基金项目: 

国家自然科学基金项目(31960572);广西重点研发计划项目(桂科 AB21196042);广西自然科学基金项目(2023GXNSFBA026047)

详细信息
    作者简介:

    黄泳碧(1998-),https://orcid.org/0009-0001-6634-9323,研究方向为果树栽培生理及分子调控,E-mail:huangyb7683@163.com

    通讯作者:

    王博(1985-),https://orcid.org/0000-0002-0666-8509,博士,主要从事葡萄栽培生理及分子研究工作,E-mail:wangbo0127@163.com

  • 中图分类号: S663.101

Chilling requirement and related gene expression analysis during dormancy release of Queen Nina grape

Funds: 

National Natural Science Foundation of China(31960572)

  • 摘要: 【目的】分析妮娜皇后葡萄需冷量及休眠解除过程中相关基因表达,为该品种在广西葡萄产区引种推广及其休眠解除机制的研究提供理论参考。【方法】以3年生妮娜皇后葡萄为试验材料,采用0~7.2 ℃估算模型,对采后修剪枝段进行(4±1)℃下0、312、408和504 h共4个不同时长的低温处理,通过水培调查计算各低温时长处理枝段萌芽率。并对比3%浓度单氰胺破眠剂处理下的枝段萌芽率确定其需冷量范围;测定各处理枝段中可溶性总糖与淀粉含量;通过实时荧光定量PCR检测芽内5个芽休眠解除关键基因的相对表达量。【结果】妮娜皇后葡萄低温处理312 h后,水培4周萌芽率均高于50%,且均与相同低温处理取样后进行单氰胺处理的萌芽率无显著差异(P>0.05),结合采样前自然条件下0~7.2 ℃的低温积累时数37 h,将低温处理时间与自然积累的低温时数相加估算其需冷量<349 h;妮娜皇后葡萄芽休眠解除过程中,枝条中的淀粉含量显著降低(P<0.05,下同),淀粉转化为可溶性糖,可溶性糖含量显著增加。与未经低温处理(0 h)相比,低温处理后妮娜皇后芽中脱落酸(ABA)合成酶基因VvNCED1、ABA降解酶基因VvA8H4、ABA调节的胼胝质合成酶基因VvCALS1和胼胝质水解酶基因VvGLU1等4个关键休眠基因的相对表达量均显著增加,ABA合成酶基因VvXERICO相对表达量显著降低。【结论】低温处理能通过降低妮娜皇后葡萄枝条中淀粉含量,增加可溶性总糖含量,并进一步上调葡萄芽内ABA降解酶基因和胼胝质水解酶基因的表达解除芽休眠。妮娜皇后葡萄为低需冷量品种,适宜在广西葡萄产区种植。
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  • 收稿日期:  2023-06-15
  • 网络出版日期:  2024-03-28

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