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外源生长素影响百合地下茎生鳞茎发生的转录组分析

杨成龙 方少忠 黄永旺 陈佩振 林智敏

杨成龙, 方少忠, 黄永旺, 陈佩振, 林智敏. 外源生长素影响百合地下茎生鳞茎发生的转录组分析[J]. 南方农业学报, 2023, 54(11): 3156-3164. doi: 10.3969/j.issn.2095-1191.2023.11.003
引用本文: 杨成龙, 方少忠, 黄永旺, 陈佩振, 林智敏. 外源生长素影响百合地下茎生鳞茎发生的转录组分析[J]. 南方农业学报, 2023, 54(11): 3156-3164. doi: 10.3969/j.issn.2095-1191.2023.11.003
YANG Cheng-long, FANG Shao-zhong, HUANG Yong-wang, CHEN Pei-zhen, LIN Zhi-min. Transcriptome analysis of exogenous auxin effect on lily underground stem bulbs formation[J]. Journal of Southern Agriculture, 2023, 54(11): 3156-3164. doi: 10.3969/j.issn.2095-1191.2023.11.003
Citation: YANG Cheng-long, FANG Shao-zhong, HUANG Yong-wang, CHEN Pei-zhen, LIN Zhi-min. Transcriptome analysis of exogenous auxin effect on lily underground stem bulbs formation[J]. Journal of Southern Agriculture, 2023, 54(11): 3156-3164. doi: 10.3969/j.issn.2095-1191.2023.11.003

外源生长素影响百合地下茎生鳞茎发生的转录组分析

doi: 10.3969/j.issn.2095-1191.2023.11.003
基金项目: 

福建省自然科学基金项目(2023J01366)

详细信息
    作者简介:

    杨成龙(1990-),https://orcid. org/0009-0008-1075-0119,主要从事百合生长发育与花期调控研究工作,E-mail:yangchenglong-sws@faas.cn

    通讯作者:

    林智敏(1976-),https://orcid.org/0000-0002-4830-253X,博士,副研究员,主要从事百合分子育种研究工作,E-mail:lzmfaas@sina.com

  • 中图分类号: S682.265;Q943.2

Transcriptome analysis of exogenous auxin effect on lily underground stem bulbs formation

Funds: 

Fujian Natural Science Foundation(2023J01366)

  • 摘要: 【目的】揭示激素调控百合地下茎生鳞茎发生发育的分子机理,为深入开展生长素相关基因调节百合地下茎生鳞茎发生分子机制研究提供参考依据。【方法】通过0.2和2.0 mmol/L浓度的外源生长素吲哚乙酸(IAA)对百合地下茎部进行处理,以清水处理作为对照(CK),对收获的地下茎生鳞茎的数量和重量进行测定,结合转录组测序分析,揭示不同浓度生长素处理下各激素相关基因的时空表达模式,并利用实时荧光定量PCR对百合侧生器官发生关键基因进行表达水平检测。【结果】2.0 mmol/L浓度IAA处理后的百合植株,在茎生鳞茎总数量、单株茎生鳞茎数量和茎生鳞茎平均克重均低于0.2 mmol/L处理和CK。KEEG富集结果指向植物激素信号转导通路,差异表达基因(DEGs)数字表达谱显示,几乎所有与植物激素相关的基因均受影响,包括生长素、乙烯、脱落酸、油菜素内酯、茉莉酸甲酯、细胞分裂素和赤霉素,其中茉莉酸甲酯相关的DEGs最多,其次为生长素。实时荧光定量PCR检测表明,2.0 mmol/L处理中WOX9WOX11、KNOX2CYCD3CYCD5基因表达水平均显著低于CK(P<0.05)。【结论】高浓度生长素IAA可有效迟滞百合地下茎生鳞茎的形成和发育。外源生长素处理不仅影响多种植物激素的合成代谢、运输和信号转导,也抑制百合侧生器官发生关键基因的表达,从而影响地下茎生鳞茎的形成和发育。
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出版历程
  • 收稿日期:  2023-08-28
  • 网络出版日期:  2024-03-28

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