杨成龙, 方少忠, 黄永旺, 陈佩振, 林智敏. 2023: 外源生长素影响百合地下茎生鳞茎发生的转录组分析. 南方农业学报, 54(11): 3156-3164. DOI: 10.3969/j.issn.2095-1191.2023.11.003
引用本文: 杨成龙, 方少忠, 黄永旺, 陈佩振, 林智敏. 2023: 外源生长素影响百合地下茎生鳞茎发生的转录组分析. 南方农业学报, 54(11): 3156-3164. DOI: 10.3969/j.issn.2095-1191.2023.11.003
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YANG Cheng-long, FANG Shao-zhong, HUANG Yong-wang, CHEN Pei-zhen, LIN Zhi-min. 2023: Transcriptome analysis of exogenous auxin effect on lily underground stem bulbs formation. Journal of Southern Agriculture, 54(11): 3156-3164. DOI: 10.3969/j.issn.2095-1191.2023.11.003
Citation: YANG Cheng-long, FANG Shao-zhong, HUANG Yong-wang, CHEN Pei-zhen, LIN Zhi-min. 2023: Transcriptome analysis of exogenous auxin effect on lily underground stem bulbs formation. Journal of Southern Agriculture, 54(11): 3156-3164. DOI: 10.3969/j.issn.2095-1191.2023.11.003
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外源生长素影响百合地下茎生鳞茎发生的转录组分析

Transcriptome analysis of exogenous auxin effect on lily underground stem bulbs formation

    摘要
  • 摘要: 【目的】揭示激素调控百合地下茎生鳞茎发生发育的分子机理，为深入开展生长素相关基因调节百合地下茎生鳞茎发生分子机制研究提供参考依据。【方法】通过0.2和2.0 mmol/L浓度的外源生长素吲哚乙酸（IAA）对百合地下茎部进行处理，以清水处理作为对照（CK），对收获的地下茎生鳞茎的数量和重量进行测定，结合转录组测序分析，揭示不同浓度生长素处理下各激素相关基因的时空表达模式，并利用实时荧光定量PCR对百合侧生器官发生关键基因进行表达水平检测。【结果】2.0 mmol/L浓度IAA处理后的百合植株，在茎生鳞茎总数量、单株茎生鳞茎数量和茎生鳞茎平均克重均低于0.2 mmol/L处理和CK。KEEG富集结果指向植物激素信号转导通路，差异表达基因（DEGs）数字表达谱显示，几乎所有与植物激素相关的基因均受影响，包括生长素、乙烯、脱落酸、油菜素内酯、茉莉酸甲酯、细胞分裂素和赤霉素，其中茉莉酸甲酯相关的DEGs最多，其次为生长素。实时荧光定量PCR检测表明，2.0 mmol/L处理中WOX9、WOX11、KNOX2、CYCD3和CYCD5基因表达水平均显著低于CK（P<0.05）。【结论】高浓度生长素IAA可有效迟滞百合地下茎生鳞茎的形成和发育。外源生长素处理不仅影响多种植物激素的合成代谢、运输和信号转导，也抑制百合侧生器官发生关键基因的表达，从而影响地下茎生鳞茎的形成和发育。

     

    Abstract: 【Objective】To reveal the molecular mechanism of hormone regulation on the occurrence and development of lily underground stem bulbs,and to provide reference for further development of the molecular mechanism of auxin related genes regulation of lily underground stem bulbs.【Method】The underground stems of lily were treated with exogenous auxin indoleacetic acid(IAA)at concentrations of 0.2 and 2.0 mmol/L,and water treatment was used as the control(CK). The quantity and weight of harvested underground stem bulbs were measured. Combined with transcriptome sequencing analysis,the temporal and spatial expression patterns of various hormone-related genes under different auxin concentrations were revealed. Real-time fluorescence quantitative PCR was used to detect the expression level of key genes in lily lateral organogenesis.【Result】The total number of stem bulbs,number of stem bulbs per plant and average gram weight of stem bulbs of lily plants treated with 2.0 mmol/L concentration IAA were lower than those treated with 0.2 mmol/L concentration auxin and control group. The results of KEEG enrichment pointed to the plant hormone signal transduction pathway. The digital expression profiles of differentially expressed genes(DEGs)showed that almost all genes related to plant hormones were affected,including auxin,ethylene,ababolic acid,brassinolide,methyl jasmonate,cytokinin and gibberellin,among which methyl jasmonate had the most DEGs,followed by auxin. Real-time fluorescence quantitative PCR detection showed that the expression levels of WOX9,WOX11,KNOX2,CYCD3 and CYCD5 genes in 2.0 mmol/L treatment were significantly lower than those in CK(P<0.05).【Conclusion】High concentration of auxin IAA can effectively delay the formation and development of underground stem bulbs of lily. Exogenous auxin treatment not only affects the anabolism,transport and signal transduction of many plant hormones,but also inhibits the expression of key genes of lily lateral organogenesis,thus affecting the formation and development of underground stem bulbs.

     

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