郜秋艳, 李美凤, 尹杰, 陈猛, 张金玉, 邓燕莉, 张静, 刘建军. 2021: 不同贮藏年限武夷岩茶化学成分差异及其抗氧化活性比较. 南方农业学报, 52(7): 1952-1959. DOI: 10.3969/j.issn.2095-1191.2021.07.025
引用本文: 郜秋艳, 李美凤, 尹杰, 陈猛, 张金玉, 邓燕莉, 张静, 刘建军. 2021: 不同贮藏年限武夷岩茶化学成分差异及其抗氧化活性比较. 南方农业学报, 52(7): 1952-1959. DOI: 10.3969/j.issn.2095-1191.2021.07.025
GAO Qiu-yan, LI Mei-feng, YIN Jie, CHEN Meng, ZHANG Jin-yu, DENG Yan-li, ZHANG Jing, LIU Jian-jun. 2021: Comparative analysis on chemical components and antioxidant activity for aged Wuyi rock teas in different years. Journal of Southern Agriculture, 52(7): 1952-1959. DOI: 10.3969/j.issn.2095-1191.2021.07.025
Citation: GAO Qiu-yan, LI Mei-feng, YIN Jie, CHEN Meng, ZHANG Jin-yu, DENG Yan-li, ZHANG Jing, LIU Jian-jun. 2021: Comparative analysis on chemical components and antioxidant activity for aged Wuyi rock teas in different years. Journal of Southern Agriculture, 52(7): 1952-1959. DOI: 10.3969/j.issn.2095-1191.2021.07.025

不同贮藏年限武夷岩茶化学成分差异及其抗氧化活性比较

Comparative analysis on chemical components and antioxidant activity for aged Wuyi rock teas in different years

  • 摘要: 【目的】对不同贮藏年限武夷岩茶的丙烯酰胺和品质成分含量及抗氧化能力进行差异分析,为武夷岩茶的食品安全、加工和储藏提供理论依据。【方法】以矮脚乌龙武夷岩茶为研究对象,采用超高效液相色谱法和分光光度法检测不同贮藏年限0(新茶)、2、4和6年武夷岩茶的丙烯酰胺含量及游离氨基酸等主要品质化学成分含量,测定其1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除能力,并对不同贮藏年限武夷岩茶丙烯酰胺、主要品质化学成分和抗氧化能力进行相关分析。【结果】贮藏0(新茶)、2、4和6年的武夷岩茶丙烯酰胺含量总体较低,分别为304.67、255.09、188.19和172.45 ng/g,均在欧盟法规安全限定范围内(≤850 ng/g)。与新茶相比,贮藏2、4和6年武夷岩茶茶样中丙烯酰胺含量均极显著降低(P<0.01,下同);贮藏2年茶样的游离氨基酸含量为2.85%,较新茶显著降低44.5%(P<0.05,下同);随着贮藏时间的延长,武夷岩茶中茶多酚、儿茶素、咖啡碱和水浸出物含量及抗氧化活性均呈下降趋势,而可溶性总糖含量呈上升趋势。Pearson相关分析结果表明,武夷岩茶中丙烯酰胺、咖啡碱和儿茶素含量与贮藏时间呈极显著负相关,游离氨基酸含量与贮藏时间呈显著负相关,儿茶素含量与丙烯酰胺含量呈极显著正相关;游离氨基酸含量与抗氧化活性呈极显著负相关。【结论】随着贮藏年限的增加,武夷岩茶主要化学成分大多呈下降趋势;丙烯酰胺含量较低,不会对人体构成风险,消费者可放心饮用。

     

    Abstract: 【Objective】 In order to provide a theoretical basis for the food safety, processing, storage of Wuyi rock tea, the content of acrylamide, quality component content and antioxidant capacity of Wuyi rock tea in different storage years were analyzed.【Method】 In this study, main chemical compositions contents such as acrylamide content and free amino acid and the 1, 1-diphenyl-2-trinitrophenylhydrazine(DPPH) radical scavenging abilityof short leg oolong Wuyi rock tea with different storage years0(new tea), 2, 4 and 6 yearswere determined by ultra high performance liquid chromatography(UHPLC) and spectrophotometry. The correlation between acrylamide, main quality chemical compositions and antioxidant capacity of Wuyi rock tea in different storage years was analyzed.【Result】 The results showed that the contents of acrylamide of Wuyi rock tea with 0(new tea), 2, 4 and 6 years were low, which were 304.67, 255.09, 188.19, 172.45 ng/g, respectively, and they were all within the safety limit of EU regulations(≤850 ng/g). The contents of acrylamidein storage period of 2, 4 and 6 years were declined extremely compared with the fresh tea(P<0.01, the same below). The content of free amino acids was 2.85% after storage for 2 years, which was significantly lower than new tea by 44.5%(P<0.05, the same below). With the increase of storage years, the contents of tea polyphenols, catechins, caffeine, moisture extracts and antioxidant activity showed a downward trend at each storage year, while the opposite trend was observed for total sugar contents. Pearson correlation analysis showed that there was extremely significant positive correlation between acrylamide, caffeine, catechins and storage time, free amino acids content was significantly negatively correlated with storage time, catechins content was extremely positively correlated with acrylamide content, and free amino acids content was extremely negatively correlated with antioxidant activity.【Conclusion】 With the increase of storage years, most of the main chemical components of Wuyi rock tea show a downward trend. The content of acrylamide in Wuyi rock tea is low and will not pose risk to human body, so consumers can drink it safely.

     

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