Abstract: 【Objective】 To provide a theoretical basis for the use of glume-opening accelerator to regulate flowering in seed production, the effect of glume-opening accelerator RHS₁ on flowering habits of two Indica male sterile lines(CMS) lines with Japonica blood was researched.【Method】 Using the Indica CMS lines Shennong 4 A and 18 A with Japonica blood as the materials, using the single-factor completely randomized block design method, and treating with 0.1 ml/L of glume-opening accelerator RHS₁ and clear water control(CK), the dynamics of single-spike flowering time, single-spike flowering duration, single-spike daily flowering dynamics, glume opening angle, glume spacing, necking rate, stigma exposure rate, and closure situation of different tested materials were investigated and compared.【Result】 The results showed that the flower opening accelerator RHS₁ could not only advance flower time of text materials and increase flower time concentration, but also increase flower period concentration. Compared with clearwater control treatment, the flowering time treated by RHS₁ was advanced 0.5 h, the flowering time concentration(measured by flowering rate before noon) treated by RHS₁ was increased by 3.72% to 13.55%, and the flowering time concentration treated by RHS₁(measured by the flowering period) was advanced 0.10 to 4.64 d;but there were differences in the sensitivity of the response to flowering time and flowering period among different materials. Compared with water clear control treatment, there were no significant differences in the glume opening angle, glume spacing, stigma exposure rate, necking rate and closure situation of the agent RHS₁(P>0.05). 【Conclusion】 The flowering opening accelerator RHS₁ can be used as one of the commonly used agents for adjusting flowering time and be promoted in production under the premise of mature supporting technology.