Abstract: 【Objective】 By identifying the members of the mango vascular plant one-zinc finger protein(VOZ) gene family members and conducting bioinformatics analysis on them, it laid a foundation for exploring the biological functions of mango VOZ transcription factors in immune response.【Method】 Based on mango genome-wide data, bioinformatics methods were used to identify members of the mango VOZ transcription factor gene family members, and to analyze their physical and chemical properties, conservative motifs, and phylogenetic evolution. Real-time fluorescent quantitative PCR was used to detect the relative expression levels of Colletotrichum gloeosporioides and Xanthomonas citri pv. mangiferaeindicae infection.【Result】 Four VOZ transcription factor family members were identified from the whole mango genome, namely MiVOZ1, MiVOZ2, MiVOZ3 and MiVOZ4 genes. The open reading frame(ORF) length was 1290-1482 bp, the number of encoded amino acids was 429-493, and the protein molecular weight was 47.26-54.96 kD, isoelectric point(pI) was 5.47-6.00, affinity/hydrophobicity index was -0.663 to -0.552, instability index was 37.66-50.86, secondary structure was mainly random coil and α-helix element. Mango and 49 VOZ proteins of 9 other species were clustered into ten major groups. In Class Ⅰ, three MiVOZs proteins(MiVOZ2, MiVOZ3, MiVOZ4) were clustered with apple, cassava and Populus trichocarpa. In Class Ⅴ, MiVOZ1 was clustered with Arabidopsis thaliana, cassava and P.trichocarpa. Under the infection of C. gloeosporioides, only the relative expression of MiVOZ1 and MiVOZ2 genes were significantly higher than the control(P<0.05, the same below); under X. citri pv. mangiferaeindicae infection, only the relative expression of MiVOZ2 gene was significantly higher than that of the control.【Conclusion】 Mango VOZ transcription factors have different biological functions in resisting different pathogens. Among them, the MiVOZ2 gene has similar biological functions in the immune response to resist the infection of C. gloeosporioides and X. citri pv. mangiferaeindicae, and is a positive regulator.