Abstract: ObjetiveWhether cattle in Guangxi have been infected by bovine papillomavirus(BPV)was investigated to provide reference for formulating prevention and control measures of BPV in the future.MethodTumor-shaped sam-ples were collected from the skin of young cattle in a cattle farm in Longan,Guangxi.Based on clinical observation and histopathological observation,BPV were identified and their genotypes were classified by PCR.Then five pairs of speci-fic primers were designed to amplify and sequence BPV genome according to the previous results.ResultHistopathologi-cal slices of the tumor-shaped tissues revealed that,it had epidermis and dermis hyperplasia,and epidermal cells keratini-zation and cavitation occurred.The cells were fusiform and stellate.One strain was isolated from the tumor-shaped sam-ple. Its homology with BPV-1 was 99.0%,and named BPV-GX-LA150909.The full-length genome of strain BPV-GX-LA150909 was 7946 bp,and its GenBank accession number was MF435918.It included three regions:early gene region (E),late gene region(L)and non-coding region(NCR).The homology between strain BPV-GX-LA150909 and BPV-1 type in both genome and L1 gene was the highest,especially with Guizhou strain BPV-1 GZLZ(genome homology 99.9%,L1 gene homology 99.7%). In addition,phylogenetic tree showed that genetic distance of this strain was also close to BPV-1 GZLZ.L1 protein of BPV-GX-LA150909 presented potential B cell epitope,and contained seventeen O-glycosylation sites and one N-glycosylation site.ConclusionBPV-1 infection has been found in Guangxi cattle,and the homology with BPV-1 type at home and abroad is high,which indicates that cross-regional transmission of BPV is a prob-lem that cannot be ignored.